breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,910 T→G W30G (TGG→GGG)  folA → type 3 dihydrofolate reductase
RA 1,613,393 A→T D6V (GAT→GTT)  marR → multiple antibiotic resistance transcriptional regulator MarR
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
RA 3,511,988 C→A E146* (GAG→TAG)  aroK ← shikimate kinase AroK

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356981 357576 596 2 [0] [1] 2 [lacA] [lacA]
* * ÷ NZ_CP009273 380412 380420 9 6 [0] [0] 2 BW25113_RS01880/tauA PIG‑L family deacetylase/taurine ABC transporter substrate‑binding protein
* * ÷ NZ_CP009273 950209 950250 42 2 [1] [1] 2 focA/ycaO formate transporter FocA/30S ribosomal protein S12 methylthiotransferase accessory factor YcaO
* * ÷ NZ_CP009273 1032537 1032598 62 3 [1] [1] 2 hyaF hydrogenase expression/formation protein
* * ÷ NZ_CP009273 1417992 1418045 54 3 [1] [1] 3 [trkG] [trkG]
* * ÷ NZ_CP009273 1459418 1459529 112 2 [1] [1] 3 BW25113_RS25580 BW25113_RS25580
* * ÷ NZ_CP009273 1580836 1580887 52 2 [0] [1] 2 ydeP/ydeQ acid resistance putative oxidoreductase YdeP/fimbrial protein
* * ÷ NZ_CP009273 1633185 1633206 22 2 [1] [1] 2 cspI/rzpQ cold shock protein CspI/DUF2514 domain‑containing protein
* * ÷ NZ_CP009273 2027304 2027387 84 3 [1] [1] 2 drpB/ompC cell division protein DrpB/porin OmpC
* * ÷ NZ_CP009273 2101613 2101651 39 2 [1] [1] 3 glf UDP‑galactopyranose mutase
* * ÷ NZ_CP009273 2144481 2144513 33 2 [1] [1] 2 yegI/yegJ protein kinase YegI/DUF2314 domain‑containing protein
* * ÷ NZ_CP009273 2300296 2300341 46 3 [1] [1] 3 mqo/yojI malate dehydrogenase (quinone)/microcin J25 efflux ABC transporter YojI
* * ÷ NZ_CP009273 2336913 2336918 6 4 [0] [0] 2 yfaL AIDA‑I family autotransporter adhesin YfaL/EhaC
* * ÷ NZ_CP009273 2778510 2778538 29 2 [1] [1] 2 BW25113_RS25705/BW25113_RS13825 DUF5507 domain‑containing protein/tRNA‑Ile
* * ÷ NZ_CP009273 2980529 2980544 16 5 [1] [1] 3 yqeG/yqeH amino acid permease/helix‑turn‑helix transcriptional regulator
* * ÷ NZ_CP009273 3129918 3129955 38 2 [0] [1] 2 pitB/gss inorganic phosphate transporter PitB/bifunctional glutathionylspermidine amidase/synthase
* * ÷ NZ_CP009273 3262680 3262707 28 2 [1] [0] 2 yhaC YhaC family protein
* * ÷ NZ_CP009273 3448796 3448828 33 2 [0] [1] 3 gspA/gspC type II secretion system protein GspA/type II secretion system protein GspC

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 15391 =NA (NA)31 (1.570) 25/158 0.0 84.5% intergenic (+93/‑54) dnaJ/BW25113_RS00080 molecular chaperone DnaJ/IS4‑like element IS421 family transposase
?NZ_CP009273 = 454255 6 (0.290)intergenic (+99/‑89) clpX/lon ATP‑dependent protease ATP‑binding subunit ClpX/endopeptidase La
* ? NZ_CP009273 = 287112NA (NA)14 (0.670) 11/166 0.6 87.5% intergenic (‑55/‑99) BW25113_RS01405/BW25113_RS01410 IS1‑like element IS1B family transposase/hypothetical protein
?NZ_CP009273 1903038 = 2 (0.100)intergenic (‑15/‑144) mgrB/yobH PhoP/PhoQ regulator MgrB/YobH family protein
* ? NZ_CP009273 360104 =NA (NA)22 (1.060) 18/166 0.2 40.0% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 33 (1.590)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 454244 =7 (0.340)29 (1.470) 16/158 0.2 81.3% intergenic (+88/‑100) clpX/lon ATP‑dependent protease ATP‑binding subunit ClpX/endopeptidase La
?NZ_CP009273 = 604803 NA (NA)intergenic (+170/+112) BW25113_RS03025/entD IS4‑like element IS421 family transposase/enterobactin synthase subunit EntD
* ? NZ_CP009273 = 1046001NA (NA)14 (0.670) 11/166 0.6 82.3% intergenic (+15/+418) BW25113_RS05135/cspH IS1‑like element IS1D family transposase/cold shock‑like protein CspH
?NZ_CP009273 = 1903046 3 (0.140)intergenic (‑23/‑136) mgrB/yobH PhoP/PhoQ regulator MgrB/YobH family protein
* ? NZ_CP009273 1203246 =14 (0.670)8 (0.480) 7/134 0.8 52.9% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 3 (0.180)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 1391494NA (NA)5 (0.240) 3/164 1.9 83.5% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 1 (0.050)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727510 (0.000)29 (1.400) 19/166 0.1 100% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 = 45325076 (0.290)6 (0.320) 5/148 1.2 39.6% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 13 (0.700)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA