breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 59 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273822,9550TG68.8% 0.3 / 11.5 16T495P (ACC→CCC) ybhFATP‑binding cassette domain‑containing protein
*NZ_CP0092734,594,1610CT67.7% ‑1.6 / 27.6 31S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092734,594,1670CT64.5% ‑3.4 / 29.3 31S64F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092731,782,8010AC57.9% 20.2 / 23.4 19D37A (GAC→GCC) aroH3‑deoxy‑7‑phosphoheptulonate synthase AroH
*NZ_CP0092731,906,0070TG57.1% 19.2 / 24.5 21H276P (CAC→CCC) htpXprotease HtpX
*NZ_CP0092731,125,8250TC55.6% 13.7 / 20.2 18G165G (GGA→GGGflgAflagellar basal body P‑ring formation chaperone FlgA
*NZ_CP0092731,782,7970AC55.0% 21.6 / 27.2 20T36P (ACC→CCC) aroH3‑deoxy‑7‑phosphoheptulonate synthase AroH
*NZ_CP009273700,0260TG54.5% 16.1 / 24.3 22G209G (GGT→GGGnagEPTS N‑acetyl glucosamine transporter subunit IIABC
*NZ_CP009273892,8050TG54.5% 11.1 / 12.9 11V89G (GTG→GGG) potIputrescine ABC transporter permease PotI
*NZ_CP0092731,571,3800AC54.5% 35.6 / 59.9 44V166G (GTA→GGA) yddBTonB‑dependent receptor plug domain‑containing protein
*NZ_CP009273497,0200AC53.8% 8.2 / 16.3 13*559E (TAA→GAA) ybaLKef family K(+) transporter
*NZ_CP009273997,4470TG53.8% 23.6 / 35.0 26V62G (GTA→GGA) elfGfimbrial protein
*NZ_CP0092732,738,4480AC53.3% 23.0 / 41.2 30V84G (GTG→GGG) trmDtRNA (guanosine(37)‑N1)‑methyltransferase TrmD
*NZ_CP0092732,840,7990AC53.3% 15.5 / 18.4 15V601G (GTG→GGG) hycCformate hydrogenlyase subunit 3
*NZ_CP009273700,0310TG52.9% 11.1 / 14.6 17V211G (GTT→GGT) nagEPTS N‑acetyl glucosamine transporter subunit IIABC
*NZ_CP0092731,329,9480TG52.2% 19.9 / 25.4 23intergenic (+24/‑140)BW25113_RS25560/acnAhypothetical protein/aconitate hydratase AcnA
*NZ_CP00927371,5610TG52.0% 31.6 / 39.2 25N136T (AAC→ACC) thiBthiamine ABC transporter substrate binding subunit
*NZ_CP00927371,5660CT52.0% 6.7 / 34.0 25L134L (CTG→CTAthiBthiamine ABC transporter substrate binding subunit
*NZ_CP0092733,659,0620CA50.0% 26.7 / 22.1 30A37S (GCG→TCG) gadXacid resistance transcriptional activator GadX
*NZ_CP009273687,3030AG47.1% 30.2 / 10.7 34intergenic (‑63/+27)corC/ybeYCNNM family magnesium/cobalt transport protein CorC/rRNA maturation RNase YbeY

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 = 73442823 (0.710)3 (0.100) 3/158 2.5 12.7% intergenic (+122/‑29) ybfD/ybgA ISAs1 family transposase/YbgA family protein
?NZ_CP009273 734433 = 19 (0.610)intergenic (+127/‑24) ybfD/ybgA ISAs1 family transposase/YbgA family protein