breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 141 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,141,2140TG73.1% 4.6 / 17.1 26G20G (GGT→GGGyegDmolecular chaperone
*NZ_CP0092731,152,7270TG66.7% 9.8 / 24.6 27G165G (GGT→GGGycfHmetal‑dependent hydrolase
*NZ_CP009273997,4470TG61.5% 11.3 / 24.9 26V62G (GTA→GGA) elfGfimbrial protein
*NZ_CP0092733,126,6610TG60.0% 13.7 / 31.0 25H219P (CAC→CCC) yghSdTMP kinase
*NZ_CP009273353,6010CA59.7% 0.2 / 60.7 57L267F (TTG→TTTcynRtranscriptional regulator CynR
*NZ_CP009273760,1870GC59.3% 18.4 / 38.8 27P184P (CCG→CCCsucDsuccinate‑‑CoA ligase subunit alpha
*NZ_CP0092734,594,1610CT57.8% 11.9 / 77.2 66S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP009273760,1810GC55.6% 22.0 / 39.2 27P182P (CCG→CCCsucDsuccinate‑‑CoA ligase subunit alpha
*NZ_CP0092734,594,1670CT53.8% 22.9 / 84.3 66S64F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092731,129,3050GA47.1% 37.9 / 10.5 34L16L (CTG→CTAflgFflagellar basal‑body rod protein FlgF
*NZ_CP0092731,129,3130AG46.9% 29.8 / 11.0 32Q19R (CAG→CGG) flgFflagellar basal‑body rod protein FlgF
*NZ_CP0092733,498,4470CT46.0% 52.4 / 26.0 50S311S (TCG→TCAyhfSaminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme
*NZ_CP0092734,058,9580GA45.2% 39.5 / 21.4 42L57L (CTG→TTG) yihOMFS transporter
*NZ_CP009273937,1690TG45.2% 36.3 / 13.9 31V252G (GTG→GGG) dmsAdimethylsulfoxide reductase subunit A
*NZ_CP0092735,1590GA44.8% 30.9 / 14.3 29intergenic (+139/‑75)thrC/yaaXthreonine synthase/DUF2502 domain‑containing protein
*NZ_CP0092732,380,3620CT44.4% 14.1 / 10.3 18intergenic (+52/‑51)yfbL/yfbMM28 family metallopeptidase/YfbM family protein
*NZ_CP009273349,9990TG43.8% 142.3 / 41.6 96V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NZ_CP0092734,612,0670AG43.3% 88.5 / 14.6 60E161G (GAG→GGG) yjjJtype II toxin‑antitoxin system HipA family toxin YjjJ
*NZ_CP0092734,213,5250CT42.9% 45.1 / 24.0 42R11K (AGA→AAA) iclRglyoxylate bypass operon transcriptional repressor IclR
*NZ_CP0092734,336,4920TG42.5% 59.3 / 14.2 40T218P (ACC→CCC) fumBclass I fumarate hydratase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 1661537 =30 (0.570)3 (0.060) 3/158 3.5 7.5% intergenic (‑61/+64) bioD/mlc dethiobiotin synthase/DNA‑binding transcriptional repressor Mlc
?NZ_CP009273 3358915 = 45 (0.900)intergenic (+3/+146) BW25113_RS16670/BW25113_RS16680 YhcE family protein/IS5‑like element IS5 family transposase