breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 141 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NZ_CP009273 | 2,141,214 | 0 | T | G | 73.1% | 4.6 / 17.1 | 26 | G20G (GGT→GGG) | yegD | molecular chaperone |
* | NZ_CP009273 | 1,152,727 | 0 | T | G | 66.7% | 9.8 / 24.6 | 27 | G165G (GGT→GGG) | ycfH | metal‑dependent hydrolase |
* | NZ_CP009273 | 997,447 | 0 | T | G | 61.5% | 11.3 / 24.9 | 26 | V62G (GTA→GGA) | elfG | fimbrial protein |
* | NZ_CP009273 | 3,126,661 | 0 | T | G | 60.0% | 13.7 / 31.0 | 25 | H219P (CAC→CCC) | yghS | dTMP kinase |
* | NZ_CP009273 | 353,601 | 0 | C | A | 59.7% | 0.2 / 60.7 | 57 | L267F (TTG→TTT) | cynR | transcriptional regulator CynR |
* | NZ_CP009273 | 760,187 | 0 | G | C | 59.3% | 18.4 / 38.8 | 27 | P184P (CCG→CCC) | sucD | succinate‑‑CoA ligase subunit alpha |
* | NZ_CP009273 | 4,594,161 | 0 | C | T | 57.8% | 11.9 / 77.2 | 66 | S62F (TCT→TTT) | bglJ | DNA‑binding transcriptional activator BglJ |
* | NZ_CP009273 | 760,181 | 0 | G | C | 55.6% | 22.0 / 39.2 | 27 | P182P (CCG→CCC) | sucD | succinate‑‑CoA ligase subunit alpha |
* | NZ_CP009273 | 4,594,167 | 0 | C | T | 53.8% | 22.9 / 84.3 | 66 | S64F (TCT→TTT) | bglJ | DNA‑binding transcriptional activator BglJ |
* | NZ_CP009273 | 1,129,305 | 0 | G | A | 47.1% | 37.9 / 10.5 | 34 | L16L (CTG→CTA) | flgF | flagellar basal‑body rod protein FlgF |
* | NZ_CP009273 | 1,129,313 | 0 | A | G | 46.9% | 29.8 / 11.0 | 32 | Q19R (CAG→CGG) | flgF | flagellar basal‑body rod protein FlgF |
* | NZ_CP009273 | 3,498,447 | 0 | C | T | 46.0% | 52.4 / 26.0 | 50 | S311S (TCG→TCA) | yhfS | aminotransferase class I/II‑fold pyridoxal phosphate‑dependent enzyme |
* | NZ_CP009273 | 4,058,958 | 0 | G | A | 45.2% | 39.5 / 21.4 | 42 | L57L (CTG→TTG) | yihO | MFS transporter |
* | NZ_CP009273 | 937,169 | 0 | T | G | 45.2% | 36.3 / 13.9 | 31 | V252G (GTG→GGG) | dmsA | dimethylsulfoxide reductase subunit A |
* | NZ_CP009273 | 5,159 | 0 | G | A | 44.8% | 30.9 / 14.3 | 29 | intergenic (+139/‑75) | thrC/yaaX | threonine synthase/DUF2502 domain‑containing protein |
* | NZ_CP009273 | 2,380,362 | 0 | C | T | 44.4% | 14.1 / 10.3 | 18 | intergenic (+52/‑51) | yfbL/yfbM | M28 family metallopeptidase/YfbM family protein |
* | NZ_CP009273 | 349,999 | 0 | T | G | 43.8% | 142.3 / 41.6 | 96 | V528G (GTG→GGG) | prpE | propionate‑‑CoA ligase |
* | NZ_CP009273 | 4,612,067 | 0 | A | G | 43.3% | 88.5 / 14.6 | 60 | E161G (GAG→GGG) | yjjJ | type II toxin‑antitoxin system HipA family toxin YjjJ |
* | NZ_CP009273 | 4,213,525 | 0 | C | T | 42.9% | 45.1 / 24.0 | 42 | R11K (AGA→AAA) | iclR | glyoxylate bypass operon transcriptional repressor IclR |
* | NZ_CP009273 | 4,336,492 | 0 | T | G | 42.5% | 59.3 / 14.2 | 40 | T218P (ACC→CCC) | fumB | class I fumarate hydratase |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NZ_CP009273 | 1661537 = | 30 (0.570) | 3 (0.060) | 3/158 | 3.5 | 7.5% | intergenic (‑61/+64) | bioD/mlc | dethiobiotin synthase/DNA‑binding transcriptional repressor Mlc |
? | NZ_CP009273 | 3358915 = | 45 (0.900) | intergenic (+3/+146) | BW25113_RS16670/BW25113_RS16680 | YhcE family protein/IS5‑like element IS5 family transposase |