breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 168,350 | Δ4 bp | coding (867‑870/2244 nt) | fhuA → | ferrichrome outer membrane transporter |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 754,103 | G→T | F122L (TTC→TTA) | gltA ← | citrate synthase |
RA | 1,159,109 | A→G | N414S (AAC→AGC) | ptsG → | fused glucose‑specific PTS enzymes: IIB component/IIC component |
RA | 1,196,220 | C→T | H366H (CAC→CAT) | icd → | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
RA | 1,196,232 | C→T | T370T (ACC→ACT) | icd → | isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase |
JC JC | 1,293,032 | IS1 (–) +8 bp | intergenic (‑110/‑488) | hns ← / → tdk | global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase |
RA | 1,383,685 | C→A | E93* (GAA→TAA) | ycjW ← | LacI family putative transcriptional repressor |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
JC JC | 1,979,486 | IS5 (+) +4 bp | intergenic (‑271/‑264) | insA ← / → uspC | IS1 repressor TnpA/universal stress protein |
RA | 2,022,710 | G→T | G32V (GGC→GTC) | fliQ → | flagellar biosynthesis protein |
RA | 2,132,787 | A→C | I204S (ATC→AGC) | wcaA ← | putative glycosyl transferase |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 2,416,239 | C→A | T498K (ACG→AAG) | pta → | phosphate acetyltransferase |
RA | 2,720,668 | Δ1 bp | coding (716/2661 nt) | pka → | protein lysine acetyltransferase |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 3,799,134 | G→T | S60* (TCG→TAG) | waaU ← | lipopolysaccharide core biosynthesis |
MC JC | 3,815,859 | Δ82 bp | [rph]–[rph] | [rph], [rph] | |
JC | 4,001,645 | Δ5 bp | coding (220‑224/951 nt) | corA → | magnesium/nickel/cobalt transporter |
RA | 4,184,543 | C→A | P1100Q (CCG→CAG) | rpoB → | RNA polymerase, beta subunit |
RA | 4,284,655 | T→A | Y83F (TAT→TTT) | actP ← | acetate transporter |
RA | 4,287,423 | G→C | intergenic (‑52/‑341) | acs ← / → nrfA | acetyl‑CoA synthetase/nitrite reductase, formate‑dependent, cytochrome |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 123033 | 127581 | 4549 | 39 [35] | [37] 38 | [aceE]–[aceF] | [aceE],[aceF] |
* | * | ÷ | NC_000913 | 1196245 | 1211466 | 15222 | 40 [35] | [37] 38 | [icd]–[icdC] | [icd],ymfD,ymfE,lit,intE,xisE,ymfI,ymfJ,cohE,croE,ymfL,ymfM,oweE,aaaE,ymfR,beeE,jayE,ymfQ,stfP,tfaP,tfaE,stfE,pinE,mcrA,[icdC] |
* | * | ÷ | NC_000913 | 3423750–3424234 | 3424534–3424238 | 5–785 | 38 [37] | [37] 39 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 142 (1.590) | 98/276 | 0.0 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |