breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 288,136:1 +A 5.4% coding (1348/1611 nt) yagH → CP4‑6 prophage; putative xylosidase/arabinosidase
RA 288,136:2 +G 5.4% coding (1348/1611 nt) yagH → CP4‑6 prophage; putative xylosidase/arabinosidase
RA 288,136:3 +C 5.4% coding (1348/1611 nt) yagH → CP4‑6 prophage; putative xylosidase/arabinosidase
RA 433,649 T→G 19.2% G65G (GGT→GGG ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
JC JC 458,790 IS186 (–) +6 bp :: Δ1 bp 88.9% intergenic (+90/‑93) clpX → / → lon ATPase and specificity subunit of ClpX‑ClpP ATP‑dependent serine protease/DNA‑binding ATP‑dependent protease La
RA 530,732 A→C 19.2% G165G (GGT→GGG mnmH ← tRNA 2‑selenouridine synthase, selenophosphate‑dependent
RA 540,558 T→G 29.7% intergenic (+50/‑7) allB → / → ybbY allantoinase/putative uracil/xanthine transporter
RA 649,766 T→G 12.4% N242T (AAC→ACC)  citE ← citrate lyase, citryl‑ACP lyase (beta) subunit
RA 1,143,829 A→C 12.4% L180R (CTG→CGG)  rne ← endoribonuclease; RNA‑binding protein;RNA degradosome binding protein
RA 1,196,305 C→T 88.9% R395C (CGT→TGT)  icd → isocitrate dehydrogenase; e14 prophage attachment site; tellurite reductase
JC JC 1,293,032 IS1 (–) +8 bp 100% intergenic (‑110/‑488) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
MC JC 1,299,499 Δ1,199 bp 100% intergenic (+254/‑485) ychE → / → oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
RA 1,466,201 T→C 5.7% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,856 A→G 5.5% pseudogene (1465/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,676,805 T→G 14.3% T367P (ACC→CCC)  pntA ← pyridine nucleotide transhydrogenase, alpha subunit
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,132,787 A→C 100% I204S (ATC→AGC)  wcaA ← putative glycosyl transferase
RA 2,151,689 G→T 31.7% intergenic (+43/+22) yegJ → / ← yegK uncharacterized protein/ser/thr phosphatase‑related protein
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,312,117 A→C 17.8% G211G (GGT→GGG ompC ← outer membrane porin protein C
JC JC 2,536,312 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp 7.0% coding (479‑484/510 nt) crr → glucose‑specific enzyme IIA component of PTS
RA 2,828,506 T→G 18.5% V257G (GTG→GGG)  srlD → sorbitol‑6‑phosphate dehydrogenase
RA 3,206,438 G→A 19.1% intergenic (‑182/‑25) ttdR ← / → ttdA transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
RA 3,206,469 A→G 15.5% S3G (AGC→GGC)  ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,483 G→A 28.1% K7K (AAG→AAA ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,206,506 C→A 14.4% T15K (ACA→AAA)  ttdA → L‑tartrate dehydratase, alpha subunit
RA 3,285,877 C→G 15.6% P134A (CCG→GCG)  agaD → N‑acetylgalactosamine‑specific enzyme IID component of PTS
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,862,661 T→G 42.2% pseudogene (943/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
RA 3,895,556 T→C 15.1% M352V (ATG→GTG)  adeP ← adenine permease, high affinity; adenine:H+ symporter
MC JC 4,001,645 Δ5 bp 100% coding (220‑224/951 nt) corA → magnesium/nickel/cobalt transporter
RA 4,065,596 C→A 16.9% L57L (CTG→CTT yihO ← putative sulphoquinovose importer
RA 4,094,415 T→G 6.2% intergenic (‑143/+308) rhaD ← / ← rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
RA 4,151,329 T→G 16.9% A590A (GCA→GCC ppc ← phosphoenolpyruvate carboxylase
RA 4,151,332 G→C 16.4% G589G (GGC→GGG ppc ← phosphoenolpyruvate carboxylase
JC JC 4,160,853 IS2 (+) +5 bp 11.0% intergenic (‑63/‑267) sthA ← / → fabR pyridine nucleotide transhydrogenase, soluble/transcriptional repressor of fabA and fabB
RA 4,184,543 C→A 100% P1100Q (CCG→CAG)  rpoB → RNA polymerase, beta subunit
RA 4,205,621 G→T 26.0% S104* (TCA→TAA)  purD ← phosphoribosylglycinamide synthetase phosphoribosylamine‑glycine ligase
RA 4,209,827 T→G 13.3% noncoding (54/76 nt) gltV → tRNA‑Glu
RA 4,296,060 C→T 21.1% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +C 93.9% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:2 +G 93.9% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,325,948 A→G 6.3% intergenic (‑207/+451) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,606,902 A→G 14.9% S267P (TCC→CCC)  rsmC ← 16S rRNA m(2)G1207 methyltransferase, SAM‑dependent

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 4233726 4235454 1729 36 [35] [32] 36 pgi pgi

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =33 (0.340)66 (0.720) 47/398 NT 69.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 28 (0.310)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780534 (0.350)77 (0.840) 46/398 NT 72.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 28 (0.310)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 19785020 (0.000)28 (0.300) 24/414 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 2449191 =58 (0.590)3 (0.030) 3/412 NT 5.5% intergenic (+34/+37) smrB/yfcO putative DNA endonuclease/DUF2544 family putative outer membrane protein
?NC_000913 2449213 = 48 (0.510)intergenic (+56/+15) smrB/yfcO putative DNA endonuclease/DUF2544 family putative outer membrane protein
* ? NC_000913 3984220 =NA (NA)3 (0.030) 3/416 NT NA noncoding (1/104 nt) RIP287 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP287 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 3984250 = NA (NA)noncoding (31/104 nt) RIP287 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP287 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 4542682 =28 (0.280)3 (0.030) 3/412 NT 5.4% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542996 = 79 (0.840)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)