breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 26 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0255332,084,5100TA48.2% 28.8 / inf 251intergenic (‑12/+189)dapA/CYL77_099454‑hydroxy‑tetrahydrodipicolinate synthase/thymidylate synthase (FAD)
*CP0255332,084,5080GT48.0% 57.4 / inf 252intergenic (‑10/+191)dapA/CYL77_099454‑hydroxy‑tetrahydrodipicolinate synthase/thymidylate synthase (FAD)
*CP0255332,084,5090GT48.0% 65.4 / inf 252intergenic (‑11/+190)dapA/CYL77_099454‑hydroxy‑tetrahydrodipicolinate synthase/thymidylate synthase (FAD)
*CP0255332,420,7080TC45.9% 284.0 / inf 231P455P (CCT→CCCCYL77_11475HNH endonuclease
*CP0255332,420,7170AC45.8% 331.2 / inf 236G458G (GGA→GGCCYL77_11475HNH endonuclease
*CP0255332,420,7230GA45.6% 285.3 / inf 230V460V (GTG→GTACYL77_11475HNH endonuclease
*CP0255332,420,7100CT45.5% 283.7 / inf 233A456V (GCT→GTT) CYL77_11475HNH endonuclease
*CP0255332,420,7260GT45.2% 334.6 / inf 230G461G (GGG→GGTCYL77_11475HNH endonuclease
*CP0255332,420,7330CT44.9% 293.7 / inf 225P464S (CCA→TCA) CYL77_11475HNH endonuclease
*CP0255332,420,7370TC43.4% 306.4 / 315.3 219V465A (GTG→GCG) ‡CYL77_11475HNH endonuclease
*CP0255332,420,7380GA43.4% 314.5 / inf 219V465V (GTG→GTA) ‡CYL77_11475HNH endonuclease
*CP0255332,420,7390GA43.4% 316.2 / inf 219G466S (GGT→AGT) CYL77_11475HNH endonuclease
*CP0255332,086,2440TG42.6% 149.5 / inf 256G2G (GGA→GGCCYL77_099504‑hydroxy‑tetrahydrodipicolinate reductase
*CP0255332,420,7810AC36.4% 437.5 / 311.4 209S480R (AGT→CGT) CYL77_11475HNH endonuclease
*CP025533985,6470AC36.0% 274.7 / 239.7 164P272P (CCA→CCCCYL77_04730HNH endonuclease
*CP0255332,420,8100AG35.4% 407.9 / 237.9 206K489K (AAA→AAGCYL77_11475HNH endonuclease
*CP0255332,420,7860GC35.3% 474.5 / 277.4 207S481S (TCG→TCCCYL77_11475HNH endonuclease
*CP0255332,420,8280CG34.9% 483.7 / 287.0 210P495P (CCC→CCGCYL77_11475HNH endonuclease
*CP025533985,6710AC32.9% 320.6 / 218.1 167R280R (CGA→CGCCYL77_04730HNH endonuclease
*CP0255331,177,1200TG30.2% 246.8 / 18.4 107N259T (AAC→ACC) kgd2‑oxoglutarate dehydrogenase E1/E2 component

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP025533 = 497710105 (0.830)6 (0.070) 6/346 15.8 7.2% intergenic (+2/‑251) CYL77_02460/CYL77_02465 tRNA‑Thr/tRNA‑Thr
?CP025533 498036 = 82 (0.930)intergenic (+3/‑36) CYL77_02465/CYL77_02470 tRNA‑Thr/tRNA‑Met