breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 84 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM11,327,1210CT51.8% 785.6 / inf 1705intergenic (+97/‑196)PP_16SE rRNA (16S ribosomal RNA)/PP_23SE rRNA (23S ribosomal RNA)–/–
*AE015451_phaM14,348,7050AG49.5% 99.0 / inf 1449intergenic (‑70/+404)PP_3820 CDS (Group II intron‑encoding maturase)/galU CDS (UTP‑glucose‑1‑phosphate uridylyltransferase)–/–
*AE015451_phaM1699,7220AG47.9% 75.6 / inf 999intergenic (+115/‑129)PP_t06 tRNA (tRNA‑Ala)/PP_23SD rRNA (23S ribosomal RNA)–/–
*AE015451_phaM15,223,0130CA42.9% ‑4.9 / 24.1 42L428F (TTG→TTTPP_4603 CDS (transposase)
*AE015451_phaM14,344,3120GA42.5% 23.9 / 49.7 106intergenic (+43/‑113)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
*AE015451_phaM14,889,6230AG40.7% 157.8 / 39.5 141intergenic (+69/‑84)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
*AE015451_phaM14,675,3600TG36.5% 221.2 / 57.5 206intergenic (+59/+45)PP_4137 CDS (putative Outer membrane siderophore receptor)/chrR CDS (Chromate reductase)–/–
*AE015451_phaM1195,6340CT36.3% 1656.5 / inf 1289T380T (ACC→ACTPP_0168 CDS (putative surface adhesion protein)
*AE015451_phaM15,737,7560AG36.1% 295.5 / 86.2 260intergenic (+45/+26)hutF CDS (probable formiminoglutamate deiminase)/PP_5037 CDS (lipocalin family lipoprotein)–/–
*AE015451_phaM14,344,3080GA34.5% 125.3 / 23.7 123intergenic (+39/‑117)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
*AE015451_phaM12,178,5680GA33.1% 2226.2 / inf 1296T268T (ACG→ACAPP_1931 CDS (conserved protein of unknown function)
*AE015451_phaM14,889,6440GC32.6% 481.1 / 134.0 266intergenic (+90/‑63)hyi CDS (hydroxypyruvate isomerase)/glxR CDS (tartronate semialdehyde reductase)–/–
*AE015451_phaM15,199,6560GT32.3% 87.0 / 40.8 133intergenic (+31/+42)PP_4579 CDS (Transcriptional regulator_ LysR family)/PP_4580 CDS (conserved protein of unknown function)–/–
*AE015451_phaM1445,1120CT31.8% 167.1 / 11.9 89intergenic (+85/‑203)PP_0367 CDS (conserved protein of unknown function)/PP_0368 CDS (putative Acyl‑CoA dehydrogenase)–/–
*AE015451_phaM15,485,1530AG31.7% 136.5 / 14.7 88intergenic (+59/‑88)purH CDS (phosphoribosylaminoimidazolecarboxamide formyltransferase_IMP cyclohydrolase)/purD CDS (Phosphoribosylamine‑‑glycine ligase)–/–
*AE015451_phaM15,199,6570CT29.9% 94.8 / 28.1 132intergenic (+32/+41)PP_4579 CDS (Transcriptional regulator_ LysR family)/PP_4580 CDS (conserved protein of unknown function)–/–
*AE015451_phaM14,344,3230GA29.1% 242.3 / 17.5 147intergenic (+54/‑102)PP_3817 CDS (Polyamine ABC transporter_ ATP‑binding protein)/PP_3818 CDS (Periplamic phosphate‑binding protein)–/–
*AE015451_phaM15,814,1780CT28.2% 409.2 / 47.2 223intergenic (+88/+41)PP_5092 CDS (putative exported NLP_P60 protein)/pilT CDS (twitching motility protein)–/–
*AE015451_phaM15,344,4860TG27.8% 434.6 / 63.7 287intergenic (+61/‑117)pgi‑II CDS (glucose‑6‑phosphate isomerase)/acsA‑II CDS (acetyl‑CoA synthetase)–/–
*AE015451_phaM11,210,8390GA27.3% 1078.5 / 61.1 502intergenic (+29/+8)ytnA CDS (Uncharacterized amino acid permease YtnA)/PP_1060 CDS (putative Glutamate synthase_ large subunit)–/–

Marginal new junction evidence (lowest skew 10 of 232 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AE015451_phaM1 = 555560834 (0.040)301 (0.360)
+GCC
56/264 4.7 90.0% intergenic (‑84/+87) PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer) –/–
?AE015451_phaM1 5555609 = 34 (0.040)intergenic (‑85/+86) PP_4887 CDS (conserved protein of unknown function)/PP_4888 CDS (putative methyl‑accepting chemotaxis transducer) –/–
* ? AE015451_phaM1 4808174 =57 (0.070)251 (0.400) 32/200 6.4 53.7% intergenic (‑283/‑272) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
?AE015451_phaM1 = 5986211 391 (0.620)intergenic (+229/‑317) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–
* ? AE015451_phaM1 = 56814150 (0.000)237 (0.290)
+CGGG
43/262 6.7 100% intergenic (‑133/+71) PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein) –/–
?AE015451_phaM1 5681416 = 0 (0.000)intergenic (‑134/+70) PP_4986 CDS (Channel protein_ hemolysin III family)/PP_4987 CDS (putative Chemotaxis protein) –/–
* ? AE015451_phaM1 = 652782720 (0.840)136 (0.190) 33/230 7.5 30.7% coding (1961/1962 nt) ctpL CDS (Methyl‑accepting chemotaxis protein CtpL)
?AE015451_phaM1 = 4808159 0 (0.000)intergenic (‑268/‑287) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
* ? AE015451_phaM1 = 2472115215 (0.250)81 (0.100) 36/256 7.9 20.5% intergenic (+19/+130) PP_2166 CDS (Anti‑anti‑sigma factor)/PP_2167 CDS (putative PhoD‑like phosphatase) –/–
?AE015451_phaM1 2472313 = 424 (0.520)coding (1783/1851 nt) PP_2167 CDS (putative PhoD‑like phosphatase)
* ? AE015451_phaM1 = 480817910 (0.010)121 (0.160) 29/246 9.2 30.8% intergenic (‑288/‑267) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
?AE015451_phaM1 5986041 = 536 (0.690)intergenic (+59/‑487) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–
* ? AE015451_phaM1 = 480817910 (0.010)114 (0.160) 25/230 9.6 32.6% intergenic (‑288/‑267) PP_4234 CDS (Aldehyde oxidase and xanthine dehydrogenase)/dsbD‑II CDS (thiol_disulfide interchange protein 2) –/–
?AE015451_phaM1 = 5986225 463 (0.630)intergenic (+243/‑303) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–
* ? AE015451_phaM1 = 2282748500 (0.580)48 (0.060) 27/258 10.2 8.9% intergenic (‑140/+89) PP_2010 CDS (Cytochrome b561)/PP_2011 CDS (conserved exported protein of unknown function) –/–
?AE015451_phaM1 2282815 = 500 (0.610)intergenic (‑207/+22) PP_2010 CDS (Cytochrome b561)/PP_2011 CDS (conserved exported protein of unknown function) –/–
* ? AE015451_phaM1 = 5986033573 (0.670)39 (0.050) 26/254 10.4 7.0% intergenic (+51/‑495) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–
?AE015451_phaM1 5986255 = 491 (0.610)intergenic (+273/‑273) kefB‑III CDS (glutathione‑regulated potassium_H+ antiporter)/PP_mr64 ncRNA (C4 ncRNA) –/–
* ? AE015451_phaM1 = 5201567322 (0.380)31 (0.040) 24/264 11.4 11.6% intergenic (‑53/‑127) PP_4582 CDS (conserved membrane protein of unknown function)/PP_4583 CDS (putative Peptidase) –/–
?AE015451_phaM1 5201666 = 157 (0.190)intergenic (‑152/‑28) PP_4582 CDS (conserved membrane protein of unknown function)/PP_4583 CDS (putative Peptidase) –/–