breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 53 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0349432,981,2530CT46.7% ‑6.0 / 18.4 15noncoding (1267/1550 nt)EO946_RS1543516S ribosomal RNA
*NZ_CP0349433,285,5800AG42.8% 285.1 / inf 382I274T (ATA→ACA) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,6470CA42.4% 301.9 / inf 434V252L (GTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349432,991,2360CG40.0% 218.4 / 83.8 181intergenic (+37/‑170)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349433,285,5750TG37.9% 389.4 / inf 377N276H (AAT→CAT) EO946_RS16995IS3 family transposase
*NZ_CP0349433,285,5670CT37.7% 366.1 / inf 371L278L (CTG→CTAEO946_RS16995IS3 family transposase
*NZ_CP034943634,0540CG33.7% 271.7 / 49.8 174E573Q (GAA→CAA) EO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP0349433,285,7130GA30.1% 381.4 / 200.7 221L230L (CTA→TTA) EO946_RS16995IS3 family transposase
*NZ_CP0349431,730,0960GC30.1% 207.3 / 30.3 137E173Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP0349432,270,0640GC29.5% 296.3 / 39.9 186L39V (CTA→GTA) prsWglutamic‑type intramembrane protease PrsW
*NZ_CP0349432,830,5230CG27.9% 371.9 / 43.0 210L89V (CTT→GTT) brnQbranched‑chain amino acid transport system II carrier protein
*NZ_CP0349432,991,2270TG27.8% 268.4 / 49.5 187intergenic (+28/‑179)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349432,991,2520CA27.5% 297.6 / 18.3 174intergenic (+53/‑154)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP0349433,219,7970GT27.0% 269.2 / 34.7 166V379L (GTA→TTA) oxdCoxalate decarboxylase
*NZ_CP0349432,270,0520GC26.7% 364.4 / 36.1 201L43V (CTT→GTT) prsWglutamic‑type intramembrane protease PrsW
*NZ_CP034943634,0580CG26.5% 287.8 / 29.5 174K571N (AAG→AACEO946_RS03330ABC‑F family ATP‑binding cassette domain‑containing protein
*NZ_CP0349432,991,2450GT25.8% 286.3 / 38.2 182intergenic (+46/‑161)gbsR/bslAtranscriptional regulator GbsR/biofilm‑surface layer protein BslA
*NZ_CP034943595,2030CA25.8% 369.7 / 49.0 199T30K (ACG→AAG) EO946_RS03130MMPL family transporter
*NZ_CP0349431,730,0750GC25.7% 278.1 / 22.3 147E166Q (GAA→CAA) rodZcell shape determination protein RodZ
*NZ_CP0349433,446,9700GT25.5% 396.1 / 69.7 239Q206K (CAA→AAA) degUtwo‑component system response regulator DegU

Marginal new junction evidence (lowest skew 10 of 11 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP034943 = 607243373 (1.480)27 (0.110) 14/254 6.5 8.5% coding (86/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607703 = 231 (0.980)coding (546/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607243373 (1.480)15 (0.060) 8/254 8.6 5.2% coding (86/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607631 = 195 (0.820)coding (474/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607225416 (1.650)16 (0.060) 8/266 8.9 4.6% coding (68/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607442 = 255 (1.030)coding (285/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 607443 =270 (1.070)11 (0.050)
+AGTAGCCCCTGTGGCTCCC
6/232 9.0 3.9% coding (286/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607837 367 (1.460)coding (680/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607243377 (1.500)23 (0.100)
+CGGACCAG
7/254 9.1 7.7% coding (86/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607675 = 208 (0.830)coding (518/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 607695 =230 (0.910)10 (0.040)
+AGTAGCCCCT
6/250 9.5 3.6% coding (538/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 = 607846 342 (1.360)coding (689/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607429256 (1.020)4 (0.020) 4/216 9.7 2.1% coding (272/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607691 = 167 (0.830)coding (534/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 = 607682222 (0.900)8 (0.030)
+ACGGGAGCC
5/248 10.0 3.7% coding (525/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607683 = 224 (0.890)coding (526/1125 nt) EO946_RS03185 collagen‑like protein
* ? NZ_CP034943 767776 =291 (1.160)4 (0.020) 3/244 11.2 1.5% coding (189/645 nt) EO946_RS03930 hypothetical protein
?NZ_CP034943 = 767772 278 (1.220)coding (193/645 nt) EO946_RS03930 hypothetical protein
* ? NZ_CP034943 = 607646214 (0.860)3 (0.010)
+ACGGGAGCC
3/248 11.3 1.4% coding (489/1125 nt) EO946_RS03185 collagen‑like protein
?NZ_CP034943 607683 = 227 (0.900)coding (526/1125 nt) EO946_RS03185 collagen‑like protein