breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 36 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG40.7% 372.8 / 49.1 221intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP012306935,0540GT33.8% 138.0 / 46.2 74intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123063,020,6700TG33.3% 365.8 / 11.6 195K195N (AAA→AACglgCglucose‑1‑phosphate adenylyltransferase
*AP0123061,944,2880AC32.0% 491.7 / 44.0 234G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123063,938,8870CT30.8% 424.5 / 134.1 227S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP012306644,4170GA30.4% 891.0 / inf 569intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123061,854,8510AC29.9% 570.6 / 70.6 251T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123061,128,4120TG27.3% 797.9 / 47.3 326V29G (GTG→GGG) yciOconserved protein
*AP0123062,360,0480TG27.2% 473.4 / 11.7 191T113P (ACC→CCC) hycBhydrogenase 3, Fe‑S subunit
*AP0123063,903,7910AG26.9% 563.7 / 79.7 260E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123063,372,7010TG26.6% 132.3 / 12.1 64noncoding (54/76 nt)gltUtRNA‑Glu
*AP0123063,639,0400TG25.9% 121.5 / 11.1 58noncoding (54/76 nt)gltVtRNA‑Glu
*AP012306472,8680TG25.6% 552.7 / 13.1 203D435A (GAC→GCC) cusSsensory histidine kinase in two‑component regulatory system with CusR, senses copper ions
*AP012306202,4170TG25.6% 568.6 / 41.4 254V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP012306800,6750AC25.0% 645.2 / 26.9 264T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP012306524,2970CT23.6% 354.5 / 43.7 144M1M (ATG→ATA) †citDcitrate lyase, acyl carrier (gamma) subunit
*AP0123061,226,5570AG23.4% 387.0 / 64.9 171G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123062,560,2430TG23.2% 476.8 / 23.5 190N419T (AAC→ACC) pepPproline aminopeptidase P II
*AP0123062,558,0710AC22.9% 589.4 / 30.3 231V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
*AP0123062,680,3450AC22.9% 538.6 / 28.6 228T184P (ACC→CCC) ttdAL‑tartrate dehydratase, alpha subunit

Marginal new junction evidence (lowest skew 10 of 140 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 1092512368 (1.200)19 (0.070) 12/258 9.8 6.0% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 280 (1.060)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 997497298 (0.980)13 (0.050) 12/268 10.0 4.7% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 254 (0.930)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 2684153354 (1.160)8 (0.030) 8/288 11.5 2.3% coding (40/216 nt) rpsU 30S ribosomal subunit protein S21
?AP012306 = 2684161 344 (1.170)coding (48/216 nt) rpsU 30S ribosomal subunit protein S21
* ? AP012306 = 2275012269 (0.880)7 (0.020) 7/274 11.7 2.8% noncoding (60/76 nt) gltW tRNA‑Glu
?AP012306 3372719 = NA (NA)noncoding (72/76 nt) gltU tRNA‑Glu
* ? AP012306 = 1883833409 (1.340)7 (0.020) 7/274 11.7 1.9% intergenic (‑717/‑22) ompC/rcsD outer membrane porin protein C/phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
?AP012306 = 1883821 363 (1.290)intergenic (‑705/‑34) ompC/rcsD outer membrane porin protein C/phosphotransfer intermediate protein in two‑component regulatory system with RcsBC
* ? AP012306 = 1092334281 (0.920)7 (0.030) 6/258 11.8 2.6% coding (198/276 nt) ychS predicted protein
?AP012306 1092985 = 280 (1.060)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 1328974 =302 (0.990)7 (0.020) 7/290 11.9 2.2% coding (816/1188 nt) ydeE predicted transporter
?AP012306 1328990 = 316 (1.060)coding (832/1188 nt) ydeE predicted transporter
* ? AP012306 = 1178769274 (0.900)6 (0.020) 6/274 12.0 2.3% coding (451/843 nt) ycjP predicted sugar transporter subunit
?AP012306 = 1178757 253 (0.900)coding (439/843 nt) ycjP predicted sugar transporter subunit
* ? AP012306 228777 =369 (1.210)6 (0.020) 6/278 12.1 1.7% coding (275/771 nt) yafV predicted C‑N hydrolase family amidase, NAD(P)‑binding
?AP012306 228787 = 358 (1.260)coding (265/771 nt) yafV predicted C‑N hydrolase family amidase, NAD(P)‑binding
* ? AP012306 1108421 =220 (0.720)6 (0.020) 6/284 12.2 2.6% coding (359/909 nt) oppC oligopeptide transporter subunit
?AP012306 1108462 = 239 (0.820)coding (400/909 nt) oppC oligopeptide transporter subunit