breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 33 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG42.4% 495.5 / 71.5 290intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC38.6% 404.6 / 54.0 228G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123061,226,5570AG31.5% 327.4 / 101.5 181G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP012306644,4170GA29.5% 943.8 / inf 576intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123063,527,3680AC28.8% 628.4 / 58.1 278L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP012306202,4170TG28.7% 604.6 / 47.5 289V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP0123061,421,1470TG27.5% 424.9 / 14.0 220T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
*AP0123063,938,8870CT26.8% 566.0 / 152.5 272S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123061,267,5780TG26.5% 143.0 / 17.3 68D60A (GAC→GCC) ddpBD‑Ala‑D‑Ala transporter subunit
*AP0123063,282,0760TG26.4% 557.2 / 17.1 252intergenic (‑79/+27)ilvB/ivbLacetolactate synthase I, large subunit/ilvB operon leader peptide
*AP012306341,3770TG25.4% 230.0 / 12.3 123G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP012306800,6750AC25.1% 692.6 / 36.4 280T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP012306935,0540GT24.7% 237.9 / 41.7 89intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP0123062,758,9090TG24.3% 585.8 / 20.1 230G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123062,212,7530AC24.0% 376.7 / 34.8 154Y241D (TAT→GAT) hcaTpredicted 3‑phenylpropionic transporter
*AP0123062,680,3450AC23.9% 416.5 / 26.1 188T184P (ACC→CCC) ttdAL‑tartrate dehydratase, alpha subunit
*AP0123062,714,5550TG23.1% 705.1 / 11.8 285T175P (ACC→CCC) ygjVconserved inner membrane protein
*AP0123061,128,4120TG22.9% 802.5 / 37.0 306V29G (GTG→GGG) yciOconserved protein
*AP0123063,182,8610AC22.6% 388.0 / 10.9 146L198F (TTA→TTCyiaNpredicted transporter
*AP0123063,618,8420AG22.5% 927.8 / 51.3 360intergenic (+56/‑157)rpoC/htrCRNA polymerase, beta prime subunit/heat shock protein

Marginal new junction evidence (lowest skew 10 of 393 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 644310 =359 (1.100)86 (0.270)
+GAAG
13/290 10.7 20.0% noncoding (1/76 nt) lysQ tRNA‑Lys
?AP012306 = 2073420 349 (1.070)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 = 1092512396 (1.220)13 (0.050) 10/258 11.0 3.9% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 305 (1.080)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 = 997497306 (0.940)9 (0.030) 9/268 11.5 3.2% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 262 (0.900)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 214142NA (NA)10 (0.030) 10/288 11.5 NA noncoding (1511/1542 nt) rrsH 16S ribosomal RNA (rrsH)
?AP012306 = 214154 NA (NA)noncoding (1523/1542 nt) rrsH 16S ribosomal RNA (rrsH)
* ? AP012306 1865265 =159 (0.490)9 (0.030) 9/274 11.6 5.7% coding (7/1944 nt) ccmF heme lyase, CcmF subunit
?AP012306 1865277 = 149 (0.500)coding (471/480 nt) ccmE periplasmic heme chaperone
* ? AP012306 = 1728858349 (1.070)9 (0.030) 9/274 11.6 2.7% coding (298/642 nt) udk uridine/cytidine kinase
?AP012306 = 1728846 316 (1.060)coding (310/642 nt) udk uridine/cytidine kinase
* ? AP012306 = 217413NA (NA)22 (0.070)
+CCTTAAAGG
9/280 11.7 NA noncoding (2794/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 217413 NA (NA)noncoding (2794/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 3600658NA (NA)25 (0.080) 9/292 11.8 NA noncoding (2805/2904 nt) rrlB 23S ribosomal RNA (rrlB)
?AP012306 = 3642045 NA (NA)noncoding (2790/2904 nt) rrlE 23S ribosomal RNA (rrlE)
* ? AP012306 = 3800268403 (1.240)8 (0.030) 8/274 11.9 2.1% coding (142/1647 nt) groL Cpn60 chaperonin GroEL, large subunit of GroESL
?AP012306 = 3800256 375 (1.250)coding (130/1647 nt) groL Cpn60 chaperonin GroEL, large subunit of GroESL
* ? AP012306 = 3331682331 (1.020)10 (0.030) 9/294 11.9 2.9% coding (1233/1413 nt) bglB cryptic phospho‑beta‑glucosidase B
?AP012306 = 3331695 341 (1.060)coding (1220/1413 nt) bglB cryptic phospho‑beta‑glucosidase B