breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 28 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | AP012306 | 1,091,992 | 0 | T | G | 38.3% | 472.3 / 75.2 | 274 | intergenic (+38/‑145) | narI/ychS | nitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein |
* | AP012306 | 644,417 | 0 | G | A | 32.5% | 818.9 / inf | 590 | intergenic (+32/‑401) | lysQ/nadA | tRNA‑Lys/quinolinate synthase, subunit A |
* | AP012306 | 341,377 | 0 | T | G | 30.9% | 185.1 / 24.5 | 123 | G60G (GGT→GGG) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase |
* | AP012306 | 3,938,887 | 0 | C | T | 30.1% | 487.9 / 159.5 | 261 | S44F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator |
* | AP012306 | 1,944,288 | 0 | A | C | 28.8% | 509.8 / 37.1 | 229 | G361G (GGT→GGG) | menE | o‑succinylbenzoate‑CoA ligase |
* | AP012306 | 3,182,861 | 0 | A | C | 26.3% | 268.7 / 22.5 | 114 | L198F (TTA→TTC) | yiaN | predicted transporter |
* | AP012306 | 3,527,368 | 0 | A | C | 25.7% | 557.9 / 37.2 | 229 | L140F (TTA→TTC) | rhaS | DNA‑binding transcriptional activator, L‑rhamnose‑binding |
* | AP012306 | 1,854,851 | 0 | A | C | 24.9% | 591.2 / 52.9 | 242 | T25P (ACC→CCC) | yejH | predicted ATP‑dependet helicase |
* | AP012306 | 2,714,555 | 0 | T | G | 24.5% | 648.4 / 14.4 | 266 | T175P (ACC→CCC) | ygjV | conserved inner membrane protein |
* | AP012306 | 3,590,367 | 0 | A | C | 24.5% | 626.4 / 40.6 | 245 | T30P (ACC→CCC) | fabR | DNA‑binding transcriptional repressor |
* | AP012306 | 800,675 | 0 | A | C | 24.2% | 629.5 / 34.2 | 256 | T192P (ACC→CCC) | lolA | chaperone for lipoproteins |
* | AP012306 | 3,412,049 | 0 | T | G | 23.4% | 718.6 / 42.3 | 296 | intergenic (+25/‑122) | proM/aslB | tRNA‑Pro/predicted regulator of arylsulfatase activity |
* | AP012306 | 1,226,557 | 0 | A | G | 23.0% | 464.0 / 60.5 | 191 | G55G (GGA→GGG) | ydcU | predicted spermidine/putrescine transporter subunit |
* | AP012306 | 3,903,791 | 0 | A | G | 22.8% | 640.1 / 60.7 | 268 | E189G (GAA→GGA) | yjgJ | predicted transcriptional regulator |
* | AP012306 | 1,066,323 | 0 | A | C | 22.1% | 675.2 / 15.5 | 240 | intergenic (‑92/+33) | ychM/prsA | predicted transporter/phosphoribosylpyrophosphate synthase |
* | AP012306 | 3,456,453 | 0 | A | C | 22.1% | 671.8 / 11.3 | 240 | intergenic (+12/+369) | fre/fadA | flavin reductase/3‑ketoacyl‑CoA thiolase |
* | AP012306 | 121,217 | 0 | C | A | 21.7% | 691.8 / 77.0 | 258 | A233D (GCC→GAC) | aceF | pyruvate dehydrogenase, dihydrolipoyltransacetylase component E2 |
* | AP012306 | 1,128,412 | 0 | T | G | 21.3% | 722.1 / 31.1 | 269 | V29G (GTG→GGG) | yciO | conserved protein |
* | AP012306 | 2,560,243 | 0 | T | G | 21.0% | 530.1 / 19.7 | 206 | N419T (AAC→ACC) | pepP | proline aminopeptidase P II |
* | AP012306 | 202,417 | 0 | T | G | 20.8% | 704.2 / 14.1 | 276 | V409G (GTA→GGA) | tilS | tRNA(Ile)‑lysidine synthetase |
Marginal new junction evidence (lowest skew 10 of 316 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | AP012306 | 644106 = | 437 (1.400) | 22 (0.070) +CCCGGG |
11/286 | 15.3 | 5.5% | noncoding (5/76 nt) | lysZ | tRNA‑Lys |
? | AP012306 | = 2073420 | 350 (1.120) | noncoding (76/76 nt) | valU | tRNA‑Val | |||||
* | ? | AP012306 | 1856463 = | 246 (0.790) | 9 (0.030) | 9/274 | 15.8 | 3.9% | coding (1685/1761 nt) | yejH | predicted ATP‑dependet helicase |
? | AP012306 | 1856475 = | 222 (0.780) | coding (1697/1761 nt) | yejH | predicted ATP‑dependet helicase | |||||
* | ? | AP012306 | 1083750 = | 256 (0.820) | 10 (0.030) | 10/290 | 15.8 | 3.8% | coding (366/1392 nt) | narK | nitrate/nitrite transporter |
? | AP012306 | 1083769 = | 263 (0.870) | coding (385/1392 nt) | narK | nitrate/nitrite transporter | |||||
* | ? | AP012306 | = 548988 | 408 (1.310) | 9 (0.030) | 9/274 | 15.8 | 2.4% | coding (455/483 nt) | ybeL | conserved protein |
? | AP012306 | = 548976 | 372 (1.300) | coding (443/483 nt) | ybeL | conserved protein | |||||
* | ? | AP012306 | 644314 = | 356 (1.140) | 22 (0.070) +CCCGGG |
9/286 | 16.1 | 6.1% | noncoding (5/76 nt) | lysQ | tRNA‑Lys |
? | AP012306 | = 2073420 | 350 (1.120) | noncoding (76/76 nt) | valU | tRNA‑Val | |||||
* | ? | AP012306 | = 1980895 | 248 (0.800) | 9 (0.030) | 9/292 | 16.3 | 3.6% | coding (825/1833 nt) | yfbS | predicted transporter |
? | AP012306 | = 1980905 | 244 (0.800) | coding (815/1833 nt) | yfbS | predicted transporter | |||||
* | ? | AP012306 | 228777 = | 325 (1.040) | 8 (0.030) | 8/278 | 16.4 | 2.5% | coding (275/771 nt) | yafV | predicted C‑N hydrolase family amidase, NAD(P)‑binding |
? | AP012306 | 228787 = | 309 (1.060) | coding (265/771 nt) | yafV | predicted C‑N hydrolase family amidase, NAD(P)‑binding | |||||
* | ? | AP012306 | = 3608580 | 364 (1.170) | 8 (0.030) | 8/284 | 16.5 | 2.3% | coding (489/705 nt) | rplA | 50S ribosomal subunit protein L1 |
? | AP012306 | = 3608581 | 333 (1.120) | coding (490/705 nt) | rplA | 50S ribosomal subunit protein L1 | |||||
* | ? | AP012306 | = 3938663 | 463 (1.490) | 8 (0.030) | 8/288 | 16.6 | 1.7% | coding (644/726 nt) | yjjQ | predicted DNA‑binding transcriptional regulator |
? | AP012306 | = 3938672 | 456 (1.510) | coding (653/726 nt) | yjjQ | predicted DNA‑binding transcriptional regulator | |||||
* | ? | AP012306 | 2780478 = | 290 (0.930) | 10 (0.030) | 8/288 | 16.6 | 3.4% | coding (716/1890 nt) | deaD | ATP‑dependent RNA helicase |
? | AP012306 | 2780499 = | 281 (0.930) | coding (695/1890 nt) | deaD | ATP‑dependent RNA helicase |