breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 28 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG38.3% 472.3 / 75.2 274intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP012306644,4170GA32.5% 818.9 / inf 590intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP012306341,3770TG30.9% 185.1 / 24.5 123G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123063,938,8870CT30.1% 487.9 / 159.5 261S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123061,944,2880AC28.8% 509.8 / 37.1 229G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123063,182,8610AC26.3% 268.7 / 22.5 114L198F (TTA→TTCyiaNpredicted transporter
*AP0123063,527,3680AC25.7% 557.9 / 37.2 229L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123061,854,8510AC24.9% 591.2 / 52.9 242T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123062,714,5550TG24.5% 648.4 / 14.4 266T175P (ACC→CCC) ygjVconserved inner membrane protein
*AP0123063,590,3670AC24.5% 626.4 / 40.6 245T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor
*AP012306800,6750AC24.2% 629.5 / 34.2 256T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123063,412,0490TG23.4% 718.6 / 42.3 296intergenic (+25/‑122)proM/aslBtRNA‑Pro/predicted regulator of arylsulfatase activity
*AP0123061,226,5570AG23.0% 464.0 / 60.5 191G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123063,903,7910AG22.8% 640.1 / 60.7 268E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123061,066,3230AC22.1% 675.2 / 15.5 240intergenic (‑92/+33)ychM/prsApredicted transporter/phosphoribosylpyrophosphate synthase
*AP0123063,456,4530AC22.1% 671.8 / 11.3 240intergenic (+12/+369)fre/fadAflavin reductase/3‑ketoacyl‑CoA thiolase
*AP012306121,2170CA21.7% 691.8 / 77.0 258A233D (GCC→GAC) aceFpyruvate dehydrogenase, dihydrolipoyltransacetylase component E2
*AP0123061,128,4120TG21.3% 722.1 / 31.1 269V29G (GTG→GGG) yciOconserved protein
*AP0123062,560,2430TG21.0% 530.1 / 19.7 206N419T (AAC→ACC) pepPproline aminopeptidase P II
*AP012306202,4170TG20.8% 704.2 / 14.1 276V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase

Marginal new junction evidence (lowest skew 10 of 316 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 644106 =437 (1.400)22 (0.070)
+CCCGGG
11/286 15.3 5.5% noncoding (5/76 nt) lysZ tRNA‑Lys
?AP012306 = 2073420 350 (1.120)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 1856463 =246 (0.790)9 (0.030) 9/274 15.8 3.9% coding (1685/1761 nt) yejH predicted ATP‑dependet helicase
?AP012306 1856475 = 222 (0.780)coding (1697/1761 nt) yejH predicted ATP‑dependet helicase
* ? AP012306 1083750 =256 (0.820)10 (0.030) 10/290 15.8 3.8% coding (366/1392 nt) narK nitrate/nitrite transporter
?AP012306 1083769 = 263 (0.870)coding (385/1392 nt) narK nitrate/nitrite transporter
* ? AP012306 = 548988408 (1.310)9 (0.030) 9/274 15.8 2.4% coding (455/483 nt) ybeL conserved protein
?AP012306 = 548976 372 (1.300)coding (443/483 nt) ybeL conserved protein
* ? AP012306 644314 =356 (1.140)22 (0.070)
+CCCGGG
9/286 16.1 6.1% noncoding (5/76 nt) lysQ tRNA‑Lys
?AP012306 = 2073420 350 (1.120)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 = 1980895248 (0.800)9 (0.030) 9/292 16.3 3.6% coding (825/1833 nt) yfbS predicted transporter
?AP012306 = 1980905 244 (0.800)coding (815/1833 nt) yfbS predicted transporter
* ? AP012306 228777 =325 (1.040)8 (0.030) 8/278 16.4 2.5% coding (275/771 nt) yafV predicted C‑N hydrolase family amidase, NAD(P)‑binding
?AP012306 228787 = 309 (1.060)coding (265/771 nt) yafV predicted C‑N hydrolase family amidase, NAD(P)‑binding
* ? AP012306 = 3608580364 (1.170)8 (0.030) 8/284 16.5 2.3% coding (489/705 nt) rplA 50S ribosomal subunit protein L1
?AP012306 = 3608581 333 (1.120)coding (490/705 nt) rplA 50S ribosomal subunit protein L1
* ? AP012306 = 3938663463 (1.490)8 (0.030) 8/288 16.6 1.7% coding (644/726 nt) yjjQ predicted DNA‑binding transcriptional regulator
?AP012306 = 3938672 456 (1.510)coding (653/726 nt) yjjQ predicted DNA‑binding transcriptional regulator
* ? AP012306 2780478 =290 (0.930)10 (0.030) 8/288 16.6 3.4% coding (716/1890 nt) deaD ATP‑dependent RNA helicase
?AP012306 2780499 = 281 (0.930)coding (695/1890 nt) deaD ATP‑dependent RNA helicase