breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 34 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG36.4% 630.9 / 70.5 319intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,421,1470TG31.6% 363.5 / 15.0 196T79P (ACC→CCC) ydhBpredicted DNA‑binding transcriptional regulator
*AP012306341,3770TG31.6% 251.5 / 13.6 136G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP012306644,4170GA30.4% 778.4 / inf 497intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123063,527,3680AC29.4% 569.2 / 62.8 248L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP0123063,938,8870CT28.9% 461.8 / 147.3 235S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123063,182,8610AC28.1% 293.5 / 26.4 128L198F (TTA→TTCyiaNpredicted transporter
*AP0123061,944,2880AC27.2% 560.9 / 31.2 229G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123062,758,9090TG26.8% 527.9 / 16.8 205G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP012306800,6750AC26.8% 671.2 / 31.9 280T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP0123062,558,0710AC26.3% 620.8 / 37.2 255V302G (GTA→GGA) visCpredicted oxidoreductase, FAD/NAD(P)‑binding domain
*AP0123063,412,0490TG26.0% 816.3 / 39.2 339intergenic (+25/‑122)proM/aslBtRNA‑Pro/predicted regulator of arylsulfatase activity
*AP0123061,226,5570AG25.3% 421.7 / 73.5 186G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP0123061,128,4120TG24.5% 747.4 / 54.0 296V29G (GTG→GGG) yciOconserved protein
*AP0123061,270,2260AG24.3% 463.6 / 37.6 177S779P (TCC→CCC) doscAMP phosphodiesterase, heme‑regulated
*AP0123062,384,7950TG24.3% 626.0 / 39.5 243N387T (AAC→ACC) cysNsulfate adenylyltransferase, subunit 1
*AP0123061,854,8510AC24.2% 633.8 / 64.5 253T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123061,393,3790AC24.0% 672.7 / 34.7 242T92P (ACC→CCC) nthDNA glycosylase and apyrimidinic (AP) lyase
*AP0123063,698,8280TG23.8% 738.3 / 41.4 273F68V (TTT→GTT) aphAacid phosphatase/phosphotransferase, class B, non‑specific
*AP012306406,6770AG23.6% 486.4 / 28.7 195K294E (AAA→GAA) hemHferrochelatase

Marginal new junction evidence (lowest skew 10 of 70 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 2073345 =401 (1.300)17 (0.080)
+43 bp
15/212 17.3 6.2% noncoding (1/76 nt) valU tRNA‑Val
?AP012306 = 2073665 328 (1.060)intergenic (+3/‑2) valY/lysV tRNA‑Val/tRNA‑Lys
* ? AP012306 643801 =316 (1.030)17 (0.080)
+43 bp
15/212 17.3 6.9% noncoding (1/76 nt) valZ tRNA‑Val
?AP012306 = 2073665 328 (1.060)intergenic (+3/‑2) valY/lysV tRNA‑Val/tRNA‑Lys
* ? AP012306 643498 =330 (1.070)17 (0.080)
+43 bp
15/212 17.3 6.8% noncoding (1/76 nt) valT tRNA‑Val
?AP012306 = 2073665 328 (1.060)intergenic (+3/‑2) valY/lysV tRNA‑Val/tRNA‑Lys
* ? AP012306 2073460 =270 (0.880)17 (0.070)
+38 bp
15/222 17.9 7.1% intergenic (+40/‑5) valU/valX tRNA‑Val/tRNA‑Val
?AP012306 = 2073665 328 (1.060)intergenic (+3/‑2) valY/lysV tRNA‑Val/tRNA‑Lys
* ? AP012306 2073345 =401 (1.300)13 (0.060)
+46 bp
13/206 17.9 5.0% noncoding (1/76 nt) valU tRNA‑Val
?AP012306 = 2073420 312 (1.010)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 643801 =316 (1.030)13 (0.060)
+46 bp
13/206 17.9 5.7% noncoding (1/76 nt) valZ tRNA‑Val
?AP012306 = 2073420 312 (1.010)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 643498 =330 (1.070)13 (0.060)
+46 bp
13/206 17.9 5.5% noncoding (1/76 nt) valT tRNA‑Val
?AP012306 = 2073420 312 (1.010)noncoding (76/76 nt) valU tRNA‑Val
* ? AP012306 643498 =330 (1.070)13 (0.060)
+45 bp
13/208 18.1 5.4% noncoding (1/76 nt) valT tRNA‑Val
?AP012306 = 643574 322 (1.050)intergenic (+1/‑2) valT/lysW tRNA‑Val/tRNA‑Lys
* ? AP012306 643801 =316 (1.030)13 (0.060)
+44 bp
13/210 18.2 6.3% noncoding (1/76 nt) valZ tRNA‑Val
?AP012306 = 643878 234 (0.760)intergenic (+2/‑2) valZ/lysY tRNA‑Val/tRNA‑Lys
* ? AP012306 643498 =330 (1.070)13 (0.060)
+44 bp
13/210 18.2 6.2% noncoding (1/76 nt) valT tRNA‑Val
?AP012306 = 643878 234 (0.760)intergenic (+2/‑2) valZ/lysY tRNA‑Val/tRNA‑Lys