breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 31 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AP0123061,091,9920TG38.8% 391.7 / 67.2 245intergenic (+38/‑145)narI/ychSnitrate reductase 1, gamma (cytochrome b(NR)) subunit/predicted protein
*AP0123061,944,2880AC32.7% 473.6 / 38.5 220G361G (GGT→GGGmenEo‑succinylbenzoate‑CoA ligase
*AP0123063,903,7910AG30.4% 477.0 / 85.5 241E189G (GAA→GGA) yjgJpredicted transcriptional regulator
*AP0123061,226,5570AG28.5% 321.3 / 80.0 165G55G (GGA→GGGydcUpredicted spermidine/putrescine transporter subunit
*AP012306644,4170GA28.4% 911.8 / inf 538intergenic (+32/‑401)lysQ/nadAtRNA‑Lys/quinolinate synthase, subunit A
*AP0123061,128,4120TG28.4% 710.6 / 57.9 299V29G (GTG→GGG) yciOconserved protein
*AP012306341,3770TG26.8% 208.3 / 12.4 113G60G (GGT→GGGribDfused diaminohydroxyphosphoribosylaminopyrimidin e deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
*AP0123061,854,8510AC26.3% 492.9 / 55.0 205T25P (ACC→CCC) yejHpredicted ATP‑dependet helicase
*AP0123063,938,8870CT25.7% 576.9 / 145.3 265S44F (TCT→TTT) bglJDNA‑binding transcriptional regulator
*AP0123063,527,3680AC25.3% 570.4 / 42.4 233L140F (TTA→TTCrhaSDNA‑binding transcriptional activator, L‑rhamnose‑binding
*AP012306935,0540GT25.0% 213.7 / 44.3 80intergenic (+24/‑1406)putP/ycdOproline:sodium symporter/conserved protein
*AP012306800,6750AC24.0% 546.0 / 25.8 221T192P (ACC→CCC) lolAchaperone for lipoproteins
*AP012306257,1370TG23.9% 442.2 / 11.0 201L496V (TTG→GTG) betTcholine transporter of high affinity
*AP0123063,282,0760TG23.4% 586.4 / 20.1 257intergenic (‑79/+27)ilvB/ivbLacetolactate synthase I, large subunit/ilvB operon leader peptide
*AP012306406,6770AG23.1% 431.6 / 23.7 173K294E (AAA→GAA) hemHferrochelatase
*AP012306202,4170TG22.9% 526.4 / 11.6 216V409G (GTA→GGA) tilStRNA(Ile)‑lysidine synthetase
*AP0123062,758,9090TG22.8% 604.9 / 15.8 232G33G (GGT→GGGagaDN‑acetylgalactosamine‑specific enzyme IID component of PTS
*AP0123062,560,2430TG22.8% 383.0 / 21.6 167N419T (AAC→ACC) pepPproline aminopeptidase P II
*AP0123063,182,8610AC22.7% 277.7 / 14.9 110L198F (TTA→TTCyiaNpredicted transporter
*AP0123063,590,3670AC22.1% 749.5 / 20.8 263T30P (ACC→CCC) fabRDNA‑binding transcriptional repressor

Marginal new junction evidence (lowest skew 10 of 229 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? AP012306 = 215364NA (NA)12 (0.040) 12/288 9.9 NA noncoding (745/2905 nt) rrlH 23S ribosomal RNA (rrlH)
?AP012306 = 215372 NA (NA)noncoding (753/2905 nt) rrlH 23S ribosomal RNA (rrlH)
* ? AP012306 = 997497317 (1.060)11 (0.040) 10/268 10.2 4.0% coding (41/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
?AP012306 997993 = 246 (0.920)coding (537/1305 nt) ndh respiratory NADH dehydrogenase 2/cupric reductase
* ? AP012306 = 3317647504 (1.690)10 (0.030) 9/290 10.8 2.0% intergenic (+242/‑1) trmE/tnaC GTPase/tryptophanase leader peptide
?AP012306 = 3317678 477 (1.640)coding (31/75 nt) tnaC tryptophanase leader peptide
* ? AP012306 = 1092512311 (1.040)13 (0.050) 7/258 11.0 4.7% intergenic (+100/+3) ychS/tpr predicted protein/predicted protamine‑like protein
?AP012306 1092985 = 257 (1.000)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? AP012306 228777 =311 (1.040)8 (0.030) 8/278 11.0 2.7% coding (275/771 nt) yafV predicted C‑N hydrolase family amidase, NAD(P)‑binding
?AP012306 228787 = 295 (1.060)coding (265/771 nt) yafV predicted C‑N hydrolase family amidase, NAD(P)‑binding
* ? AP012306 3671448 =427 (1.430)8 (0.030) 8/290 11.1 1.8% coding (20/1476 nt) xylE D‑xylose transporter
?AP012306 3671470 = 448 (1.540)intergenic (‑3/+369) xylE/malG D‑xylose transporter/maltose transporter subunit
* ? AP012306 = 1220777323 (1.080)7 (0.030) 7/270 11.2 2.3% coding (1435/1962 nt) ydcP predicted peptidase
?AP012306 = 1220763 299 (1.110)coding (1421/1962 nt) ydcP predicted peptidase
* ? AP012306 = 955136187 (0.630)9 (0.030) 8/292 11.2 4.7% intergenic (+1265/‑1406) mdoG/yceK glucan biosynthesis protein, periplasmic/predicted lipoprotein
?AP012306 = 955148 182 (0.620)intergenic (+1277/‑1394) mdoG/yceK glucan biosynthesis protein, periplasmic/predicted lipoprotein
* ? AP012306 259466 =387 (1.300)7 (0.030) 7/274 11.2 1.9% coding (907/1089 nt) yahA predicted DNA‑binding transcriptional regulator
?AP012306 259478 = 352 (1.280)coding (919/1089 nt) yahA predicted DNA‑binding transcriptional regulator
* ? AP012306 = 1452066377 (1.260)7 (0.020) 7/286 11.4 1.9% intergenic (+297/‑132) ydiK/ydiL predicted inner membrane protein/conserved protein
?AP012306 = 1452103 365 (1.270)intergenic (+334/‑95) ydiK/ydiL predicted inner membrane protein/conserved protein