breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 29 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*ACB1223,210,8810TG71.4% ‑1.1 / 18.6 28V204G (GTC→GGC) oprJ_2Outer membrane protein OprJ
*ACB122759,4330AC57.9% 3.5 / 15.5 21intergenic (+42/‑126)mmuM_1/CJ019_00729Homocysteine S‑methyltransferase/L‑methionine gamma‑lyase
*ACB1225,974,0620TG57.1% 1.0 / 12.2 28L684R (CTG→CGG) sasA_19Adaptive‑response sensory‑kinase SasA
*ACB1222,950,1660CG56.2% 4.7 / 16.6 16intergenic (+64/‑132)spuC_2/puuC_1Putrescine‑‑pyruvate aminotransferase/NADP/NAD‑dependent aldehyde dehydrogenase PuuC
*ACB1225,223,1590GC55.6% ‑4.2 / 16.2 27intergenic (‑133/+43)cntO_7/CJ019_04917Metal‑pseudopaline receptor CntO/hypothetical protein
*ACB1224,954,9120TG54.5% 6.1 / 11.2 11intergenic (‑129/+135)fliE/atoC_3Flagellar hook‑basal body complex protein FliE/Regulatory protein AtoC
*ACB122790,0070TG53.8% 1.5 / 15.4 26intergenic (+159/‑31)CJ019_00751/CJ019_00752hypothetical protein/hypothetical protein
*ACB1221,686,8560TG53.3% 4.8 / 11.5 15L90R (CTG→CGG) CJ019_01584hypothetical protein
*ACB1224,874,7090AC53.1% 4.6 / 18.1 32intergenic (+13/‑174)CJ019_04568/uacT_1hypothetical protein/Uric acid transporter UacT
*ACB1221,288,0410TG52.4% 8.2 / 17.6 21intergenic (+4/+66)slyB/nhaA_1Outer membrane lipoprotein SlyB/Na( )/H( ) antiporter NhaA
*ACB1223,134,5670TG52.2% 0.2 / 11.9 23L143V (TTG→GTG) CJ019_02952hypothetical protein
*ACB1224,245,1450AC51.8% 7.1 / 19.5 29L178R (CTG→CGG) CJ019_03982Crotonyl‑CoA hydratase
*ACB1224,135,6070AC50.0% 8.7 / 10.9 39Q32P (CAG→CCG) tgnC(Z)‑2‑((N‑methylformamido)methylene)‑5‑hydroxybu tyrolactone dehydrogenase
*ACB1222,938,2390AC46.7% 6.8 / 10.8 30K537N (AAA→AACpqiBIntermembrane transport protein PqiB
*ACB1221,524,7280TG43.6% 22.1 / 12.7 39P68P (CCA→CCCCJ019_01439hypothetical protein
*ACB1225,223,1640TC38.1% ‑1.1 / 11.3 27intergenic (‑138/+38)cntO_7/CJ019_04917Metal‑pseudopaline receptor CntO/hypothetical protein
*ACB1224,277,0430TG37.9% 21.5 / 11.9 29G278G (GGT→GGGhbd3‑hydroxybutyryl‑CoA dehydrogenase
*ACB1223,586,4040AG37.0% 26.9 / 10.3 32D128G (GAT→GGT) CJ019_03399hypothetical protein
*ACB1223,047,2170CG31.7% 86.5 / 11.1 45L122L (CTG→CTCCJ019_02862hypothetical protein
*ACB1221,084,2650TG31.4% 105.1 / 14.3 71R68R (CGT→CGGsodB_2Superoxide dismutase [Mn/Fe]

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? ACB122 = 293402945 (0.750)7 (0.120) 6/250 4.7 11.4% coding (204/912 nt) gltC_3 HTH‑type transcriptional regulator GltC
?ACB122 3005553 = 64 (1.080)coding (1557/1614 nt) bcsG Cellulose biosynthesis protein BcsG