breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 230 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273353,6010CA45.8% ‑3.3 / 16.6 24L267F (TTG→TTTcynRtranscriptional regulator CynR
*NZ_CP0092732,221,7540TC43.9% 57.9 / 18.1 57pseudogene (484/1436 nt)mdtQmultidrug resistance outer membrane protein MdtQ
*NZ_CP009273353,5860AT41.4% 22.2 / 12.0 32N272K (AAT→AAAcynRtranscriptional regulator CynR
*NZ_CP0092732,221,7180GC36.5% 71.8 / 14.3 52pseudogene (520/1436 nt)mdtQmultidrug resistance outer membrane protein MdtQ
*NZ_CP0092733,856,0370GA36.5% 6.3 / 27.8 55pseudogene (927/1617 nt)BW25113_RS19145PTS sugar transporter subunit IIB
*NZ_CP0092731,548,2570TG35.3% 120.5 / 18.3 90Y557S (TAC→TCC) maeAmalate dehydrogenase
*NZ_CP0092734,036,3900AG34.3% 107.4 / 19.0 67N206D (AAT→GAT) srkAstress response kinase SrkA
*NZ_CP0092733,856,0300TA34.0% 37.8 / 12.7 54pseudogene (934/1617 nt)BW25113_RS19145PTS sugar transporter subunit IIB
*NZ_CP0092732,221,7570GC33.9% 90.1 / 12.8 56pseudogene (481/1436 nt)mdtQmultidrug resistance outer membrane protein MdtQ
*NZ_CP0092733,856,0240CG33.3% 50.4 / 18.1 56pseudogene (940/1617 nt)BW25113_RS19145PTS sugar transporter subunit IIB
*NZ_CP0092733,856,0210TG32.1% 49.7 / 14.5 57pseudogene (943/1617 nt)BW25113_RS19145PTS sugar transporter subunit IIB
*NZ_CP0092732,221,7290AT32.1% 79.6 / 12.7 54pseudogene (509/1436 nt)mdtQmultidrug resistance outer membrane protein MdtQ
*NZ_CP0092734,594,1610CT32.0% 209.5 / 50.9 122S62F (TCT→TTT) bglJDNA‑binding transcriptional activator BglJ
*NZ_CP0092734,245,9970GC31.9% 138.7 / 21.2 71P133R (CCG→CGG) plsBglycerol‑3‑phosphate 1‑O‑acyltransferase PlsB
*NZ_CP0092734,277,6470TA31.5% 213.1 / 45.0 111intergenic (‑348/‑45)acs/nrfAacetate‑‑CoA ligase/ammonia‑forming nitrite reductase cytochrome c552 subunit
*NZ_CP009273883,4700TG31.2% 326.8 / 19.9 138intergenic (+55/+120)rcdA/ybjLDNA‑binding transcriptional regulator RcdA/aspartate:alanine antiporter
*NZ_CP0092731,980,4760AC30.7% 355.9 / 36.0 151Y24S (TAC→TCC) ftnBnon‑heme ferritin‑like protein
*NZ_CP0092732,221,7220GC30.2% 91.3 / 11.6 54pseudogene (516/1436 nt)mdtQmultidrug resistance outer membrane protein MdtQ
*NZ_CP0092733,856,0280AG29.6% 73.1 / 12.1 54pseudogene (936/1617 nt)BW25113_RS19145PTS sugar transporter subunit IIB
*NZ_CP009273748,5810TA29.0% 277.0 / 38.0 124intergenic (‑330/+60)ybgD/gltAfimbrial protein/citrate synthase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 1203246 =121 (0.760)27 (0.180) 27/464 9.6 18.1% coding (290/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 1205075 = 132 (0.890)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 69219782 (0.520)11 (0.100) 10/340 12.7 12.9% intergenic (‑1/+15) BW25113_RS03450/BW25113_RS03455 tRNA‑Met/tRNA‑Gln
?NZ_CP009273 692591 = 92 (0.840)intergenic (‑2/+378) BW25113_RS03470/asnB tRNA‑Met/asparagine synthase B
* ? NZ_CP009273 = 1203261113 (0.710)19 (0.130) 17/464 12.8 13.8% coding (305/630 nt) BW25113_RS05990 protein StfP
?NZ_CP009273 = 1205058 132 (0.890)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 294082672 (0.450)4 (0.040) 3/330 17.2 7.5% intergenic (+4/‑30) BW25113_RS14635/BW25113_RS14640 tRNA‑Met/tRNA‑Met
?NZ_CP009273 2941047 = 51 (0.480)intergenic (+5/+69) BW25113_RS14645/amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase AmiC
* ? NZ_CP009273 = 4532507211 (1.330)6 (0.040) 6/478 18.7 3.0% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 182 (1.190)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA