breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
JC 70,406 Δ6 bp 70.0% coding (20‑25/879 nt) araC → ara regulon transcriptional activator; autorepressor
RA 100,839 C→T 11.7% V25V (GTC→GTT murC → UDP‑N‑acetylmuramate:L‑alanine ligase
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 922,314:1 +C 5.7% intergenic (+21/+52) macB → / ← cspD macrolide ABC transporter peremase/ATPase/inhibitor of DNA replication, cold shock protein homolog
RA 924,050 T→G 28.2% F263V (TTT→GTT)  clpA → ATPase and specificity subunit of ClpA‑ClpP ATP‑dependent serine protease, chaperone activity
RA 1,036,090 G→A 5.4% A107A (GCG→GCA hyaD → hydrogenase 1 maturation protease
JC JC 1,198,576 IS2 (–) +5 bp 23.3% intergenic (‑339/‑115) ymfE ← / → lit e14 prophage; putative inner membrane protein/T4 phage exclusion protein; cell death peptidase, e14 prophage
RA 1,810,065 T→G 5.5% intergenic (+17/‑146) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
RA 1,810,066 C→A 5.0% intergenic (+18/‑145) yniC → / → ydjM 2‑deoxyglucose‑6‑P phosphatase/inner membrane protein regulated by LexA
MC JC 1,978,503 Δ776 bp 100% insB1insA insB1, insA
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,173,497 G→A 84.2% pseudogene (782/914 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,938,327 G→A 10.5% C287Y (TGC→TAC)  fucK → L‑fuculokinase
RA 2,939,592 C→A 80.9% S75R (AGC→AGA fucR → l‑fucose operon activator
RA 2,939,611 G→T 17.4% D82Y (GAT→TAT)  fucR → l‑fucose operon activator
RA 2,962,561 G→A 5.7% N68N (AAC→AAT) ‡ ppdC ← putative prepilin peptidase‑dependent protein
RA 2,962,562 T→C 5.7% N68S (AAC→AGC) ‡ ppdC ← putative prepilin peptidase‑dependent protein
RA 2,976,321 C→A 5.6% noncoding (65/82 nt) omrB ← sRNA antisense regulator downregulates OM proteins and curli; positively regulated by OmpR/EnvZ, Hfq‑dependent
RA 3,371,068 A→G 5.2% intergenic (‑32/+16) nanE ← / ← nanT putative N‑acetylmannosamine‑6‑P epimerase/sialic acid transporter
RA 3,541,500 C→T 29.7% S365F (TCC→TTC)  feoB → ferrous iron transporter protein B and GTP‑binding protein; membrane protein
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
JC 3,815,859 Δ82 bp 50.3% [rph][rph] [rph], [rph]
RA 4,148,049 C→G 5.3% G90A (GGC→GCC)  yijO ← AraC family putative transcriptional activator
RA 4,215,883 C→T 29.3% L136L (CTG→TTG)  aceB → malate synthase A
RA 4,296,190 A→G 19.3% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 20.6% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,268 T→C 28.8% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,286 C→T 15.5% intergenic (+492/+150) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423789–3424537 3424537 1–749 62 [61] [61] 62 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =62 (0.490)46 (0.390) 41/532 NT 46.1% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 49 (0.410)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780563 (0.500)55 (0.460) 51/532 NT 50.4% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 49 (0.410)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)166 (1.340) 136/556 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1411899 =127 (1.010)13 (0.110) 13/512 NT 10.5% coding (50/936 nt) ttcA tRNA s(2)C32 thioltransferase, iron sulfur cluster protein
?NC_000913 = 1434958 107 (0.940)pseudogene (51/51 nt) ttcC pseudogene, prophage Rac integration site ttcA duplication;Phage or Prophage Related
* ? NC_000913 2288919 =NA (NA)30 (0.240) 30/564 NT 16.1% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 4079792 156 (1.240)coding (42/219 nt) yiiF putative thymol sensitivity protein, CopG family putative transcriptional regulator
* ? NC_000913 3781698 =NA (NA)3 (0.020) 3/550 NT NA noncoding (1/29 nt) REP273 (repetitive extragenic palindromic) element; contains 1 REP sequences REP273 (repetitive extragenic palindromic) element; contains 1 REP sequences
?NC_000913 3781727 = NA (NA)intergenic (+39/+14) trmL/cysE tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent/serine acetyltransferase