breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 547,694 A→G E38E (GAA→GAG Y75_RS02655 → hypothetical protein
RA 547,835 +G coding (255/261 nt) Y75_RS02655 → hypothetical protein
RA 556,858 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase FolD
MC JC 654,214 Δ1,199 bp Y75_RS03225[dcuC] Y75_RS03225, [dcuC]
RA 909,411 G→A P102P (CCC→CCT ltaE ← low‑specificity L‑threonine aldolase
RA 987,574 G→T intergenic (‑170/+433) ompF ← / ← asnS porin OmpF/asparagine‑‑tRNA ligase
RA 1,093,686 T→C V130A (GTA→GCA)  Y75_RS05355 → GGDEF domain‑containing protein
RA 1,197,797 C→T H366H (CAC→CAT icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,809 C→T T370T (ACC→ACT icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,822 T→C L375M (TTA→CTG)  icd → NADP‑dependent isocitrate dehydrogenase
RA 1,197,824 A→G L375M (TTA→CTG icd → NADP‑dependent isocitrate dehydrogenase
MC JC 1,301,041 Δ1,336 bp Y75_RS06530Y75_RS06530 Y75_RS06530, Y75_RS06530
RA 1,337,160 G→A Q52Q (CAG→CAA Y75_RS06715 → hypothetical protein
RA 1,485,978 C→A R402S (CGT→AGT)  hrpA → ATP‑dependent RNA helicase HrpA
RA 1,519,222 G→T V40V (GTG→GTT Y75_RS07640 → orphan toxin OrtT
RA 1,592,479 C→A intergenic (‑223/+89) Y75_RS07945 ← / ← hipA helix‑turn‑helix transcriptional regulator/type II toxin‑antitoxin system serine/threonine protein kinase toxin HipA
RA 1,669,601 Δ1 bp coding (678/1221 nt) mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,704,037 Δ1 bp coding (91/1002 nt) add → adenosine deaminase
RA 1,979,957 G→T intergenic (‑46/+398) flhD ← / ← Y75_RS09980 flagellar transcriptional regulator FlhD/IS5‑like element IS5 family transposase
RA 2,005,401 C→T E115K (GAA→AAA)  fliC ← H48 family flagellin FliC
RA 2,156,723 C→A L191M (CTG→ATG)  mdtA → multidrug efflux RND transporter subunit MdtA
MC JC 2,176,983 Δ1,199 bp Y75_RS10980[gatA] Y75_RS10980, [gatA]
RA 2,245,455 A→G S283P (TCG→CCG)  Y75_RS11290 ← DUF418 family protein
MC JC 2,320,795 Δ1,336 bp Y75_RS11650[rcsC] Y75_RS11650, Y75_RS11650, [rcsC]
RA 2,447,095 C→T intergenic (‑44/‑115) fabB ← / → mnmC beta‑ketoacyl‑ACP synthase I/bifunctional tRNA (5‑methylaminomethyl‑2‑thiouridine)(34)‑methyltransferase MnmD/FAD‑dependent 5‑carboxymethylaminomethyl‑2‑thiouridine(34) oxidoreductase MnmC
RA 2,866,111 A→G pseudogene (97/993 nt) rpoS ← RNA polymerase sigma factor RpoS
RA 3,104,794 G→A G142D (GGC→GAC)  Y75_RS15435 → nucleoside permease
RA 3,212,340 A→C D213A (GAC→GCC)  rpoD → RNA polymerase sigma factor RpoD
MC JC 3,261,134 Δ1,199 bp Y75_RS16205[tdcD] Y75_RS16205, [tdcD]
RA 3,268,825 A→G T186A (ACA→GCA)  Y75_RS16240 → YhaC family protein
MC JC 3,742,936 Δ1,336 bp Y75_RS18560Y75_RS18560 Y75_RS18560, Y75_RS18560
MC JC 3,746,911 Δ1,199 bp Y75_RS18585[tnaB] Y75_RS18585, [tnaB]
MC JC 3,958,123 Δ34 bp coding (1156‑1189/1287 nt) Y75_RS19595 → C4‑dicarboxylate transporter DctC
JC 3,974,646 Δ78 bp coding (42‑119/825 nt) gadX → acid resistance transcriptional activator GadX
RA 4,114,118 C→A intergenic (‑171/‑149) igaA ← / → nudE intracellular growth attenuator protein IgaA/ADP compounds hydrolase NudE
MC JC 4,310,363 Δ1,199 bp Y75_RS21310[alsK] Y75_RS21310, [alsK]
RA 4,371,274 (A)5→3 pseudogene (179‑180/1304 nt) Y75_RS21600 ← anaerobic C4‑dicarboxylate transporter
RA 4,397,126 Δ1 bp intergenic (+11/‑26) Y75_RS21735 → / → Y75_RS21740 tRNA‑Gly/tRNA‑Gly
RA 4,397,133 C→T intergenic (+18/‑19) Y75_RS21735 → / → Y75_RS21740 tRNA‑Gly/tRNA‑Gly
RA 4,397,136 A→G intergenic (+21/‑16) Y75_RS21735 → / → Y75_RS21740 tRNA‑Gly/tRNA‑Gly
RA 4,405,094 G→A V43M (GTG→ATG)  hfq → RNA chaperone Hfq
RA 4,639,891 A→G S34P (TCC→CCC)  robA ← MDR efflux pump AcrAB transcriptional activator RobA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_007779 1197830 1213109 15280 119 [117] [117] 120 [icd]–[Y75_RS23470] [icd],Y75_RS05945,Y75_RS05950,lit,Y75_RS05960,Y75_RS05965,Y75_RS24820,Y75_RS23460,Y75_RS05970,Y75_RS05975,Y75_RS05980,ymfL,Y75_RS23465,Y75_RS05995,Y75_RS06000,Y75_RS06005,Y75_RS06010,Y75_RS06015,Y75_RS06020,Y75_RS06025,Y75_RS06030,Y75_RS06035,pinE,Y75_RS06045,[Y75_RS23470]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_007779 380484 =NA (NA)198 (1.030) 163/496 0.1 NA coding (28/444 nt) Y75_RS01845 transferase
?NC_007779 = 381407 NA (NA)coding (518/914 nt) Y75_RS01860 IS3‑like element IS2 family transposase
* ? NC_007779 = 68314314 (0.070)171 (0.890) 136/494 0.7 92.5% coding (673/1671 nt) Y75_RS03375 molecular chaperone HscC
?NC_007779 688274 = NA (NA)intergenic (‑105/+145) gltI/Y75_RS03405 glutamate/aspartate ABC transporter substrate‑binding protein GltI/IS5‑like element IS5 family transposase
* ? NC_007779 = 1398952NA (NA)215 (1.120) 149/496 0.4 92.3% intergenic (+146/‑127) Y75_RS07025/Y75_RS07030 IS5‑like element IS5 family transposase/DUF2534 family protein
?NC_007779 1574188 = 18 (0.090)coding (2729/2796 nt) Y75_RS07885 insulinase family protein
* ? NC_007779 = 157419122 (0.110)162 (0.840) 131/496 0.9 88.0% coding (2726/2796 nt) Y75_RS07885 insulinase family protein
?NC_007779 2176983 = NA (NA)intergenic (‑3/‑32) gatA/Y75_RS10980 PTS galactitol transporter subunit IIA/IS5‑like element IS5 family transposase
* ? NC_007779 1881541 =22 (0.110)188 (0.990) 151/492 0.3 89.6% coding (122/360 nt) yeaR DUF1971 domain‑containing protein
?NC_007779 = 2521060 NA (NA)intergenic (+171/+29) Y75_RS12570/Y75_RS12575 IS4‑like element IS421 family transposase/sensor domain‑containing phosphodiesterase
* ? NC_007779 = 188155117 (0.090)179 (0.940) 145/490 0.4 91.4% coding (112/360 nt) yeaR DUF1971 domain‑containing protein
?NC_007779 2519723 = NA (NA)intergenic (+33/‑54) nupC/Y75_RS12570 nucleoside permease NupC/IS4‑like element IS421 family transposase
* ? NC_007779 = 3186081NA (NA)179 (0.940) 140/494 0.6 96.8% coding (26/2466 nt) Y75_RS15850 fimbrial biogenesis outer membrane usher protein
?NC_007779 3512753 = 6 (0.030)coding (529/1026 nt) cytR DNA‑binding transcriptional regulator CytR
* ? NC_007779 = 35127593 (0.020)165 (0.860) 130/494 1.0 98.2% coding (535/1026 nt) cytR DNA‑binding transcriptional regulator CytR
?NC_007779 = 3744262 NA (NA)intergenic (‑45/+31) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
* ? NC_007779 = 3744263NA (NA)184 (0.960) 144/496 0.5 97.9% intergenic (‑46/+30) Y75_RS18560/yidZ IS3‑like element IS2 family transposase/HTH‑type transcriptional regulator YidZ
?NC_007779 3903274 = 4 (0.020)coding (37/825 nt) Y75_RS19350 protein bax
* ? NC_007779 = 37505900 (0.000)188 (1.000) 152/488 0.3 100% intergenic (‑104/‑33) tnaA/Y75_RS18600 tryptophanase/IS5‑like element IS5 family transposase
?NC_007779 3751790 = 0 (0.000)intergenic (+151/+116) Y75_RS18600/tnaC IS5‑like element IS5 family transposase/tryptophanase leader peptide
* ? NC_007779 = 39032794 (0.020)179 (0.940) 147/494 0.4 97.8% coding (42/825 nt) Y75_RS19350 protein bax
?NC_007779 = 4504190 NA (NA)intergenic (+10/+167) Y75_RS22280/Y75_RS22285 IS3‑like element IS2 family transposase/phosphoethanolamine transferase
* ? NC_007779 4310363 =NA (NA)209 (1.090) 134/496 0.8 90.1% intergenic (‑287/‑32) yjcS/Y75_RS21310 alkyl sulfatase YjcS/IS5‑like element IS5 family transposase
?NC_007779 4485284 = 23 (0.120)coding (875/1197 nt) Y75_RS22200 DUF898 domain‑containing protein
* ? NC_007779 = 4311556NA (NA)164 (0.860) 138/494 0.7 95.4% intergenic (+145/+1) Y75_RS21310/alsK IS5‑like element IS5 family transposase/allose kinase
?NC_007779 4540915 = 8 (0.040)intergenic (+204/+379) Y75_RS22495/Y75_RS22500 hypothetical protein/9‑O‑acetyl‑N‑acetylneuraminic acid deacetylase
* ? NC_007779 = 4311557NA (NA)218 (1.140) 157/496 0.2 90.8% pseudogene (921/921 nt) alsK allose kinase
?NC_007779 = 4485287 22 (0.110)coding (878/1197 nt) Y75_RS22200 DUF898 domain‑containing protein