breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 104,031 G→T intergenic (+70/‑161) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
RA 468,093 Δ1 bp coding (40/339 nt) glnK → nitrogen assimilation regulatory protein for GlnL, GlnE, and AmtB
RA 1,191,676 C→T H366H (CAC→CAT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,688 C→T T370T (ACC→ACT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,701 T→C L375M (TTA→CTG)  icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,703 A→G L375M (TTA→CTG icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 1,191,733 C→T N385N (AAC→AAT icd → e14 prophage; isocitrate dehydrogenase, specific for NADP+
RA 2,054,744 G→C T224R (ACG→AGG)  nac ← nitrogen assimilation regulon transcriptional regulator; autorepressor
RA 2,718,735 G→T P124Q (CCG→CAG)  kgtP ← alpha‑ketoglutarate transporter
RA 2,719,426 T→C intergenic (‑321/+2) kgtP ← / ← rrfG alpha‑ketoglutarate transporter/5S ribosomal RNA of rrnG operon
RA 3,304,872 G→T R58S (CGC→AGC)  rpsO ← 30S ribosomal subunit protein S15
RA 4,175,524 G→T V83F (GTC→TTC)  rpoC → RNA polymerase, beta prime subunit
RA 4,390,235 T→C I44T (ATC→ACC)  hfq → global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 345704 346935 1232 18 [17] [16] 18 prpC–[prpD] prpC, [prpD]
* * ÷ CP009273 748592 749952 1361 18 [16] [17] 18 gltA gltA
* * ÷ CP009273 1191736 1206911 15176 18 [17] [17] 18 [icd]–[icdC] 25 genes
[icd], ymfD, ymfE, lit, intE, xisE, ymfI, ymfJ, cohE, croE, ymfL, ymfM, oweE, aaaE, ymfR, beeE, jayE, ymfQ, stfP, tfaP, tfaE, stfE, pinE, mcrA, [icdC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =NA (NA)104 (2.240) 73/290 0.0 NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 = 1391495NA (NA)48 (1.030) 36/290 0.3 96.0% intergenic (+146/‑127) insH1/ynaJ IS5 transposase and trans‑activator/DUF2534 family putative inner membrane protein
?CP009273 = 1972751 2 (0.040)intergenic (‑297/‑483) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
* ? CP009273 1972748 =2 (0.040)71 (1.540) 44/288 0.1 97.3% intergenic (‑294/‑486) flhD/uspC flagellar class II regulon transcriptional activator, with FlhC/universal stress protein
?CP009273 = 2283592 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? CP009273 2269338 =1 (0.020)55 (1.190) 45/290 0.1 98.2% coding (587/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
?CP009273 = 3577555 NA (NA)intergenic (+15/‑1) insB1/yrhA IS1 transposase B/pseudogene, interrupted by IS1E
* ? CP009273 = 22693463 (0.060)47 (1.010) 40/290 0.2 94.0% coding (595/1026 nt) yejE microcin C transporter YejABEF, permease subunit; ABC family
?CP009273 3576788 = NA (NA)intergenic (+10/‑55) yrhA/insA pseudogene, interrupted by IS1E/IS1 repressor TnpA