breseq  version 0.32.0b  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 982,565 T→A intergenic (‑127/+476) ompF ← / ← asnS outer membrane porin 1a (Ia;b;F)/asparaginyl tRNA synthetase
RA 1,104,736 C→T intergenic (‑339/‑55) opgC ← / → opgG osmoregulated periplasmic glucan succinylation membrane protein/osmoregulated periplasmic glucan (OPG) biosynthesis periplasmic protein
RA 1,111,161 C→A R293L (CGT→CTT)  lpxL ← lauryl‑acyl carrier protein (ACP)‑dependent acyltransferase
RA 1,123,224 A→T intergenic (+39/‑71) yceM → / → murJ putative oxidoreductase/putative peptidoglycan lipid II flippase
RA 1,130,039 G→A intergenic (+26/‑146) flgF → / → flgG flagellar component of cell‑proximal portion of basal‑body rod/flagellar component of cell‑distal portion of basal‑body rod
RA 1,154,028 G→A G235D (GGC→GAC)  ptsG → fused glucose‑specific PTS enzymes: IIB component/IIC component
RA 2,083,827 G→C R52P (CGC→CCC)  hisG → ATP phosphoribosyltransferase
JC JC 2,400,257 IS5 (+) +4 bp intergenic (‑137/‑780) lrhA ← / → alaA transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2
RA 2,420,353 A→G intergenic (‑86/+135) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
RA 2,420,394 G→T intergenic (‑127/+94) hisJ ← / ← argT histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit
JC JC 3,178,254 IS5 (+) +4 bp coding (56‑59/291 nt) yqiC → BMFP family putative fusogenic protein
MC JC 3,360,110 Δ10,684 bp IS5‑mediated [yhcE]sspA [yhcE], yhcF, yhcG, yhcH, nanK, nanE, nanT, nanA, nanR, dcuD, sspB, sspA

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP009273 2084543 2085911 1369 45 [41] [43] 47 [hisG]–[hisC] [hisG], hisD, [hisC]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP009273 360104 =NA (NA)127 (1.460) 84/288 0.0 NA intergenic (‑38/+557) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
?CP009273 = 360286 NA (NA)intergenic (‑220/+375) lacZ/lacI pseudogene, truncated/DNA‑binding transcriptional repressor
* ? CP009273 1203246 =16 (0.180)56 (0.730) 45/256 1.0 78.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 1205075 = 17 (0.220)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? CP009273 = 120326116 (0.180)60 (0.780) 43/256 1.2 79.3% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?CP009273 = 1205058 17 (0.220)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related