mutation predictions | marginal predictions | summary statistics | genome diff | command line log
|
breseq version 0.32.0b
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
| Predicted mutations | ||||||
|---|---|---|---|---|---|---|
| evidence | position | mutation | freq | annotation | gene | description |
| RA | 41,501 | G→T | 25.1% | P144H (CCT→CAT) | caiT ← | putative transporter |
| RA | 367,764 | A→T | 19.6% | H65L (CAT→CTT) | mhpD → | 2‑keto‑4‑pentenoate hydratase |
| RA | 628,963 | C→G | 40.8% | intergenic (‑30/‑79) | ybdH ← / → ybdL | putative oxidoreductase/methionine aminotransferase, PLP‑dependent |
| RA | 684,444 | G→C | 46.1% | T9R (ACA→AGA) | insH1 ← | IS5 transposase and trans‑activator |
| RA | 1,062,550 | G→A | 16.7% | intergenic (‑3/+18) | yccJ ← / ← wrbA | uncharacterized protein/NAD(P)H:quinone oxidoreductase |
| JC JC | 2,400,257 | IS5 (+) +4 bp | 51.9% | intergenic (‑137/‑780) | lrhA ← / → alaA | transcriptional repressor of flagellar, motility and chemotaxis genes/valine‑pyruvate aminotransferase 2 |
| RA | 2,419,720 | T→C | 68.1% | D183G (GAT→GGT) | hisJ ← | histidine/lysine/arginine/ornithine transporter subunit |
| RA | 2,419,933 | G→T | 67.2% | A112D (GCC→GAC) | hisJ ← | histidine/lysine/arginine/ornithine transporter subunit |
| RA | 2,420,353 | A→G | 57.8% | intergenic (‑86/+135) | hisJ ← / ← argT | histidine/lysine/arginine/ornithine transporter subunit/lysine/arginine/ornithine transporter subunit |
| RA | 2,420,831 | T→C | 9.4% | Y147C (TAC→TGC) | argT ← | lysine/arginine/ornithine transporter subunit |
| RA | 2,434,063 | T→C | 12.8% | Q341R (CAG→CGG) | fabB ← | 3‑oxoacyl‑[acyl‑carrier‑protein] synthase I |
| RA | 2,493,637 | C→T | 10.5% | T496T (ACC→ACT) | ypdA → | sensor kinase regulating yhjX; pyruvate‑responsive YpdAB two‑component system |
| RA | 2,836,062 | T→A | 11.0% | Q114L (CAG→CTG) | hycI ← | protease involved in processing C‑terminal end of HycE |
| JC JC | 3,178,254 | IS5 (+) +4 bp | 46.7% | coding (56‑59/291 nt) | yqiC → | BMFP family putative fusogenic protein |
| RA | 3,219,839 | C→G | 53.9% | R83G (CGT→GGT) | ygjI → | putative transporter |
| RA | 3,319,555 | A→G | 18.6% | I247T (ATC→ACC) | ftsH ← | protease, ATP‑dependent zinc‑metallo |
| RA | 3,378,108:1 | +C | 45.5% | coding (47/471 nt) | argR → | l‑arginine‑responsive arginine metabolism regulon transcriptional regulator |
| RA | 3,664,009 | C→T | 16.1% | A353V (GCC→GTC) | treF → | cytoplasmic trehalase |
| RA | 3,849,032 | A→T | 19.6% | L100H (CTC→CAC) | yidG ← | inner membrane protein |
| RA | 4,048,586 | A→T | 100% | V18E (GTG→GAG) | glnG ← | fused DNA‑binding response regulator in two‑component regulatory system with GlnL: response regulator/sigma54 interaction protein |
| RA | 4,390,131:1 | +T | 39.1% | coding (27/309 nt) | hfq → | global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication |
| RA | 4,546,084 | G→T | 41.8% | H18Q (CAC→CAA) | yjiC ← | uncharacterized protein |
| Unassigned new junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | CP009273 | 360104 = | NA (NA) | 47 (2.170) | 39/272 | NT | NA | intergenic (‑38/+557) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor |
| ? | CP009273 | = 360286 | NA (NA) | intergenic (‑220/+375) | lacZ/lacI | pseudogene, truncated/DNA‑binding transcriptional repressor | |||||
| * | ? | CP009273 | 1973060 = | 9 (0.420) | 14 (0.650) | 14/272 | NT | 60.9% | intergenic (‑606/‑174) | flhD/uspC | flagellar class II regulon transcriptional activator, with FlhC/universal stress protein |
| ? | CP009273 | = 3577555 | NA (NA) | intergenic (+15/‑1) | insB1/yrhA | IS1 transposase B/pseudogene, interrupted by IS1E | |||||
| * | ? | CP009273 | = 1973068 | 9 (0.420) | 9 (0.420) | 8/272 | NT | 50.0% | intergenic (‑614/‑166) | flhD/uspC | flagellar class II regulon transcriptional activator, with FlhC/universal stress protein |
| ? | CP009273 | 3576788 = | NA (NA) | intergenic (+10/‑55) | yrhA/insA | pseudogene, interrupted by IS1E/IS1 repressor TnpA | |||||