breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 262,894 G→A 6.9% noncoding (24/76 nt) thrW → tRNA‑Thr
RA 354,812 C→T 100% intergenic (+220/‑110) prpE → / → codB propionate‑‑CoA ligase/cytosine transporter
RA 421,608 T→G 12.4% V208G (GTG→GGG)  proY → proline‑specific permease
RA 433,649 T→G 28.5% G65G (GGT→GGG ribD → fused diaminohydroxyphosphoribosylaminopyrimidine deaminase and 5‑amino‑6‑(5‑phosphoribosylamino) uracil reductase
RA 509,371 A→C 14.8% V670G (GTG→GGG)  copA ← copper transporter
RA 1,089,222 G→A 100% D212D (GAC→GAT pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
JC JC 1,293,008 IS1 (+) +9 bp 92.7% intergenic (‑86/‑511) hns ← / → tdk global DNA‑binding transcriptional dual regulator H‑NS/thymidine kinase/deoxyuridine kinase
RA 1,466,210 G→A 5.6% pseudogene (819/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
JC JC 1,879,829 Δ1 bp :: IS186 (+) +6 bp :: Δ1 bp 100% coding (115‑120/360 nt) yeaR ← DUF1971 family protein, nitrate‑inducible
JC JC 1,979,486 IS5 (+) +4 bp 100% intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
JC JC 1,995,341 IS5 (–) +4 bp 13.4% coding (16‑19/657 nt) uvrY ← response regulator in two‑component regulatory system with BarA
JC JC 1,995,398 IS5 (–) +4 bp 32.9% intergenic (‑39/‑417) uvrY ← / → yecF response regulator in two‑component regulatory system with BarA/DUF2594 family protein
RA 2,149,907 A→C 22.2% V360G (GTG→GGG)  yegI ← protein kinase‑related putative non‑specific DNA‑binding protein
RA 2,151,689 G→T 50.3% intergenic (+43/+22) yegJ → / ← yegK uncharacterized protein/ser/thr phosphatase‑related protein
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,304,523 G→C 5.5% intergenic (+130/+585) eco → / ← mqo ecotin, a serine protease inhibitor/malate dehydrogenase, FAD/NAD(P)‑binding domain
RA 2,818,145 T→C 12.0% intergenic (‑10/+53) argZ ← / ← argY tRNA‑Arg/tRNA‑Arg
RA 2,818,151 T→C 7.2% intergenic (‑16/+47) argZ ← / ← argY tRNA‑Arg/tRNA‑Arg
RA 2,828,510 G→T 10.7% M258I (ATG→ATT srlD → sorbitol‑6‑phosphate dehydrogenase
JC JC 2,876,678 IS5 (+) +4 bp 17.9% coding (98‑101/1038 nt) iap → aminopeptidase in alkaline phosphatase isozyme conversion
JC JC 2,916,566 IS1 (–) +9 bp 13.0% coding (1510‑1518/2757 nt) barA → hybrid sensory histidine kinase, in two‑component regulatory system with UvrY
RA 2,986,652 T→G 88.9% V269G (GTC→GGC)  yqeG → putative transporter
RA 3,560,455:1 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,782,913 A→C 22.0% V250G (GTG→GGG)  gpsA ← glycerol‑3‑phosphate dehydrogenase (NAD+)
RA 3,798,652 Δ1 bp 100% coding (661/1074 nt) waaU ← lipopolysaccharide core biosynthesis
MC JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,862,661 T→G 21.7% pseudogene (943/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
RA 3,862,662 T→G 25.6% pseudogene (942/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
RA 3,862,674:1 +A 6.9% pseudogene (930/1617 nt) glvC ← pseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
RA 4,183,597 G→T 100% D785Y (GAC→TAC)  rpoB → RNA polymerase, beta subunit
RA 4,296,060 C→T 31.4% intergenic (+266/+376) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380:1 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,325,948 A→G 5.9% intergenic (‑207/+451) yjdN ← / ← yjdM metalloprotein superfamily protein/zinc‑ribbon family protein
RA 4,571,096 A→G 26.4% L160P (CTC→CCC)  yjiR ← putative DNA‑binding transcriptional regulator/putative aminotransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423827–3424233 3424468–3424238 6–642 29 [27] [26] 29 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =26 (0.250)60 (0.640) 46/364 NT 71.5% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 24 (0.260)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780527 (0.260)50 (0.530) 39/364 NT 67.2% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 24 (0.260)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 124259477 (0.760)8 (0.090) 8/360 NT 10.3% coding (487/915 nt) ldcA murein tetrapeptide carboxypeptidase; LD‑carboxypeptidase A
?NC_000913 = 1242576 69 (0.740)coding (505/915 nt) ldcA murein tetrapeptide carboxypeptidase; LD‑carboxypeptidase A
* ? NC_000913 = 12994980 (0.000)57 (0.570) 41/388 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19785020 (0.000)80 (0.820) 52/380 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 = 199540158 (0.570)10 (0.100) 10/396 NT 14.7% intergenic (‑42/‑417) uvrY/yecF response regulator in two‑component regulatory system with BarA/DUF2594 family protein
?NC_000913 = 2102943 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 2807474 =58 (0.570)4 (0.040) 4/378 NT 5.1% coding (343/993 nt) proX glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
?NC_000913 2807610 = 94 (0.970)coding (479/993 nt) proX glycine betaine/proline ABC transporter periplasmic binding protein; cold shock protein
* ? NC_000913 3289715 =119 (1.170)6 (0.060) 4/374 NT 5.2% coding (902/2517 nt) yraJ putative outer membrane protein
?NC_000913 3289835 = 105 (1.090)coding (1022/2517 nt) yraJ putative outer membrane protein
* ? NC_000913 3621989 =NA (NA)5 (0.050) 3/380 NT 100% coding (2798/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 3622012 = 0 (0.000)coding (2821/4236 nt) rhsB Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 = 3670238122 (1.200)10 (0.100) 8/384 NT 7.6% coding (647/1650 nt) treF cytoplasmic trehalase
?NC_000913 = 3670407 124 (1.250)coding (816/1650 nt) treF cytoplasmic trehalase