breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 1,685,527 A→G G354G (GGT→GGC fumC ← fumarate hydratase (fumarase C),aerobic Class II
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,145,313 C→A S683Y (TCC→TAC)  yegE → putative diguanylate cyclase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,318,095 C→G L594F (TTG→TTC rcsC ← hybrid sensory kinase in two‑component regulatory system with RcsB and YojN
JC JC 3,173,112 IS5 (+) +4 bp intergenic (+1/‑27) mdaB → / → ygiN NADPH quinone reductase/quinol monooxygenase
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,993,145 C→A R665S (CGT→AGT)  cyaA → adenylate cyclase
RA 4,117,047 G→T S59Y (TCC→TAC)  glpK ← glycerol kinase
RA 4,129,994 Δ1 bp coding (160/2433 nt) metL → Bifunctional aspartokinase/homoserine dehydrogenase 2
RA 4,183,165 G→A E641K (GAA→AAA)  rpoB → RNA polymerase, beta subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)80 (0.820) 55/512 0.5 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3721045 =140 (1.420)161 (1.640) 96/518 0.0 54.4% noncoding (413/1443 nt) IS150 repeat region
?NC_000913 = 3839774 131 (1.330)coding (220/819 nt) nlpA cytoplasmic membrane lipoprotein‑28