breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp [crl] [crl]
RA 263,172 A→C pseudogene (41/63 nt) ykfN ← pseudogene, YdiA family
JC JC 572,447 IS2 (+) +5 bp coding (80‑84/102 nt) ylcH → uncharacterized protein, DLP12 prophage
RA 1,756,553 A→T K286* (AAA→TAA)  pykF → pyruvate kinase I
RA 1,914,888 T→G Q216P (CAG→CCG)  proQ ← RNA chaperone, putative ProP translation regulator
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
JC JC 1,979,486 IS5 (+) +4 bp intergenic (‑271/‑264) insA ← / → uspC IS1 repressor TnpA/universal stress protein
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 3,248,023 G→A W84* (TGG→TAG)  yqjA → general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter
RA 3,338,928 C→A L107F (TTG→TTT mlaE ← ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,684,604 G→A Q339* (CAG→TAG)  yhjK ← cyclic‑di‑GMP phosphodiesterase
MC JC 3,815,859 Δ82 bp [rph][rph] [rph], [rph]
RA 4,186,293 C→T A315V (GCG→GTG)  rpoC → RNA polymerase, beta prime subunit
RA 4,189,448 C→T Q1367* (CAG→TAG)  rpoC → RNA polymerase, beta prime subunit
RA 4,196,101 G→T intergenic (‑2/+231) thiC ← / ← rsd phosphomethylpyrimidine synthase/stationary phase protein, binds sigma 70 RNA polymerase subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,400,313 A→C D9A (GAT→GCT)  hfq → global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423686–3424608 3424608 1–923 70 [69] [69] 71 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =19 (0.160)73 (0.720) 57/166 0.9 83.0% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 14 (0.140)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780519 (0.160)71 (0.700) 56/166 1.0 82.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 14 (0.140)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)131 (1.130) 89/190 0.1 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 4359852 =114 (0.940)106 (0.880) 74/198 0.6 49.4% coding (180/1335 nt) cadB putative lysine/cadaverine transporter
?NC_000913 = 4375338 103 (0.850)coding (320/1029 nt) epmB EF‑P‑Lys34 lysylation protein; weak lysine 2,3‑aminomutase