breseq version 0.29.0 revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | [crl] | [crl] | |
RA | 263,172 | A→C | pseudogene (41/63 nt) | ykfN ← | pseudogene, YdiA family |
JC JC | 572,447 | IS2 (+) +5 bp | coding (80‑84/102 nt) | ylcH → | uncharacterized protein, DLP12 prophage |
RA | 1,756,553 | A→T | K286* (AAA→TAA) | pykF → | pyruvate kinase I |
RA | 1,914,888 | T→G | Q216P (CAG→CCG) | proQ ← | RNA chaperone, putative ProP translation regulator |
MC JC | 1,978,503 | Δ776 bp | insB1–insA | insB1, insA | |
JC JC | 1,979,486 | IS5 (+) +4 bp | intergenic (‑271/‑264) | insA ← / → uspC | IS1 repressor TnpA/universal stress protein |
RA | 2,173,363 | Δ2 bp | intergenic (‑1/+1) | gatC ← / ← gatC | pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC |
RA | 3,248,023 | G→A | W84* (TGG→TAG) | yqjA → | general envelope maintenance protein; DedA family inner membrane protein; putative multidrug efflux transporter |
RA | 3,338,928 | C→A | L107F (TTG→TTT) | mlaE ← | ABC transporter maintaining OM lipid asymmetry, inner membrane permease protein |
RA | 3,560,455 | +G | intergenic (‑2/+1) | glpR ← / ← glpR | pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon |
RA | 3,684,604 | G→A | Q339* (CAG→TAG) | yhjK ← | cyclic‑di‑GMP phosphodiesterase |
MC JC | 3,815,859 | Δ82 bp | [rph]–[rph] | [rph], [rph] | |
RA | 4,186,293 | C→T | A315V (GCG→GTG) | rpoC → | RNA polymerase, beta prime subunit |
RA | 4,189,448 | C→T | Q1367* (CAG→TAG) | rpoC → | RNA polymerase, beta prime subunit |
RA | 4,196,101 | G→T | intergenic (‑2/+231) | thiC ← / ← rsd | phosphomethylpyrimidine synthase/stationary phase protein, binds sigma 70 RNA polymerase subunit |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein |
RA | 4,400,313 | A→C | D9A (GAT→GCT) | hfq → | global sRNA chaperone; HF‑I, host factor for RNA phage Q beta replication |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 3423686–3424608 | 3424608 | 1–923 | 70 [69] | [69] 71 | [rrfD]–[rrlD] | [rrfD], [rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 1207790 = | 19 (0.160) | 73 (0.720) | 57/166 | 0.9 | 83.0% | coding (290/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | 1209619 = | 14 (0.140) | pseudogene (1/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1207805 | 19 (0.160) | 71 (0.700) | 56/166 | 1.0 | 82.6% | coding (305/630 nt) | stfP | e14 prophage; uncharacterized protein |
? | NC_000913 | = 1209602 | 14 (0.140) | pseudogene (18/501 nt) | stfE | pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 131 (1.130) | 89/190 | 0.1 | 100% | intergenic (+253/‑1684) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+1453/‑484) | ychE/oppA | UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | 4359852 = | 114 (0.940) | 106 (0.880) | 74/198 | 0.6 | 49.4% | coding (180/1335 nt) | cadB | putative lysine/cadaverine transporter |
? | NC_000913 | = 4375338 | 103 (0.850) | coding (320/1029 nt) | epmB | EF‑P‑Lys34 lysylation protein; weak lysine 2,3‑aminomutase |