breseq  version 0.28.1  revision 52323b585d6f
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 41 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*DH12,764,6950AT78.0% 798.2 / 204.4 366T149T (ACA→ACTmdtGmultidrug resistance protein MdtG
*DH12,187,5660GT76.8% 722.2 / 271.7 359P354Q (CCG→CAG) ydgAhypothetical protein
*DH11,456,6260AC73.7% 877.9 / inf 423Q47P (CAG→CCG) yfcFglutathione S‑transferase
*DH11,509,4850GT73.4% 697.7 / inf 414T139T (ACC→ACAarnCundecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase
*DH1810,3340GA72.7% 927.0 / inf 541W88* (TGG→TAG) gapAD‑erythrose‑4‑phosphate dehydrogenase
*DH1477,2650TG68.0% 925.9 / inf 537N195H (AAC→CAC) accCacetyl‑CoA carboxylase biotin carboxylase subunit
*DH1936,1690T.35.8% 220.9 / 126.6 97intergenic (‑13/+21)ECDH1_RS04615/ECDH1_RS04620tRNA‑Met/tRNA‑Met
*DH12,169,5770TG33.0% 180.9 / 71.6 221R661R (CGA→CGCrsxCelectron transporter RsxC
*DH13,906,8320CA33.0% 148.7 / 16.0 97intergenic (+3/‑32)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH1936,2930T.32.4% 307.3 / 166.7 144intergenic (‑27/+7)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH12,326,2870GT31.5% 668.9 / 126.0 407intergenic (+207/+200)yddM/fdnItranscriptional regulator/formate dehydrogenase nitrate‑inducible cytochrome b556(Fdn) subunit
*DH13,906,8370CG28.9% 161.0 / 25.6 105intergenic (+8/‑27)ECDH1_RS19315/ECDH1_RS19320tRNA‑Leu/tRNA‑Leu
*DH12,169,5480GT28.6% 364.9 / 72.3 228P671Q (CCG→CAG) rsxCelectron transporter RsxC
*DH1936,2970AG26.9% 372.4 / 146.2 203intergenic (‑31/+3)ECDH1_RS04620/ECDH1_RS04625tRNA‑Met/tRNA‑Met
*DH1613,7700CA26.0% 257.6 / 24.6 210intergenic (+631/+392)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH12,169,7200CG25.9% 151.3 / 45.5 152E614Q (GAA→CAA) rsxCelectron transporter RsxC
*DH1613,5970GC25.8% 395.2 / 49.9 266intergenic (+458/+565)garK/yhaCglycerate 2‑kinase/pentapeptide repeats‑related protein
*DH11,065,6050AT25.6% 330.3 / 110.0 220intergenic (+40/‑23)ECDH1_RS05245/ECDH1_RS05250tRNA‑Arg/tRNA‑Arg
*DH13,448,5260AC25.5% 775.2 / 85.2 418intergenic (‑86/+70)malZ/proYalpha‑glycosidase/proline‑specific permease ProY
*DH13,184,5850CA25.4% 366.5 / 35.5 182intergenic (+8/‑30)ECDH1_RS15795/ECDH1_RS15800tRNA‑Gln/tRNA‑TERM

Marginal new junction evidence (lowest skew 10 of 43 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? DH1 = 1104656283 (0.640)70 (0.160)
+CCAGAC
49/272 10.5 19.4% intergenic (+3959/‑748) ECDH1_RS05445/ypjA hypothetical protein/ATPase
?DH1 1104657 = 306 (0.660)intergenic (+3960/‑747) ECDH1_RS05445/ypjA hypothetical protein/ATPase
* ? DH1 1355966 =162 (0.350)122 (0.270)
+CTTC
52/292 10.8 35.7% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 289 (0.620)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355966 =162 (0.350)96 (0.210)
+CTTC
50/292 11.1 21.9% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3100565 540 (1.160)noncoding (1/76 nt) ECDH1_RS15385 tRNA‑Lys
* ? DH1 1355966 =162 (0.350)188 (0.410)
+CTTC
50/292 11.1 54.4% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099542 NA (NA)noncoding (268/268 nt) repeat_region repeat region
* ? DH1 3183985 =466 (1.000)68 (0.160)
+GATTGTTTCTTC
33/276 14.0 19.0% intergenic (+379/‑1) asnB/ECDH1_RS15770 asparagine synthetase B/tRNA‑Met
?DH1 = 3184256 166 (0.360)intergenic (+2/‑33) ECDH1_RS15780/ECDH1_RS15785 tRNA‑Gln/tRNA‑Gln
* ? DH1 = 2590941301 (0.640)50 (0.120) 28/260 14.7 21.2% intergenic (+1/‑209) ECDH1_RS12805/ECDH1_RS12810 tRNA‑Tyr/tRNA‑Tyr
?DH1 2591414 = 110 (0.270)noncoding (199/555 nt) repeat_region repeat region
* ? DH1 = 1355505439 (0.940)52 (0.130) 28/260 14.7 15.1% noncoding (54/348 nt) repeat_region repeat region
?DH1 3099693 = 204 (0.500)noncoding (58/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 1355966 =162 (0.350)105 (0.230)
+CTC
29/294 15.7 32.2% noncoding (76/76 nt) ECDH1_RS06685 tRNA‑Val
?DH1 = 3099750 289 (0.620)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys
* ? DH1 = 1519281318 (0.680)36 (0.080)
+AACCTTCTAATT
26/276 15.8 11.0% coding (788/900 nt) yfaD transposase
?DH1 1519282 = 318 (0.680)coding (787/900 nt) yfaD transposase
* ? DH1 1355844 =113 (0.240)78 (0.170)
+C
29/298 15.8 28.1% intergenic (‑45/+2) ECDH1_RS06675/ECDH1_RS06680 tRNA‑Val/tRNA‑Val
?DH1 = 3099750 289 (0.620)noncoding (1/76 nt) ECDH1_RS15360 tRNA‑Lys