breseq  version 0.24rc7  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0009133,838,9150A→T13.3% 53.1 ‑64.5 0.0603Y223F (TAT→TTT) yicLEamA family inner membrane putative transporter
*NC_0009131,101,7340G→T13.4% 50.5 ‑58.5 0.0523Q132K (CAA→AAA) csgFcurli nucleation outer membrane protein
*NC_000913821,4440G→T12.6% 40.0 ‑48.9 0.0502G218W (GGG→TGG) ybhMBAX Inhibitor‑1 family inner membrane protein
*NC_0009133,838,9190G→T13.9% 39.1 ‑67.5 0.0598L224F (TTG→TTTyicLEamA family inner membrane putative transporter
*NC_0009133,274,9280A→T12.7% 38.6 ‑53.5 0.0506intergenic (‑21/‑354)garP/garDputative (D)‑galactarate transporter/D‑galactarate dehydrogenase
*NC_0009131,101,7390C→A10.9% 36.4 ‑46.3 ‑0.0516G130V (GGT→GTT) csgFcurli nucleation outer membrane protein
*NC_0009132,551,2650C→A10.6% 35.1 ‑47.3 0.0539intergenic (‑49/+12)yfeY/yfeZRpoE‑regulated lipoprotein/inner membrane protein
*NC_0009133,274,9430A→T11.1% 30.1 ‑44.1 0.0487intergenic (‑36/‑339)garP/garDputative (D)‑galactarate transporter/D‑galactarate dehydrogenase
*NC_000913322,6520T→A13.2% 29.9 ‑59.8 0.0598N105K (AAT→AAAykgFferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component
*NC_0009133,274,9110A→T12.1% 29.8 ‑50.7 0.0495intergenic (‑4/‑371)garP/garDputative (D)‑galactarate transporter/D‑galactarate dehydrogenase
*NC_0009133,499,6920A→T10.1% 26.6 ‑41.2 0.0508intergenic (+77/‑218)yhfL/frlAsmall lipoprotein/putative fructoselysine transporter
*NC_0009131,478,3860C→T10.0% 25.2 ‑47.3 0.0510S54L (TCA→TTA) ynbBputative CDP‑diglyceride synthase
*NC_0009131,566,3400T→A10.8% 22.9 ‑40.0 0.0490K267N (AAA→AATdosCdiguanylate cyclase, cold‑ and stationary phase‑induced oxygen‑dependent biofilm regulator
*NC_0009131,825,2400A→G10.4% 20.5 ‑41.2 0.0551I129T (ATC→ACC) spyperiplasmic ATP‑independent protein refolding chaperone, stress‑induced
*NC_0009132,615,8270T→A10.2% 19.5 ‑34.8 0.0518I19F (ATT→TTT) yfgOputative UPF0118 family inner membrane permease
*NC_000913903,8730C→G10.0% 19.3 ‑42.3 0.0530intergenic (‑139/+79)artP/ybjParginine ABC transporter ATPase/lipoprotein
*NC_0009133,413,1630G→T10.9% 18.0 ‑52.0 0.0595A101A (GCC→GCAacrSacrAB operon transcriptional repressor
*NC_0009131,825,2650C→T10.0% 14.1 ‑44.6 0.0560E121K (GAA→AAA) spyperiplasmic ATP‑independent protein refolding chaperone, stress‑induced
*NC_0009132,899,9000T→G10.2% 12.4 ‑43.8 0.0588N125T (AAC→ACC) ygcWputative SDR family oxidoreductase
*NC_0009134,568,6080C→A11.0% 10.9 ‑50.6 ‑0.2520intergenic (‑89/‑390)mdtM/yjiPmultidrug efflux system protein/pseudogene, transposase_31 family protein
*NC_000913279,3980A→C100.0% 0.9 1V95G (GTG→GGG) insB1IS1 transposase B
*NC_00091315,6010G→C100.0% 0.0 1G53R (GGG→CGG) insL1IS186 transposase
*NC_00091315,6070A→G100.0% ‑0.1 1M55V (ATG→GTG) insL1IS186 transposase

Marginal mixed read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_000913283,9400A→G39.6% 14.2 ‑2.1 0.0111E247G (GAG→GGG) yagFCP4‑6 prophage; dehydratase family protein
*NC_000913686,0770G→C21.5% 10.3 ‑1.9 ‑0.0121A198G (GCG→GGG) gltJglutamate/aspartate ABC transporter permease
*NC_000913884,1840A→G22.4% 18.9 ‑0.1 0.0334E171G (GAG→GGG) mdfAmultidrug efflux system protein
*NC_0009132,151,5890A→T10.7% 15.4 ‑1.8 0.0228L135F (TTA→TTTyegJuncharacterized protein
*NC_0009132,151,6080G→A17.5% 24.9 ‑2.7 0.0217E142K (GAA→AAA) yegJuncharacterized protein
*NC_0009132,151,6890G→T37.4% 80.3 ‑1.1 0.0212intergenic (+43/+22)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
*NC_0009132,151,6910C→T17.3% 23.1 ‑1.2 ‑0.0230intergenic (+45/+20)yegJ/yegKuncharacterized protein/ser/thr phosphatase‑related protein
*NC_0009132,228,2780G→C30.0% 53.5 ‑0.1 0.0238pseudogene (332/1287 nt)mdtQpseudogene; putative channel/filament proteins
*NC_0009133,206,4770T→A18.1% 39.0 ‑0.8 0.0356S5R (AGT→AGAttdAL‑tartrate dehydratase, alpha subunit
*NC_0009133,206,4800T→A24.1% 78.9 ‑0.8 0.0356N6K (AAT→AAAttdAL‑tartrate dehydratase, alpha subunit
*NC_0009133,206,5060C→A16.5% 30.9 ‑0.9 ‑0.0373T15K (ACA→AAA) ttdAL‑tartrate dehydratase, alpha subunit
*NC_0009133,745,3710T→G16.9% 10.0 ‑1.7 0.0320W190G (TGG→GGG) yiaN2,3‑diketo‑L‑gulonate TRAP transporter large permease protein
*NC_0009133,862,6730G→A12.2% 14.4 ‑0.5 0.0282pseudogene (931/1617 nt)glvCpseudogene, arbutin specific enzyme IIC component of PTS;enzyme; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system, arbutin‑like IIB component; PTS system, arbutin‑like IIC component
*NC_0009134,027,1200C→T13.5% 12.3 ‑0.1 0.0308I198I (ATC→ATTfreNAD(P)H‑flavin reductase
*NC_0009134,296,2680T→C14.3% 21.5 ‑1.0 ‑0.185intergenic (+474/+168)gltP/yjcOglutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
*NC_0009134,458,4380T→G14.0% 12.0 ‑0.3 0.0340G160G (GGT→GGGpmbAputative antibiotic peptide MccB17 maturation peptidase
*NC_0009134,571,0810A→C24.0% 10.8 ‑0.3 0.0231V165G (GTG→GGG) yjiRputative DNA‑binding transcriptional regulator/putative aminotransferase

Marginal new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4542682 =500 (1.210)29 (0.070) 25/308 9.8 6.0% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542996 = 440 (1.130)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 = 382590NA (NA)31 (0.080) 24/326 10.7 5.9% noncoding (1331/1331 nt) IS2 repeat region
?NC_000913 564107 = 491 (1.190)coding (374/633 nt) sfmZ response regulator family protein
* ? NC_000913 381260 =NA (NA)27 (0.070) 18/324 12.6 5.1% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 564111 502 (1.220)coding (370/633 nt) sfmZ response regulator family protein
* ? NC_000913 3652036 =NA (NA)100 (0.240) 17/326 13.0 16.3% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 3890078 513 (1.240)coding (1349/1416 nt) tnaA tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
* ? NC_000913 = 2290113NA (NA)17 (0.040) 16/326 13.4 3.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 3890075 = 510 (1.230)coding (1346/1416 nt) tnaA tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
* ? NC_000913 = 4542690541 (1.310)13 (0.030) 11/308 15.1 2.7% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 = 4542986 440 (1.130)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 1097039 =226 (0.550)15 (0.040) 10/324 16.1 6.8% intergenic (+210/+526) ycdU/serX putative inner membrane protein/tRNA‑Ser
?NC_000913 = 1097580 189 (0.460)noncoding (73/88 nt) serX tRNA‑Ser
* ? NC_000913 = 3815858424 (1.030)10 (0.030) 9/306 16.1 2.8% pseudogene (24/48 nt) rph ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
?NC_000913 3815941 = 290 (0.750)pseudogene (609/669 nt) rph ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
* ? NC_000913 1097029 =230 (0.560)20 (0.050) 8/324 17.3 8.9% intergenic (+200/+536) ycdU/serX putative inner membrane protein/tRNA‑Ser
?NC_000913 = 1097570 180 (0.440)noncoding (83/88 nt) serX tRNA‑Ser
* ? NC_000913 = 2067353NA (NA)16 (0.040) 8/326 17.3 3.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 3890075 = 510 (1.230)coding (1346/1416 nt) tnaA tryptophanase/L‑cysteine desulfhydrase, PLP‑dependent
* ? NC_000913 2139570 =291 (0.700)6 (0.020) 6/304 17.9 2.3% intergenic (+85/+189) yegH/asmA inner membrane protein/suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
?NC_000913 2139603 = 243 (0.630)intergenic (+118/+156) yegH/asmA inner membrane protein/suppressor of OmpF assembly mutants; putative outer membrane protein assembly factor; inner membrane‑anchored periplasmic protein
* ? NC_000913 2688404 =340 (0.820)6 (0.020) 6/308 18.0 1.8% coding (400/1335 nt) glrR response regulator regulating glmY sRNA in two‑component system with sensor protein GlrK
?NC_000913 2688433 = 325 (0.830)coding (371/1335 nt) glrR response regulator regulating glmY sRNA in two‑component system with sensor protein GlrK
* ? NC_000913 217127 =495 (1.200)4 (0.010) 4/308 19.5 0.9% coding (1649/1719 nt) proS prolyl‑tRNA synthetase
?NC_000913 217154 = 457 (1.170)coding (1622/1719 nt) proS prolyl‑tRNA synthetase
* ? NC_000913 = 52601048 (0.120)4 (0.010) 4/316 19.8 7.4% coding (2750/4281 nt) rhsD Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 1528476 54 (0.130)pseudogene (575/2037 nt) rhsE pseudogene, Rhs family
* ? NC_000913 = 52601048 (0.120)4 (0.010) 4/316 19.8 8.5% coding (2750/4281 nt) rhsD Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 526022 40 (0.100)coding (2762/4281 nt) rhsD Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
* ? NC_000913 = 52602740 (0.100)4 (0.010) 4/316 19.8 7.4% coding (2767/4281 nt) rhsD Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
?NC_000913 = 1528459 61 (0.150)pseudogene (558/2037 nt) rhsE pseudogene, Rhs family
* ? NC_000913 598879 =374 (0.910)4 (0.010) 4/310 19.6 1.1% coding (166/3144 nt) cusA copper/silver efflux system, membrane component
?NC_000913 598946 = 354 (0.900)coding (233/3144 nt) cusA copper/silver efflux system, membrane component
* ? NC_000913 = 972756386 (0.930)4 (0.010) 4/314 19.7 1.0% coding (646/780 nt) elyC envelope biogenesis factor; DUF218 superfamily protein
?NC_000913 = 972773 406 (1.020)coding (629/780 nt) elyC envelope biogenesis factor; DUF218 superfamily protein
* ? NC_000913 1106995 =523 (1.270)4 (0.010) 4/306 19.5 0.8% coding (641/1422 nt) clsC stationary phase cardiolipin synthase 3
?NC_000913 1107021 = 507 (1.310)coding (667/1422 nt) clsC stationary phase cardiolipin synthase 3
* ? NC_000913 = 152846461 (0.150)4 (0.010) 4/316 19.8 6.6% pseudogene (563/2037 nt) rhsE pseudogene, Rhs family
?NC_000913 = 1528476 54 (0.130)pseudogene (575/2037 nt) rhsE pseudogene, Rhs family
* ? NC_000913 = 1985111531 (1.290)4 (0.010) 4/304 19.4 0.8% intergenic (‑42/+28) araG/araF L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein
?NC_000913 = 1985112 494 (1.280)intergenic (‑43/+27) araG/araF L‑arabinose ABC transporter ATPase/L‑arabinose ABC transporter periplasmic binding protein
* ? NC_000913 = 2032335421 (1.020)4 (0.010) 4/308 19.5 1.0% intergenic (‑18/+49) dcm/yedJ DNA cytosine methyltransferase/putative HD superfamily phosphohydrolase
?NC_000913 = 2032336 401 (1.030)intergenic (‑19/+48) dcm/yedJ DNA cytosine methyltransferase/putative HD superfamily phosphohydrolase
* ? NC_000913 2541926 =373 (0.900)4 (0.010) 4/312 19.7 1.1% coding (587/834 nt) cysU sulfate/thiosulfate ABC transporter permease
?NC_000913 2541944 = 352 (0.890)coding (569/834 nt) cysU sulfate/thiosulfate ABC transporter permease
* ? NC_000913 = 2595154370 (0.900)5 (0.010) 4/306 19.5 1.4% coding (706/2016 nt) tmcA elongator methionine tRNA (ac4C34) acetyltransferase
?NC_000913 = 2595179 347 (0.900)coding (681/2016 nt) tmcA elongator methionine tRNA (ac4C34) acetyltransferase
* ? NC_000913 2706126 =351 (0.850)4 (0.010) 4/310 19.6 1.2% coding (999/1800 nt) lepA back‑translocating elongation factor EF4, GTPase
?NC_000913 2706190 = 315 (0.800)coding (935/1800 nt) lepA back‑translocating elongation factor EF4, GTPase
* ? NC_000913 = 2930800319 (0.770)4 (0.010) 4/320 19.9 1.1% coding (1225/1368 nt) sdaB L‑serine dehydratase 2
?NC_000913 = 2930813 375 (0.930)coding (1238/1368 nt) sdaB L‑serine dehydratase 2
* ? NC_000913 = 3481674372 (0.900)4 (0.010) 4/314 19.7 1.1% coding (386/1914 nt) yheS ABC‑F family protein predicted regulatory ATPase
?NC_000913 = 3481686 369 (0.930)coding (398/1914 nt) yheS ABC‑F family protein predicted regulatory ATPase
* ? NC_000913 = 3493765NA (NA)4 (0.010) 4/310 19.6 NA noncoding (8/26 nt) REP248 (repetitive extragenic palindromic) element; contains 1 REP sequences REP248 (repetitive extragenic palindromic) element; contains 1 REP sequences
?NC_000913 = 3493775 NA (NA)noncoding (18/26 nt) REP248 (repetitive extragenic palindromic) element; contains 1 REP sequences REP248 (repetitive extragenic palindromic) element; contains 1 REP sequences
* ? NC_000913 3739408 =378 (0.920)4 (0.010) 4/314 19.7 1.1% coding (1912/2031 nt) malS alpha‑amylase
?NC_000913 3739435 = 376 (0.950)coding (1939/2031 nt) malS alpha‑amylase
* ? NC_000913 = 3986614320 (0.770)4 (0.010) 4/304 19.4 1.3% intergenic (‑607/+72) aslA/hemY putative Ser‑type periplasmic non‑aryl sulfatase/putative protoheme IX synthesis protein
?NC_000913 = 3986622 304 (0.790)intergenic (‑615/+64) aslA/hemY putative Ser‑type periplasmic non‑aryl sulfatase/putative protoheme IX synthesis protein
* ? NC_000913 = 4081273271 (0.660)4 (0.010) 4/308 19.5 1.5% coding (588/636 nt) fdoI formate dehydrogenase‑O, cytochrome b556 subunit
?NC_000913 = 4081294 262 (0.670)coding (567/636 nt) fdoI formate dehydrogenase‑O, cytochrome b556 subunit
* ? NC_000913 = 4139487351 (0.850)4 (0.010) 4/310 19.6 1.2% coding (222/663 nt) fsaB fructose‑6‑phosphate aldolase 2
?NC_000913 = 4139509 317 (0.810)coding (200/663 nt) fsaB fructose‑6‑phosphate aldolase 2
* ? NC_000913 4417665 =378 (0.920)4 (0.010) 4/310 19.6 1.1% coding (714/750 nt) yjfP acyl CoA esterase
?NC_000913 4417698 = 357 (0.910)coding (747/750 nt) yjfP acyl CoA esterase
* ? NC_000913 = 201031367 (0.890)4 (0.010) 3/310 20.5 1.2% coding (61/1026 nt) lpxD UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase
?NC_000913 = 201041 328 (0.840)coding (71/1026 nt) lpxD UDP‑3‑O‑(3‑hydroxymyristoyl)‑glucosamine N‑acyltransferase
* ? NC_000913 3461599 =321 (0.780)4 (0.010) 3/310 20.5 1.4% coding (132/510 nt) gspH putative general secretory pathway component, cryptic
?NC_000913 3461642 = 276 (0.700)coding (175/510 nt) gspH putative general secretory pathway component, cryptic
* ? NC_000913 = 3843338456 (1.100)4 (0.010) 3/306 20.3 0.9% coding (452/1335 nt) adeQ adenine permease, high affinity; adenine:H+ symporter
?NC_000913 4606393 = 427 (1.100)noncoding (9/87 nt) leuQ tRNA‑Leu
* ? NC_000913 224059 =NA (NA)3 (0.010) 3/312 20.5 NA noncoding (289/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
?NC_000913 224082 = NA (NA)noncoding (312/1542 nt) rrsH 16S ribosomal RNA of rrnH operon
* ? NC_000913 517076 =509 (1.230)3 (0.010) 3/320 20.8 0.6% coding (494/780 nt) fetB iron export ABC transporter permease; peroxide resistance protein
?NC_000913 517096 = 525 (1.300)coding (514/780 nt) fetB iron export ABC transporter permease; peroxide resistance protein
* ? NC_000913 = 582723423 (1.020)3 (0.010) 3/316 20.7 0.6% pseudogene (82/653 nt) ybcY pseudogene, DLP12 prophage; methyltransferase homology;Phage or Prophage Related
?NC_000913 = 1772679 594 (1.480)coding (168/1266 nt) ydiN putative MFS transporter, membrane protein
* ? NC_000913 692158 =464 (1.120)3 (0.010) 3/310 20.5 1.2% coding (184/468 nt) ybeY ssRNA‑specific endoribonuclease; 16S rRNA 3' end maturation and quality control co‑endoribonuclease working with RNase R; rRNA transcription antitermination factor
?NC_000913 3943790 = 58 (0.150)noncoding (87/2904 nt) rrlC 23S ribosomal RNA of rrnC operon
* ? NC_000913 750242 =462 (1.120)3 (0.010) 3/322 20.9 0.7% coding (1928/2448 nt) ybgQ putative outer membrane protein
?NC_000913 = 763537 432 (1.060)coding (524/1167 nt) sucC succinyl‑CoA synthetase, beta subunit
* ? NC_000913 = 1373284431 (1.040)3 (0.010) 3/310 20.5 0.8% coding (63/882 nt) ycjO putative sugar ABC transporter permease
?NC_000913 = 1373292 378 (0.960)coding (71/882 nt) ycjO putative sugar ABC transporter permease
* ? NC_000913 = 1634701318 (0.770)3 (0.010) 3/314 20.6 0.8% coding (185/576 nt) tfaQ Qin prophage; putative tail fibre assembly protein
?NC_000913 = 2850429 475 (1.190)coding (7/462 nt) hycA regulator of the transcriptional regulator FhlA
* ? NC_000913 = 1744340337 (0.820)3 (0.010) 3/310 20.5 0.9% coding (884/1374 nt) mdtK multidrug efflux system transporter
?NC_000913 = 1744392 304 (0.770)coding (936/1374 nt) mdtK multidrug efflux system transporter
* ? NC_000913 = 1801956484 (1.170)3 (0.010) 3/314 20.6 0.8% coding (615/1929 nt) thrS threonyl‑tRNA synthetase
?NC_000913 3090917 = 295 (0.740)coding (571/708 nt) endA DNA‑specific endonuclease I
* ? NC_000913 = 1979270NA (NA)8 (0.020) 3/326 21.0 1.6% noncoding (1/768 nt) IS1 repeat region
?NC_000913 2468910 = 479 (1.160)coding (697/921 nt) gtrB CPS‑53 (KpLE1) prophage; bactoprenol glucosyl transferase
* ? NC_000913 = 2076499339 (0.820)3 (0.010) 3/306 20.3 0.9% coding (192/447 nt) yeeS CP4‑44 prophage; putative DNA repair protein
?NC_000913 = 2076505 316 (0.820)coding (198/447 nt) yeeS CP4‑44 prophage; putative DNA repair protein
* ? NC_000913 = 2226253355 (0.860)3 (0.010) 3/316 20.7 0.9% coding (453/579 nt) yohD DedA family inner membrane protein
?NC_000913 = 2242835 359 (0.900)coding (133/1158 nt) yeiB DUF418 family putative inner membrane protein
* ? NC_000913 3119783 =309 (0.750)3 (0.010) 3/312 20.5 1.0% coding (1497/1683 nt) glcA glycolate transporter
?NC_000913 3119805 = 307 (0.780)coding (1475/1683 nt) glcA glycolate transporter
* ? NC_000913 3186096 =NA (NA)10 (0.020) 3/326 21.0 2.0% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 3767092 = 484 (1.170)coding (756/843 nt) yibA putative immunity protein for polymorphic toxin RhsA; HEAT‑domain protein; lethality reduction protein
* ? NC_000913 4017176 =509 (1.230)3 (0.010) 3/312 20.5 0.6% coding (746/762 nt) udp uridine phosphorylase
?NC_000913 4017222 = 475 (1.200)intergenic (+30/‑111) udp/rmuC uridine phosphorylase/DNA recombination protein
* ? NC_000913 4434563 =386 (0.930)3 (0.010) 3/310 20.5 0.8% noncoding (68/111 nt) REP334 (repetitive extragenic palindromic) element; contains 3 REP sequences REP334 (repetitive extragenic palindromic) element; contains 3 REP sequences
?NC_000913 4434590 = 401 (1.020)noncoding (95/111 nt) REP334 (repetitive extragenic palindromic) element; contains 3 REP sequences REP334 (repetitive extragenic palindromic) element; contains 3 REP sequences