breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 225,805 C→A noncoding (47/2904 nt) rrlH → 23S ribosomal RNA
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 415,660 G→T L32M (CTG→ATG)  sbcC ← ATP dependent, structure specific DNA nuclease ‑ SbcC subunit
RA 561,422 G→T D576Y (GAT→TAT)  sfmD → putative fimbrial usher protein SfmD
RA 717,257 C→A A337S (GCA→TCA)  potE ← putrescine:H(+) symporter/putrescine:ornithine antiporter PotE
RA 846,090 C→A G125V (GGT→GTT)  glnQ ← L‑glutamine ABC transporter ATP binding subunit
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 974,255 C→T P240L (CCG→CTG)  cmoM → tRNA cmo(5)U34 methyltransferase
RA 1,089,912 C→A R793I (AGA→ATA)  pgaA ← partially deacetylated poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine outer membrane porin
MC JC 1,233,317 Δ10 bp coding (1392‑1401/1542 nt) nhaB ← Na(+):H(+) antiporter NhaB
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
RA 1,727,994 C→A A53E (GCG→GAG)  gloA → glyoxalase I
RA 1,817,420 G→T L361M (CTG→ATG)  chbF ← monoacetylchitobiose‑6‑phosphate hydrolase
RA 1,822,260 C→A intergenic (‑4/‑198) osmE ← / → nadE osmotically‑inducible lipoprotein OsmE/NAD synthetase, NH3‑dependent
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,011,090 G→T intergenic (+11/+133) yedL → / ← yedN putative acetyltransferase YedL/putative type III secreted effector, N‑terminal fragment
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,260,694 C→A P239P (CCG→CCT fruA ← fructose‑specific PTS multiphosphoryl transfer protein FruA
RA 2,441,238 G→T P123Q (CCG→CAG)  fabB ← beta‑ketoacyl‑[acyl carrier protein] synthase I
RA 3,000,624 C→A R94S (CGT→AGT)  xdhA → putative xanthine dehydrogenase molybdenum‑binding subunit XdhA
RA 3,141,233 G→T I18I (ATC→ATA hybD ← putative hydrogenase 2 maturation protease
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,538,065 C→A F227L (TTC→TTA yhgF → putative RNA‑binding protein YhgF
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,791,325 C→A L7L (CTG→CTT tdh ← threonine dehydrogenase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,927,437 C→A H95N (CAC→AAC)  asnA → asparagine synthetase A
RA 4,018,140 G→T A270S (GCC→TCC)  rmuC → putative recombination limiting protein RmuC
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,354,654 G→T R22S (CGC→AGC)  lysU ← lysine‑‑tRNA ligase/Ap4A synthetase/Ap3A synthetase
JC 4,527,042 +GCA coding (496/633 nt) sgcE ← KpLE2 phage‑like element; putative epimerase SgcE

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102972 3104072 1101 51 [47] [50] 52 mutY mutY
* * ÷ NC_000913 3423754–3424233 3424527–3424239 7–774 51 [49] [50] 51 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =80 (0.460)225 (1.460) 85/244 0.1 75.0% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 79 (0.510)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 120780580 (0.460)120 (0.780) 69/244 0.4 61.6% coding (305/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 = 1209602 79 (0.510)pseudogene (54/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)257 (1.510) 105/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =250 (1.430)265 (1.530) 101/274 0.1 67.8% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)254 (1.450) 96/276 0.1 96.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 9 (0.050)coding (3907/4134 nt) rhsA rhs element protein RhsA