breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 19,567 C→A Q285K (CAG→AAG)  nhaR → DNA‑binding transcriptional activator NhaR
RA 65,483 C→A G100C (GGC→TGC)  polB ← DNA polymerase II
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 628,164 T→C C157C (TGT→TGC entB → enterobactin synthase component B
RA 663,547 C→A S139S (TCG→TCT dacA ← D‑alanyl‑D‑alanine carboxypeptidase DacA
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 921,374 C→A P343Q (CCG→CAG)  macB → ABC‑type tripartite efflux pump ATP binding/membrane subunit
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,281,999 C→A I712I (ATC→ATA narG → nitrate reductase A subunit alpha
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,525,144 G→T A758A (GCG→GCT mrcA → peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,573,632 C→A intergenic (‑130/+143) glgB ← / ← asd 1,4‑alpha‑glucan branching enzyme/aspartate‑semialdehyde dehydrogenase
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,941,512 C→A intergenic (‑185/‑296) yieP ← / → rrsC putative transcriptional regulator YieP/16S ribosomal RNA
RA 4,045,713 C→A R297L (CGG→CTG)  yihG ← putative acyltransferase YihG
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196255 1211433 15179 36 [33] [31] 39 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102982 3104050 1069 36 [31] [34] 35 [mutY] [mutY]
* * ÷ NC_000913 3423770–3424234 3424531–3424239 6–762 37 [34] [32] 37 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 374921 =NA (NA)43 (0.510) 18/190 2.8 NA intergenic (+40/‑538) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377531 = NA (NA)intergenic (+220/+4) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 374963NA (NA)50 (0.600) 18/190 2.8 NA noncoding (33/34 nt) other REP31a
?NC_000913 = 377487 NA (NA)intergenic (+176/+48) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375203 =NA (NA)72 (0.750) 26/216 2.1 NA noncoding (17/36 nt) other REP31f
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 = 375219NA (NA)27 (0.280) 20/216 2.9 NA noncoding (1/36 nt) other REP31f
?NC_000913 = 377333 NA (NA)intergenic (+22/+202) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 = 12994980 (0.000)129 (1.090) 68/268 0.3 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3362982 =148 (1.210)150 (1.250) 71/272 0.2 40.1% coding (176/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 = 3363113 302 (2.510)coding (307/2382 nt) yhcD putative fimbrial usher protein YhcD
* ? NC_000913 3363001 =151 (1.240)168 (1.380) 79/274 0.1 68.6% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)143 (1.170) 80/276 0.1 98.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 3 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA