breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
RA | 19,567 | C→A | Q285K (CAG→AAG) | nhaR → | DNA‑binding transcriptional activator NhaR |
RA | 65,483 | C→A | G100C (GGC→TGC) | polB ← | DNA polymerase II |
MC JC | 257,908 | Δ776 bp | insB9–[crl] | insB9, insA9, [crl] | |
RA | 628,164 | T→C | C157C (TGT→TGC) | entB → | enterobactin synthase component B |
RA | 663,547 | C→A | S139S (TCG→TCT) | dacA ← | D‑alanyl‑D‑alanine carboxypeptidase DacA |
RA | 889,923 | A→G | intergenic (‑104/‑166) | ybjL ← / → ybjM | putative transport protein YbjL/putative inner membrane protein |
RA | 921,374 | C→A | P343Q (CCG→CAG) | macB → | ABC‑type tripartite efflux pump ATP binding/membrane subunit |
RA | 1,196,220 | C→T | H366H (CAC→CAT) | icd → | isocitrate dehydrogenase |
RA | 1,196,232 | C→T | T370T (ACC→ACT) | icd → | isocitrate dehydrogenase |
RA | 1,196,245 | T→C | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase |
RA | 1,196,247 | A→G | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase |
RA | 1,281,999 | C→A | I712I (ATC→ATA) | narG → | nitrate reductase A subunit alpha |
RA | 1,678,604 | G→T | A60S (GCA→TCA) | ydgH → | DUF1471 domain‑containing protein YdgH |
JC JC | 1,879,829 | Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp | coding (115‑120/360 nt) | yeaR ← | DUF1971 domain‑containing protein YeaR |
MC JC | 1,978,503 | Δ776 bp | insB‑5–insA‑5 | insB‑5, insA‑5 | |
RA | 2,173,363 | Δ2 bp | pseudogene (915‑916/1358 nt) | gatC ← | galactitol‑specific PTS enzyme IIC component |
RA | 3,354,487 | C→T | intergenic (‑437/‑238) | yhcC ← / → gltB | radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB |
RA | 3,525,144 | G→T | A758A (GCG→GCT) | mrcA → | peptidoglycan glycosyltransferase/peptidoglycan DD‑transpeptidase MrcA |
RA | 3,560,455 | +G | pseudogene (151/758 nt) | glpR ← | DNA‑binding transcriptional repressor GlpR |
RA | 3,573,632 | C→A | intergenic (‑130/+143) | glgB ← / ← asd | 1,4‑alpha‑glucan branching enzyme/aspartate‑semialdehyde dehydrogenase |
RA | 3,815,883 | +C | coding (667/687 nt) | rph ← | truncated RNase PH |
RA | 3,941,512 | C→A | intergenic (‑185/‑296) | yieP ← / → rrsC | putative transcriptional regulator YieP/16S ribosomal RNA |
RA | 4,045,713 | C→A | R297L (CGG→CTG) | yihG ← | putative acyltransferase YihG |
RA | 4,261,710 | C→A | S14R (AGC→AGA) | dusA → | tRNA‑dihydrouridine synthase A |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 1196255 | 1211433 | 15179 | 36 [33] | [31] 39 | [icd]–[icdC] | [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC] |
* | * | ÷ | NC_000913 | 3102982 | 3104050 | 1069 | 36 [31] | [34] 35 | [mutY] | [mutY] |
* | * | ÷ | NC_000913 | 3423770–3424234 | 3424531–3424239 | 6–762 | 37 [34] | [32] 37 | [rrfD]–[rrlD] | [rrfD],[rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 374921 = | NA (NA) | 43 (0.510) | 18/190 | 2.8 | NA | intergenic (+40/‑538) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377531 = | NA (NA) | intergenic (+220/+4) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | = 374963 | NA (NA) | 50 (0.600) | 18/190 | 2.8 | NA | noncoding (33/34 nt) | other | REP31a |
? | NC_000913 | = 377487 | NA (NA) | intergenic (+176/+48) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 375203 = | NA (NA) | 72 (0.750) | 26/216 | 2.1 | NA | noncoding (17/36 nt) | other | REP31f |
? | NC_000913 | 377351 = | NA (NA) | noncoding (16/37 nt) | other | REP32a | |||||
* | ? | NC_000913 | = 375219 | NA (NA) | 27 (0.280) | 20/216 | 2.9 | NA | noncoding (1/36 nt) | other | REP31f |
? | NC_000913 | = 377333 | NA (NA) | intergenic (+22/+202) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | = 1299498 | 0 (0.000) | 129 (1.090) | 68/268 | 0.3 | 100% | intergenic (+253/‑33) | ychE/insH21 | putative inner membrane protein/IS5 transposase and trans‑activator |
? | NC_000913 | 1300698 = | 0 (0.000) | intergenic (+151/‑484) | insH21/oppA | IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein | |||||
* | ? | NC_000913 | 3362982 = | 148 (1.210) | 150 (1.250) | 71/272 | 0.2 | 40.1% | coding (176/2382 nt) | yhcD | putative fimbrial usher protein YhcD |
? | NC_000913 | = 3363113 | 302 (2.510) | coding (307/2382 nt) | yhcD | putative fimbrial usher protein YhcD | |||||
* | ? | NC_000913 | 3363001 = | 151 (1.240) | 168 (1.380) | 79/274 | 0.1 | 68.6% | coding (195/2382 nt) | yhcD | putative fimbrial usher protein YhcD |
? | NC_000913 | 3766087 = | 4 (0.030) | coding (3905/4134 nt) | rhsA | rhs element protein RhsA | |||||
* | ? | NC_000913 | = 3653230 | NA (NA) | 143 (1.170) | 80/276 | 0.1 | 98.0% | noncoding (1/1195 nt) | IS5 | repeat region |
? | NC_000913 | = 3766089 | 3 (0.020) | coding (3907/4134 nt) | rhsA | rhs element protein RhsA |