breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 58,194 C→A intergenic (+15/‑280) djlA → / → yabP co‑chaperone protein DjlA/putative uncharacterized protein YabP
RA 60,788 G→T A826D (GCT→GAT)  rapA ← RNA polymerase‑binding ATPase and RNAP recycling factor
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
MC JC 338,477 Δ60 bp coding (153‑212/1419 nt) yahG → DUF1116 domain‑containing protein YahG
RA 674,868 C→A intergenic (‑85/‑150) leuS ← / → ybeL leucine‑‑tRNA ligase/DUF1451 domain‑containing protein YbeL
RA 830,388 C→A A86S (GCG→TCG)  cecR ← DNA‑binding transcriptional dual regulator CecR
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,645,663 C→A K70N (AAG→AAT relB ← Qin prophage; antitoxin/DNA‑binding transcriptional repressor RelB
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,105,039 G→A R16W (CGG→TGG)  wbbJ ← putative acyl transferase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,197,838 G→T intergenic (+540/‑2441) yehF → / → yehI putative protein YehF/DUF4132 domain‑containing protein YehI
RA 2,280,018 Δ1 bp coding (466/696 nt) rsuA ← 16S rRNA pseudouridine(516) synthase
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 4,140,124 C→A D700Y (GAC→TAC)  ptsA ← putative PTS multiphosphoryl transfer protein PtsA
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,344,543 G→T G392V (GGG→GTG)  melB → melibiose:H(+)/Na(+)/Li(+) symporter
RA 4,505,167 C→A G370G (GGC→GGA yjhB → putative sialic acid transporter

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196259 1211438 15180 28 [27] [27] 28 [icd]–[icdC] [icd],ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,oweE,ymfN,aaaE,ymfR,beeE,jayE,ymfQ,ycfK,tfaP,tfaE,stfE,pinE,mcrA,[icdC]
* * ÷ NC_000913 3102983 3104069 1087 28 [27] [27] 32 mutY mutY
* * ÷ NC_000913 3423770–3424234 3424535–3424239 6–766 28 [24] [26] 28 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 375023 =NA (NA)33 (0.490) 15/192 2.7 NA intergenic (+142/‑436) mhpE/mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
?NC_000913 377530 = NA (NA)intergenic (+219/+5) yaiL/frmB DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB
* ? NC_000913 375203 =NA (NA)54 (0.710) 24/216 1.8 NA noncoding (17/36 nt) other REP31f
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 = 12994980 (0.000)99 (1.050) 59/268 0.3 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =141 (1.450)172 (1.790) 79/274 0.0 70.6% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 3 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)132 (1.360) 75/276 0.1 95.7% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 6 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA