breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 375,168 G→A intergenic (+287/‑291) mhpE → / → mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
RA 375,176 A→G intergenic (+295/‑283) mhpE → / → mhpT 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter
RA 809,328 G→T intergenic (‑71/‑16) bioA ← / → bioB adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase/biotin synthase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
JC 1,198,460 (AATGAAAT)3→4 intergenic (‑223/‑235) ymfE ← / → lit e14 prophage; uncharacterized protein YmfE/e14 prophage; cell death peptidase Lit
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,748,665 G→T R235L (CGC→CTC)  ypjD → cytochrome c assembly family protein
RA 3,118,076 G→T A346E (GCG→GAG)  yghJ ← putative lipoprotein YghJ
RA 3,290,662 C→A L617M (CTG→ATG)  yraJ → putative fimbrial usher protein YraJ
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,466,935 G→C R19R (CGC→CGG bfd ← bacterioferritin‑associated ferredoxin
RA 3,495,322 G→T A438S (GCC→TCC)  nirB → nitrite reductase catalytic subunit NirB
RA 3,511,065 C→A V120F (GTT→TTT)  yhfY ← conserved protein YhfY
RA 3,535,990 G→T R199S (CGC→AGC)  ompR ← DNA‑binding transcriptional dual regulator OmpR
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,897,522 G→T R6L (CGG→CTG)  cbrB → putative inner membrane protein
RA 4,140,133 G→T L697M (CTG→ATG)  ptsA ← putative PTS multiphosphoryl transfer protein PtsA
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102972 3104086 1115 35 [32] [34] 36 mutY mutY
* * ÷ NC_000913 3423749–3424234 3424540–3424239 6–792 35 [34] [34] 37 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)97 (1.000) 63/268 0.2 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =136 (1.360)169 (1.700) 82/274 0.0 71.2% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 2 (0.020)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)100 (1.000) 70/276 0.1 98.0% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 2 (0.020)coding (3907/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 4542682 =72 (0.720)27 (0.290) 25/258 2.7 30.1% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 58 (0.620)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269072 (0.720)28 (0.300) 24/258 2.8 30.9% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 58 (0.620)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit