breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 530,180 G→T V349V (GTC→GTA selU ← tRNA 2‑selenouridine synthase
RA 731,083 C→A P501T (CCT→ACT)  rhsC → rhs element protein RhsC
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 1,332,361 G→T M438I (ATG→ATT topA → DNA topoisomerase 1
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,337,712 G→T A570E (GCA→GAA)  gyrA ← DNA gyrase subunit A
RA 2,847,744 G→A Q500* (CAA→TAA)  hycC ← formate hydrogenlyase subunit HycC
RA 2,865,734 C→A L212M (CTG→ATG)  ygbN → putative transporter YgbN
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,895,532 C→A G360C (GGT→TGT)  adeP ← adenine:H(+) symporter
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,416,608 G→T R55R (CGA→AGA)  bsmA ← DUF1471 domain‑containing putative lipoprotein BsmA
RA 4,598,064 C→A G460W (GGG→TGG)  opgB ← phosphoglycerol transferase I

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102955 3104069 1115 37 [36] [36] 40 mutY mutY
* * ÷ NC_000913 3423769–3424234 3424543–3424239 6–775 37 [36] [36] 37 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)96 (0.820) 56/268 0.6 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =171 (1.420)196 (1.640) 90/274 0.0 69.3% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)141 (1.170) 68/276 0.3 94.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 8 (0.070)coding (3907/4134 nt) rhsA rhs element protein RhsA