breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 100,982 C→A P73Q (CCG→CAG)  murC → UDP‑N‑acetylmuramate‑‑L‑alanine ligase
RA 233,152 C→A P268P (CCG→CCT mltD ← membrane‑bound lytic murein transglycosylase D
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 498,499 C→A R149S (CGC→AGC)  hemH → ferrochelatase
RA 889,923 A→G intergenic (‑104/‑166) ybjL ← / → ybjM putative transport protein YbjL/putative inner membrane protein
RA 987,152 G→T intergenic (‑170/+433) ompF ← / ← asnS outer membrane porin F/asparagine‑‑tRNA ligase
RA 1,333,590 C→A A848E (GCG→GAG)  topA → DNA topoisomerase 1
RA 1,678,604 G→T A60S (GCA→TCA)  ydgH → DUF1471 domain‑containing protein YdgH
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
MC JC 1,978,503 Δ776 bp insB‑5insA‑5 insB‑5, insA‑5
RA 2,065,747 (C)6→5 coding (18/546 nt) cobU ← cobinamide‑P guanylyltransferase/cobinamide kinase
RA 2,173,363 Δ2 bp pseudogene (915‑916/1358 nt) gatC ← galactitol‑specific PTS enzyme IIC component
RA 2,388,147 C→A A94S (GCC→TCC)  yfbN ← uncharacterized protein YfbN
RA 2,995,589 C→A pseudogene (432/707 nt) ygeN ← putative type III secretion system protein
RA 3,012,471 G→T intergenic (+78/+143) yqeA → / ← yqeB putative amino acid kinase YqeA/XdhC‑CoxI family protein YqeB
RA 3,120,156 T→G D375A (GAC→GCC)  glcA ← glycolate/lactate:H(+) symporter GlcA
RA 3,337,239 G→T S6R (AGC→AGA mlaB ← intermembrane phospholipid transport system protein MlaB
RA 3,353,097 A→G intergenic (‑72/+24) arcB ← / ← yhcC sensory histidine kinase ArcB/radical SAM family oxidoreductase YhcC
RA 3,354,487 C→T intergenic (‑437/‑238) yhcC ← / → gltB radical SAM family oxidoreductase YhcC/glutamate synthase subunit GltB
RA 3,560,455 +G pseudogene (151/758 nt) glpR ← DNA‑binding transcriptional repressor GlpR
RA 3,697,013 G→T A186S (GCC→TCC)  bcsE → c‑di‑GMP‑binding protein BcsE
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,835,337 G→T A198E (GCA→GAA)  yicJ ← putative xyloside transporter YicJ
RA 4,261,710 C→A S14R (AGC→AGA dusA → tRNA‑dihydrouridine synthase A
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,321,261 G→T R146S (CGC→AGC)  phnG ← carbon‑phosphorus lyase core complex subunit PhnG
RA 4,394,384 C→A L107M (CTG→ATG)  nnr → NAD(P)HX epimerase/NAD(P)HX dehydratase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3102964 3104068 1105 39 [38] [38] 39 mutY mutY
* * ÷ NC_000913 3423757–3424234 3424526–3424239 6–770 39 [37] [34] 40 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 375203 =NA (NA)48 (0.440) 21/216 3.0 NA noncoding (17/36 nt) other REP31f
?NC_000913 377351 = NA (NA)noncoding (16/37 nt) other REP32a
* ? NC_000913 1207790 =174 (1.240)81 (0.650) 52/244 0.7 36.7% coding (290/630 nt) ycfK e14 prophage; protein StfP
?NC_000913 1209619 = 126 (1.020)pseudogene (37/537 nt) stfE e14 prophage; putative side tail fiber protein fragment
* ? NC_000913 = 12994980 (0.000)237 (1.740) 107/268 0.0 100% intergenic (+253/‑33) ychE/insH21 putative inner membrane protein/IS5 transposase and trans‑activator
?NC_000913 1300698 = 0 (0.000)intergenic (+151/‑484) insH21/oppA IS5 transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 3363001 =199 (1.420)205 (1.470) 87/274 0.1 67.0% coding (195/2382 nt) yhcD putative fimbrial usher protein YhcD
?NC_000913 3766087 = 4 (0.030)coding (3905/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3653230NA (NA)180 (1.280) 92/276 0.1 95.2% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3766089 9 (0.060)coding (3907/4134 nt) rhsA rhs element protein RhsA