breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 21 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,604,3440CT51.1% 33.8 / 124.3 90S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009132,001,9180AC36.4% 94.1 / 44.0 77intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein
*NC_0009134,568,5040TG35.5% 160.2 / 21.9 107T6P (ACC→CCC) mdtMmultidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter
*NC_0009133,556,2050AC31.6% 57.8 / 10.5 38S215R (AGT→AGGrtcARNA 3'‑terminal phosphate cyclase
*NC_0009133,943,4880TG30.0% 49.2 / 12.4 40noncoding (54/76 nt)gltUtRNA‑Glu
*NC_0009134,111,7470GT29.0% 164.4 / 32.2 62N156K (AAC→AAAyiiQDUF1454 domain‑containing protein YiiQ
*NC_0009134,296,0600CT28.7% 234.3 / 95.6 129intergenic (+266/+376)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009134,512,0480AC26.5% 126.4 / 19.6 68L123V (TTG→GTG) fecDferric citrate ABC transporter membrane subunit FecD
*NC_000913888,0140TG26.3% 138.7 / 16.0 76noncoding (44/81 nt)rybBsmall regulatory RNA RybB
*NC_0009133,740,9090TG25.3% 156.7 / 23.4 84V402G (GTG→GGG) avtAvaline‑‑pyruvate aminotransferase
*NC_0009133,206,4350CT24.6% 135.1 / 29.1 65intergenic (‑179/‑28)ttdR/ttdADNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha
*NC_00091329,4960TA24.2% 164.1 / 23.8 66intergenic (+301/‑155)dapB/carA4‑hydroxy‑tetrahydrodipicolinate reductase/carbamoyl phosphate synthetase subunit alpha
*NC_0009133,926,3660AC23.3% 208.2 / 27.1 103G30G (GGT→GGGmioCflavoprotein MioC
*NC_000913753,1250TA22.9% 204.7 / 24.1 83intergenic (‑330/+60)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
*NC_0009132,326,4500TG22.4% 123.2 / 32.9 67G114G (GGT→GGGatoBacetyl‑CoA acetyltransferase
*NC_000913753,1140GT21.9% 230.0 / 18.1 82intergenic (‑319/+71)ybgD/gltAputative fimbrial protein YbgD/citrate synthase
*NC_0009131,491,0980AC21.8% 259.8 / 20.3 124Y66S (TAC→TCC) cybBcytochrome b561
*NC_000913650,8090TG21.7% 119.4 / 15.5 60T350P (ACC→CCC) citCcitrate lyase synthetase
*NC_0009133,404,2150TG21.5% 132.0 / 15.6 65G244G (GGT→GGGyhdHacrylyl‑CoA reductase
*NC_000913646,7470TG21.0% 162.0 / 32.2 81T255P (ACC→CCC) citGtriphosphoribosyl‑dephospho‑CoA synthase

Marginal new junction evidence (lowest skew 10 of 12 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1979646101 (0.880)18 (0.160) 10/276 6.2 15.1% intergenic (‑431/‑107) insA‑5/uspC IS1 protein InsA/universal stress protein C
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 4542690128 (1.120)9 (0.080) 8/258 6.5 7.2% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 113 (1.060)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 4542682 =134 (1.170)5 (0.050) 5/258 7.8 4.0% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 113 (1.060)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 1979646101 (0.880)5 (0.040) 5/276 8.1 4.7% intergenic (‑431/‑107) insA‑5/uspC IS1 protein InsA/universal stress protein C
?NC_000913 = 2067353 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 2030707 =108 (0.940)4 (0.040) 4/260 8.3 3.8% coding (212/471 nt) vsr DNA mismatch endonuclease of the very short patch (VSP) mismatch repair pathway
?NC_000913 = 2030703 100 (0.930)coding (216/471 nt) vsr DNA mismatch endonuclease of the very short patch (VSP) mismatch repair pathway
* ? NC_000913 = 2191800149 (1.300)4 (0.040) 4/264 8.4 2.2% coding (423/543 nt) yehD putative fimbrial protein YehD
?NC_000913 2191946 = 213 (1.940)coding (277/543 nt) yehD putative fimbrial protein YehD
* ? NC_000913 967030 =183 (1.600)3 (0.030) 3/256 8.9 1.8% coding (410/1749 nt) msbA ATP‑binding lipopolysaccharide transport protein
?NC_000913 = 967026 164 (1.540)coding (406/1749 nt) msbA ATP‑binding lipopolysaccharide transport protein
* ? NC_000913 = 323244799 (0.860)5 (0.040) 3/270 9.1 4.9% coding (783/2019 nt) fadH 2,4‑dienoyl‑CoA reductase
?NC_000913 3232453 = 98 (0.870)coding (789/2019 nt) fadH 2,4‑dienoyl‑CoA reductase
* ? NC_000913 = 132708697 (0.850)3 (0.030) 3/272 9.1 3.1% coding (235/876 nt) rluB 23S rRNA pseudouridine(2605) synthase
?NC_000913 1327090 = 90 (0.800)coding (239/876 nt) rluB 23S rRNA pseudouridine(2605) synthase
* ? NC_000913 = 686486122 (1.060)6 (0.050) 3/270 9.1 4.7% coding (184/741 nt) gltJ glutamate/aspartate ABC transporter membrane subunit GltJ
?NC_000913 686491 = 122 (1.090)coding (179/741 nt) gltJ glutamate/aspartate ABC transporter membrane subunit GltJ