breseq version 0.35.4 revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 36 shown, sorted by frequency from high to low) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 4,604,344 | 0 | C | T | 39.1% | 234.9 / 130.8 | 185 | S62F (TCT→TTT) | bglJ | DNA‑binding transcriptional regulator BglJ |
* | NC_000913 | 1,825,061 | 0 | G | A | 34.4% | 96.4 / 34.5 | 61 | intergenic (‑124/+79) | ves/spy | HutD family protein Ves/ATP‑independent periplasmic chaperone |
* | NC_000913 | 4,111,747 | 0 | G | T | 32.0% | 254.4 / 66.1 | 103 | N156K (AAC→AAA) | yiiQ | DUF1454 domain‑containing protein YiiQ |
* | NC_000913 | 4,568,504 | 0 | T | G | 30.6% | 375.8 / 49.8 | 219 | T6P (ACC→CCC) | mdtM | multidrug efflux pump/bile salt:H(+) antiporter/Na(+):H(+) antiporter/K(+):H(+) antiporter |
* | NC_000913 | 2,001,918 | 0 | A | C | 30.6% | 201.5 / 53.0 | 121 | intergenic (‑129/+192) | fliA/fliC | RNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein |
* | NC_000913 | 650,809 | 0 | T | G | 30.5% | 325.6 / 63.2 | 187 | T350P (ACC→CCC) | citC | citrate lyase synthetase |
* | NC_000913 | 2,326,450 | 0 | T | G | 28.7% | 187.8 / 69.4 | 115 | G114G (GGT→GGG) | atoB | acetyl‑CoA acetyltransferase |
* | NC_000913 | 4,296,060 | 0 | C | T | 26.9% | 373.1 / 133.7 | 186 | intergenic (+266/+376) | gltP/yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
* | NC_000913 | 753,125 | 0 | T | A | 26.3% | 272.2 / 45.3 | 114 | intergenic (‑330/+60) | ybgD/gltA | putative fimbrial protein YbgD/citrate synthase |
* | NC_000913 | 4,429,984 | 0 | T | G | 25.9% | 323.3 / 68.3 | 166 | L41V (TTG→GTG) | cycA | serine/alanine/glycine/cycloserine:H(+)symporter |
* | NC_000913 | 4,628,649 | 0 | A | C | 25.4% | 260.8 / 30.4 | 126 | intergenic (+102/+206) | nadR/ettA | DNA‑binding transcriptional repressor/NMN adenylyltransferase NadR/energy‑dependent translational throttle protein EttA |
* | NC_000913 | 1,130,379 | 0 | A | C | 24.3% | 231.2 / 14.6 | 115 | V162G (GTA→GGA) | flgA | flagellar basal body P‑ring formation protein FlgA |
* | NC_000913 | 646,747 | 0 | T | G | 24.3% | 280.1 / 70.8 | 144 | T255P (ACC→CCC) | citG | triphosphoribosyl‑dephospho‑CoA synthase |
* | NC_000913 | 445,558 | 0 | A | C | 24.2% | 239.1 / 56.1 | 124 | V370G (GTA→GGA) | yajR | putative major facilitator superfamily transport protein YajR |
* | NC_000913 | 2,628,625 | 0 | A | C | 24.2% | 191.7 / 24.1 | 91 | V105G (GTC→GGC) | pdeF | cyclic di‑GMP phosphodiesterase PdeF |
* | NC_000913 | 3,556,205 | 0 | A | C | 24.1% | 171.1 / 16.9 | 79 | S215R (AGT→AGG) | rtcA | RNA 3'‑terminal phosphate cyclase |
* | NC_000913 | 3,206,435 | 0 | C | T | 24.0% | 221.4 / 48.5 | 104 | intergenic (‑179/‑28) | ttdR/ttdA | DNA‑binding transcriptional activator Dan/L(+)‑tartrate dehydratase subunit alpha |
* | NC_000913 | 1,825,056 | 0 | G | A | 23.4% | 111.2 / 11.7 | 47 | intergenic (‑119/+84) | ves/spy | HutD family protein Ves/ATP‑independent periplasmic chaperone |
* | NC_000913 | 4,111,752 | 0 | T | C | 23.4% | 265.7 / 26.6 | 124 | K155E (AAA→GAA) | yiiQ | DUF1454 domain‑containing protein YiiQ |
* | NC_000913 | 3,745,397 | 0 | A | C | 23.1% | 285.5 / 19.7 | 143 | L198F (TTA→TTC) | yiaN | 2,3‑diketo‑L‑gulonate:Na(+) symporter ‑ membrane subunit |
Marginal new junction evidence (lowest skew 10 of 58 shown) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 375203 = | NA (NA) | 38 (0.250) | 18/216 | 4.7 | NA | noncoding (17/36 nt) | other | REP31f |
? | NC_000913 | 377351 = | NA (NA) | noncoding (16/37 nt) | other | REP32a | |||||
* | ? | NC_000913 | = 1979489 | 200 (1.010) | 52 (0.260) | 24/276 | 4.8 | 20.6% | intergenic (‑274/‑264) | insA‑5/uspC | IS1 protein InsA/universal stress protein C |
? | NC_000913 | = 2290113 | NA (NA) | noncoding (1/1195 nt) | IS5 | repeat region | |||||
* | ? | NC_000913 | 374921 = | NA (NA) | 33 (0.240) | 15/190 | 4.8 | NA | intergenic (+40/‑538) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377531 = | NA (NA) | intergenic (+220/+4) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 4542682 = | 169 (0.850) | 25 (0.140) | 21/258 | 5.0 | 12.6% | intergenic (+49/‑433) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit |
? | NC_000913 | 4542996 = | 190 (1.030) | intergenic (+363/‑119) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit | |||||
* | ? | NC_000913 | 375102 = | NA (NA) | 29 (0.190) | 16/216 | 5.2 | NA | noncoding (17/36 nt) | other | REP31d |
? | NC_000913 | 377351 = | NA (NA) | noncoding (16/37 nt) | other | REP32a | |||||
* | ? | NC_000913 | = 4542690 | 148 (0.750) | 23 (0.120) | 18/258 | 5.6 | 12.3% | intergenic (+57/‑425) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit |
? | NC_000913 | = 4542986 | 190 (1.030) | intergenic (+353/‑129) | fimE/fimA | regulator for fimA/type 1 fimbriae major subunit | |||||
* | ? | NC_000913 | = 374963 | NA (NA) | 21 (0.150) | 12/190 | 5.6 | NA | noncoding (33/34 nt) | other | REP31a |
? | NC_000913 | = 377487 | NA (NA) | intergenic (+176/+48) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB | |||||
* | ? | NC_000913 | 1979486 = | 199 (1.010) | 60 (0.300) | 16/276 | 6.4 | 23.2% | intergenic (‑271/‑267) | insA‑5/uspC | IS1 protein InsA/universal stress protein C |
? | NC_000913 | 2101749 = | NA (NA) | noncoding (1195/1195 nt) | IS5 | repeat region | |||||
* | ? | NC_000913 | 375001 = | NA (NA) | 15 (0.100) | 8/216 | 7.6 | NA | noncoding (17/36 nt) | other | REP31b |
? | NC_000913 | 377351 = | NA (NA) | noncoding (16/37 nt) | other | REP32a | |||||
* | ? | NC_000913 | 375023 = | NA (NA) | 24 (0.170) | 6/192 | 7.8 | NA | intergenic (+142/‑436) | mhpE/mhpT | 4‑hydroxy‑2‑oxovalerate aldolase/3‑hydroxyphenylpropionate/3‑ hydroxycinnamate:H(+) symporter |
? | NC_000913 | 377530 = | NA (NA) | intergenic (+219/+5) | yaiL/frmB | DUF2058 domain‑containing protein YaiL/S‑formylglutathione hydrolase FrmB |