breseq  version 0.33.1  revision 8505477f25b3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 39,378 C→A D586Y (GAT→TAT)  mecA ← penicillin binding protein 2 prime
RA 478,502 C→A D342E (GAC→GAA ndhF → NADH dehydrogenase (ubiquinone)
RA 584,121 G→T intergenic (+205/‑67) rplA → / → rplJ ribosomal protein L1/ribosomal protein L10
RA 707,909 A→T M1M (TTG→ATG) † mntB ← ABC superfamily ATP binding cassette transporter, ABC protein
RA 973,213 C→A E175* (GAG→TAG)  yitU ← possible HAD superfamily hydrolase
RA 1,002,200 T→G M173R (ATG→AGG)  mecA2 → competence negative regulator MecA
RA 1,105,994 T→C T12T (ACT→ACC pdhD → dihydrolipoyl dehydrogenase
RA 1,196,508 C→A intergenic (+2563/‑1229) ftsQ → / → ylmE cell division protein FtsQ/hypothetical protein
RA 1,232,052 G→T D233Y (GAT→TAT)  prpC → possible PP2C protein phosphatase
RA 1,398,333 G→A Y101Y (TAC→TAT msrA1 ← peptide‑methionine‑(S)‑S‑oxide reductase
RA 1,416,458 A→T I216N (ATT→AAT)  oppD ← oligopeptide ABC superfamily ATP binding cassette transporter, ABC protein
RA 1,471,910 C→T E10038E (GAG→GAA ebh ← extracellular matrix binding protein
RA 1,486,381 G→T Q5215K (CAA→AAA)  ebh ← extracellular matrix binding protein
RA 1,513,930 A→T L413F (TTA→TTT USA300HOU_1379 → hypothetical membrane protein
RA 1,539,250 C→G intergenic (‑349/+37) aroC ← / ← USA300HOU_1405 chorismate synthase/hypothetical protein
RA 1,646,189 G→C A155G (GCA→GGA)  pepP ← M24B family Xaa‑Pro dipeptidase
RA 1,672,590 G→T L135I (CTT→ATT)  rpoD ← DNA‑directed RNA polymerase sigma subunit RpoD
RA 1,744,473 C→T A67T (GCT→ACT)  apt ← adenine phosphoribosyltransferase
RA 2,010,363 C→T C146Y (TGT→TAT)  ygaD ← ABC superfamily ATP binding cassette transporter, membrane protein
RA 2,279,705 G→A intergenic (+1373/+6659) mtlD → / ← glmM mannitol‑1‑phosphate 5‑dehydrogenase/phosphoglucosamine mutase
RA 2,289,324 G→T Q56K (CAG→AAG)  ybbP ← hypothetical membrane protein
RA 2,337,959 C→T intergenic (‑125/‑119) adhR ← / → USA300HOU_2196 possible MerR family transcriptional regulator/hypothetical protein
RA 2,455,692 Δ1 bp coding (1210/1260 nt) USA300HOU_2315 → hypothetical membrane protein
RA 2,455,698 2 bp→AT coding (1216‑1217/1260 nt) USA300HOU_2315 → hypothetical membrane protein
RA 2,532,298 A→T T15T (ACA→ACT murN → FemAB family peptidoglycan biosynthesis protein
RA 2,679,803 G→T G96V (GGA→GTA)  cbpD → possible secretory antigen

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP000730 512620 =103 (1.160)82 (1.320)
+40 bp
56/190 0.1 53.1% intergenic (+688/‑173) recR/rrs1 recombination protein RecR/16S ribosomal RNA
?CP000730 1998278 = NA (NA)intergenic (‑6/+5) USA300HOU_t0050/rrf4 tRNA‑Val/5S ribosomal RNA