breseq  version 0.26.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation freq annotation gene description
MC JC 257,908 Δ776 bp 100% [crl] [crl]
RA 526,792 A→C 21.4% S1178R (AGC→CGC)  rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 526,800 A→G 19.7% E1180E (GAA→GAG rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 1,097,158 C→T 11.4% intergenic (+329/+407) ycdU → / ← serX putative inner membrane protein/tRNA‑Ser
RA 1,287,360 A→T 8.5% intergenic (‑32/+178) tyrV ← / ← tyrT tRNA‑Tyr/tRNA‑Tyr
RA 1,466,138 G→A 5.0% pseudogene (747/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,201 T→C 11.8% pseudogene (810/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,210 G→A 7.3% pseudogene (819/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,258 G→C 6.6% pseudogene (867/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,270 C→T 7.0% pseudogene (879/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,438 T→G 7.6% pseudogene (1047/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,579 G→C 9.6% pseudogene (1188/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,604 G→A 8.0% pseudogene (1213/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,618 T→C 6.8% pseudogene (1227/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,636 T→C 5.8% pseudogene (1245/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,466,711 T→C 9.0% pseudogene (1320/2513 nt) ydbA → pseudogene, autotransporter homolog; interrupted by IS2 and IS30
RA 1,529,175 T→C 100% pseudogene (1274/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,180 T→C 100% pseudogene (1279/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,185 C→T 94.9% pseudogene (1284/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,203 C→A 100% pseudogene (1302/2037 nt) rhsE → pseudogene, Rhs family
RA 1,529,218 C→T 100% pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
RA 1,708,724 G→A 7.2% K602K (AAG→AAA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,844 C→G 5.6% A642A (GCC→GCG rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,708,847 G→A 8.1% E643E (GAG→GAA rsxC → SoxR iron‑sulfur cluster reduction factor component; putative membrane‑associated NADH oxidoreductase of electron transport complex
RA 1,756,457 +A 86.2% coding (760/1413 nt) pykF → pyruvate kinase I
JC JC 1,907,562 IS1 (+) +9 bp 100% coding (22‑30/144 nt) yobF ← DUF2527 family heat‑induced protein
RA 1,915,288 G→C 11.8% L83V (CTT→GTT)  proQ ← RNA chaperone, putative ProP translation regulator
RA 2,173,361 Δ2 bp 100% pseudogene (1‑2/442 nt) gatC ← pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,966,582 C→T 83.6% G618D (GGC→GAC)  ptsP ← PEP‑protein phosphotransferase enzyme I; GAF domain containing protein
RA 3,440,065 G→A 6.8% P322L (CCA→CTA)  rpoA ← RNA polymerase, alpha subunit
RA 3,560,455 +G 100% intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
MC JC 3,815,859 Δ82 bp 100% [rph][rph] [rph], [rph]
RA 3,875,529 C→G 14.5% S31C (TCC→TGC)  yidX → putative lipoprotein
RA 4,296,190 A→G 17.7% intergenic (+396/+246) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,191 A→C 18.3% intergenic (+397/+245) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,268 T→C 33.8% intergenic (+474/+168) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,286 C→T 22.7% intergenic (+492/+150) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,303 A→G 8.0% intergenic (+509/+133) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,304 A→C 13.5% intergenic (+510/+132) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,296,380 +CG 100% intergenic (+586/+56) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1978311 1979738 1428 67 [66] [66] 69 insB1–insA insB1, insA
* * ÷ NC_000913 2033458 2034167 710 70 [64] [64] 68 [yedR]–[yedS] [yedR], [yedS]
* * ÷ NC_000913 2469055 2470731 1677 67 [65] [63] 71 [gtrB]–gtrS [gtrB], gtrS
* * ÷ NC_000913 3769369 3770269 901 67 [65] [66] 67 [yibV]–[yibH] [yibV], yibV, yibU, yibU, [yibH]
* * ÷ NC_000913 3796854 3800422 3569 67 [66] [66] 67 [waaC]–[waaY] [waaC], waaL, waaU, waaZ, [waaY]
* * ÷ NC_000913 4476442 4477245 804 67 [66] [57] 68 yjgL SopA‑central‑domain‑like hexapeptide repeat protein

Unassigned new junction evidence...
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 6300 =141 (1.060)3 (0.020) 3/514 NT 2.2% coding (160/777 nt) yaaA peroxide resistance protein, lowers intracellular iron
?NC_000913 6318 = 130 (1.050)coding (142/777 nt) yaaA peroxide resistance protein, lowers intracellular iron
* ? NC_000913 = 258675NA (NA)4 (0.030) 4/550 NT 2.3% noncoding (1/768 nt) IS1 repeat region
?NC_000913 1771038 = 173 (1.310)intergenic (+67/‑33) ydiL/ydiM putative HTH domain DNA‑binding protein/putative MFS transporter, membrane protein
* ? NC_000913 285874 =119 (0.900)3 (0.020) 3/534 NT 2.5% coding (480/1383 nt) yagG CP4‑6 prophage; putative sugar transporter
?NC_000913 285896 = 117 (0.910)coding (502/1383 nt) yagG CP4‑6 prophage; putative sugar transporter
* ? NC_000913 324960 =138 (1.040)4 (0.030) 4/550 NT 2.8% coding (405/669 nt) ykgH putative inner membrane protein
?NC_000913 1467910 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 1207790 =78 (0.590)61 (0.490) 45/518 NT 45.3% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 74 (0.590)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780576 (0.570)73 (0.580) 57/518 NT 50.1% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 74 (0.590)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 12994980 (0.000)30 (0.230) 22/542 NT 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 1651548NA (NA)4 (0.030) 4/550 NT 2.4% noncoding (706/706 nt) IS2 repeat region
?NC_000913 1755828 = 162 (1.220)coding (131/1413 nt) pykF pyruvate kinase I
* ? NC_000913 = 19785020 (0.000)34 (0.260) 28/534 NT 100% intergenic (‑305/+16) flhD/insB1 flagellar class II regulon transcriptional activator, with FlhC/IS1 transposase B
?NC_000913 1979279 = 0 (0.000)intergenic (‑64/‑474) insA/uspC IS1 repressor TnpA/universal stress protein
* ? NC_000913 2288919 =NA (NA)10 (0.080) 6/550 NT 6.0% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 2365124 158 (1.190)coding (497/603 nt) ais putative LPS core heptose(II)‑phosphate phosphatase
* ? NC_000913 = 2515616NA (NA)3 (0.020) 3/544 NT 2.3% noncoding (1344/1345 nt) IS186 repeat region
?NC_000913 4543608 = 129 (0.980)coding (494/549 nt) fimA major type 1 subunit fimbrin (pilin)
* ? NC_000913 2882234 =132 (1.000)3 (0.020) 3/514 NT 2.4% coding (404/483 nt) casB CRISP RNA (crRNA) containing Cascade antiviral complex protein
?NC_000913 2882252 = 122 (0.990)coding (386/483 nt) casB CRISP RNA (crRNA) containing Cascade antiviral complex protein