breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 56,942 A→G A56A (GCT→GCC lptD ← LPS assembly OM complex LptDE, beta‑barrel component
MC JC 257,908 Δ776 bp [crl] [crl]
RA 300,196 A→G F247L (TTC→CTC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit
RA 323,369 G→A V344V (GTG→GTA ykgF → ferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component
RA 796,454 C→T A198V (GCG→GTG)  modB → molybdate ABC transporter permease; chlorate resistance protein
RA 956,496 A→G V46A (GTA→GCA)  ycaO ← ribosomal protein S12 methylthiotransferase accessory factor
RA 1,055,493 G→A A229V (GCA→GTA)  torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,334,244 G→A S130S (TCG→TCA cysB → N‑acetylserine‑responsive cysteine regulon transcriptional activator; autorepressor
RA 1,529,218 C→T pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
RA 1,968,051 G→A P151S (CCG→TCG)  cheB ← fused chemotaxis regulator: protein‑glutamate methylesterase in two‑component regulatory system with CheA
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,131,557 G→A R172C (CGC→TGC)  wcaC ← putative glycosyl transferase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,230,157 G→A L77L (CTG→TTG)  dusC ← tRNA‑dihydrouridine synthase C
RA 2,526,308 T→C V183V (GTA→GTG yfeR ← transcriptional regulator of yefH
RA 2,810,153 Δ1 bp coding (537/738 nt) ygaZ → putative L‑valine exporter, norvaline resistance protein
RA 3,032,861 (T)5→4 coding (45/126 nt) yqfG → uncharacterized protein
RA 3,041,236 G→A intergenic (‑166/‑77) sdhE ← / → ygfZ flavinator of succinate dehydrogenase; antitoxin of CptAB toxin‑antitoxin pair/iron‑sulfur cluster repair protein, plumbagin resistance
RA 3,168,604 A→G intergenic (‑60/+145) mqsR ← / ← ygiV GCU‑specific mRNA interferase toxin of the MqsR‑MqsA toxin‑antitoxin system; biofilm/motility regulator; anti‑repressor/transcriptional repressor for mcbR biofilm gene
RA 3,270,488 (C)7→8 intergenic (+886/+137) yhaC → / ← garK pentapetide repeats‑related protein/glycerate kinase I
RA 3,292,610 T→C L242L (TTA→TTG rsmI ← 16S rRNA C1402 2'‑O‑ribose methyltransferase, SAM‑dependent
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,667,477 T→C H35R (CAC→CGC)  gadA ← glutamate decarboxylase A, PLP‑dependent
RA 3,672,577 G→A R55Q (CGG→CAG)  yhjC → LysR family putative transcriptional regulator
RA 3,750,962 G→A pseudogene (45/192 nt) ysaD → pseudogene, internal sequence remnant
RA 3,758,369 G→A A498V (GCA→GTA)  selB ← selenocysteinyl‑tRNA‑specific translation factor
RA 3,815,810 Δ1 bp intergenic (‑42/+24) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,951,559 +A pseudogene (18/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
RA 3,964,812 C→T W273* (TGG→TGA rhlB ← ATP‑dependent RNA helicase
RA 3,991,485 T→C C111C (TGT→TGC cyaA → adenylate cyclase
RA 4,015,757 A→G V138A (GTT→GCT)  ysgA ← putative carboxymethylenebutenolidase
RA 4,068,334 G→A Q315* (CAA→TAA)  yihQ ← putative sulpholipid alpha‑glucosidase; alpha‑glucosyl fluoride glucosidase
RA 4,187,507 A→C N720H (AAC→CAC)  rpoC → RNA polymerase, beta prime subunit
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,338,117 (A)8→7 intergenic (‑188/+137) adiY ← / ← adiA adi system transcriptional activator/arginine decarboxylase
RA 4,367,579 A→G V250A (GTG→GCG)  aspA ← aspartate ammonia‑lyase
RA 4,389,327 (C)6→7 coding (44/3324 nt) mscM ← mechanosensitive channel protein, miniconductance
MC JC 4,397,624 (GCTGGC)3→2 coding (213‑218/1848 nt) mutL → methyl‑directed mismatch repair protein
RA 4,475,192 A→G T111A (ACA→GCA)  tabA → biofilm modulator regulated by toxins; DUF386 family protein, cupin superfamily protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423753–3424536 3424536 1–784 67 [66] [66] 67 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)124 (1.030) 107/590 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein