breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 176,134 C→T T343I (ACC→ATC)  clcA → H(+)/Cl(‑) exchange transporter
RA 221,277 G→A I115I (ATC→ATT metI ← DL‑methionine transporter subunit
MC JC 257,908 Δ776 bp [crl] [crl]
RA 268,810 T→C S51G (AGT→GGT)  ykfA ← CP4‑6 prophage; putative GTP‑binding protein
RA 291,529 G→A pseudogene (126/828 nt) yagJ → CP4‑6 prophage; uncharacterized protein;Phage or Prophage Related
RA 300,196 A→G F247L (TTC→CTC)  paoC ← PaoABC aldehyde oxidoreductase, Moco‑containing subunit
RA 323,369 G→A V344V (GTG→GTA ykgF → ferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component
RA 387,569 T→C L200P (CTC→CCC)  tauC → taurine ABC transporter permease
RA 527,078 (G)6→7 coding (3818/4281 nt) rhsD → Rhs protein with DUF4329 family putative toxin domain; putative neighboring cell growth inhibitor
RA 609,147 (C)7→8 coding (1083/1113 nt) insL1 → IS186 transposase
RA 709,028 (A)5→4 coding (695/1407 nt) chiP → chitoporin, uptake of chitosugars
RA 882,192 G→A G181G (GGC→GGT deoR ← deoxyribose‑5‑phosphate‑inducible deoxyribose operon transcriptional repressor; repressor of nupG and tsx
RA 1,055,493 G→A A229V (GCA→GTA)  torS ← hybrid sensory histidine kinase in two‑component regulatory system with TorR
RA 1,089,069 (T)7→8 coding (789/2019 nt) pgaB ← poly‑beta‑1,6‑N‑acetyl‑D‑glucosamine (PGA) N‑deacetylase outer membrane export lipoprotein
RA 1,124,108 (G)6→5 intergenic (‑54/+10) grxB ← / ← mdtH glutaredoxin 2 (Grx2)/multidrug resistance efflux transporter conferring overexpression resistance to norfloxacin and enoxacin
RA 1,415,000 T→C Q796R (CAG→CGG)  recE ← Rac prophage; exonuclease VIII, 5' to 3' specific dsDNA exonuclease
RA 1,529,218 C→T pseudogene (1317/2037 nt) rhsE → pseudogene, Rhs family
RA 1,803,243 G→A pseudogene (150/426 nt) arpB → pseudogene, ankyrin repeats
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 family protein, nitrate‑inducible
MC JC 1,978,503 Δ776 bp insB1insA insB1, insA
RA 2,131,557 G→A R172C (CGC→TGC)  wcaC ← putative glycosyl transferase
RA 2,173,363 Δ2 bp intergenic (‑1/+1) gatC ← / ← gatC pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC/pseudogene, galactitol‑specific enzyme IIC component of PTS;transport; Transport of small molecules: Carbohydrates, organic acids, alcohols; PTS system galactitol‑specific enzyme IIC
RA 2,230,157 G→A L77L (CTG→TTG)  dusC ← tRNA‑dihydrouridine synthase C
RA 2,287,732 G→A pseudogene (1183/2525 nt) yejO ← pseudogene, autotransporter outer membrane homology;putative transport; Not classified; putative ATP‑binding component of a transport system
RA 2,488,076 C→T A365T (GCG→ACG)  yfdE ← acetyl‑CoA:oxalate CoA‑transferase
RA 2,810,153 Δ1 bp coding (537/738 nt) ygaZ → putative L‑valine exporter, norvaline resistance protein
RA 2,860,372 G→A G205R (GGA→AGA)  pphB → serine/threonine‑specific protein phosphatase 2
RA 3,199,319 G→A A108V (GCA→GTA)  glnE ← fused deadenylyltransferase/adenylyltransferase for glutamine synthetase
RA 3,354,228 (T)7→8 intergenic (‑178/‑497) yhcC ← / → gltB putative Fe‑S oxidoreductase, Radical SAM superfamily protein/glutamate synthase, large subunit
RA 3,560,455 +G intergenic (‑2/+1) glpR ← / ← glpR pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon/pseudogene, DNA‑binding transcriptional repressor;regulator; Energy metabolism, carbon: Anaerobic respiration; repressor of the glp operon
RA 3,695,582 T→C pseudogene (404/753 nt) bcsQ ← involved in cellulose production, minD superfamily (pseudogene)
JC JC 3,793,483 IS2 (+) +5 bp coding (197‑201/858 nt) yibB ← YibB family protein, function unknown
RA 3,815,810 Δ1 bp intergenic (‑42/+24) pyrE ← / ← rph orotate phosphoribosyltransferase/ribonuclease PH (defective);enzyme; Degradation of RNA; RNase PH
RA 3,932,937 G→A G541D (GGT→GAT)  kup → potassium transporter
RA 3,951,559 +A pseudogene (18/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
RA 4,126,271 T→C T180T (ACA→ACG priA ← Primosome factor n' (replication factor Y)
RA 4,187,507 A→C N720H (AAC→CAC)  rpoC → RNA polymerase, beta prime subunit
RA 4,200,773 A→G S70G (AGC→GGC)  yjaH → DUF1481 family putative lipoprotein
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate:proton symporter/Sel1 family TPR‑like repeat protein
RA 4,338,117 (A)8→7 intergenic (‑188/+137) adiY ← / ← adiA adi system transcriptional activator/arginine decarboxylase
RA 4,367,579 A→G V250A (GTG→GCG)  aspA ← aspartate ammonia‑lyase
JC 4,397,624 (GCTGGC)3→2 coding (213‑218/1848 nt) mutL → methyl‑directed mismatch repair protein
RA 4,547,814 A→G S25G (AGC→GGC)  fimF → minor component of type 1 fimbriae
RA 4,640,850 C→T intergenic (+308/‑92) yjjY → / → yjtD uncharacterized protein/putative methyltransferase

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423740–3424230 3424523–3424238 9–784 79 [76] [76] 77 [rrfD]–[rrlD] [rrfD], [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 12994980 (0.000)153 (1.150) 126/590 0.0 100% intergenic (+253/‑1684) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 1300698 = 0 (0.000)intergenic (+1453/‑484) ychE/oppA UPF0056 family inner membrane protein/oligopeptide ABC transporter periplasmic binding protein