breseq version 0.29.0 revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Marginal read alignment evidence (highest frequency 20 of 276 shown) | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 436,516 | 0 | C | A | 55.8% | 8.7 / 50.1 | 43 | Q295K (CAA→AAA) | thiL | thiamine monophosphate kinase |
* | NC_000913 | 4,610,466 | 0 | T | G | 50.9% | 66.8 / 89.4 | 53 | G351G (GGT→GGG) | prfC | peptide chain release factor RF‑3 |
* | NC_000913 | 357,890 | 0 | C | A | 50.0% | 22.9 / 22.5 | 40 | L267F (TTG→TTT) | cynR | transcriptional activator of cyn operon; autorepressor |
* | NC_000913 | 941,713 | 0 | T | G | 50.0% | 68.1 / 23.2 | 56 | V252G (GTG→GGG) | dmsA | dimethyl sulfoxide reductase, anaerobic, subunit A |
* | NC_000913 | 3,601,176 | 0 | T | C | 47.7% | 40.8 / 24.6 | 44 | E304G (GAA→GGA) | ftsX | putative ABC transporter permease |
* | NC_000913 | 357,880 | 0 | T | C | 47.4% | 38.9 / 13.0 | 39 | K271E (AAA→GAA) | cynR | transcriptional activator of cyn operon; autorepressor |
* | NC_000913 | 1,222,596 | 0 | G | A | 47.4% | 53.0 / 38.3 | 57 | F13F (TTC→TTT) | ymgD | uncharacterized protein |
* | NC_000913 | 3,443,277 | 0 | A | C | 47.2% | 88.9 / 14.8 | 53 | V274G (GTA→GGA) | secY | preprotein translocase membrane subunit |
* | NC_000913 | 1,312,200 | 0 | G | A | 46.9% | 50.3 / 25.8 | 49 | T16I (ACT→ATT) | yciA | acyl‑CoA esterase |
* | NC_000913 | 3,717,014 | 0 | T | G | 46.9% | 76.4 / 10.1 | 49 | G156G (GGT→GGG) | yiaD | multicopy suppressor of bamB; outer membrane lipoprotein |
* | NC_000913 | 2,252,646 | 0 | T | G | 45.8% | 71.0 / 10.7 | 49 | V316G (GTA→GGA) | yeiI | putative kinase |
* | NC_000913 | 436,497 | 0 | A | C | 45.0% | 58.5 / 10.9 | 41 | V288V (GTA→GTC) | thiL | thiamine monophosphate kinase |
* | NC_000913 | 2,148,330 | 0 | T | G | 44.6% | 108.4 / 14.5 | 65 | G219G (GGT→GGG) | yegD | Hsp70 chaperone family protein |
* | NC_000913 | 1,153,573 | 0 | T | G | 44.1% | 104.6 / 36.9 | 68 | Y92D (TAC→GAC) | pabC | 4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex |
* | NC_000913 | 1,435,727 | 0 | G | A | 43.5% | 92.4 / 27.4 | 62 | intergenic (‑108/+33) | uspF/ompN | stress‑induced protein, ATP‑binding protein/outer membrane pore protein N, non‑specific |
* | NC_000913 | 354,288 | 0 | T | G | 43.5% | 68.8 / 10.5 | 46 | V528G (GTG→GGG) | prpE | propionate‑‑CoA ligase |
* | NC_000913 | 2,265,639 | 0 | A | C | 42.6% | 96.6 / 10.0 | 55 | T64P (ACC→CCC) | yeiP | elongation factor P‑like protein |
* | NC_000913 | 328,803 | 0 | T | G | 42.5% | 65.5 / 30.4 | 47 | T178P (ACC→CCC) | betI | choline‑inducible betIBA‑betT divergent operon transcriptional repressor |
* | NC_000913 | 4,536,197 | 0 | A | T | 42.3% | 68.3 / 37.1 | 52 | intergenic (+166/+417) | yjhR/nanS | pseudogene, helicase family; putative frameshift suppressor/9‑O‑acetyl N‑acetylneuraminic acid esterase |
* | NC_000913 | 366,526 | 0 | T | G | 42.1% | 107.8 / 11.2 | 57 | T329P (ACC→CCC) | lacI | lactose‑inducible lac operon transcriptional repressor |
Marginal new junction evidence (sorted from low to high skew) | |||||||||||
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seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | 4542682 = | 57 (0.930) | 3 (0.050) | 3/280 | 11.6 | 4.9% | intergenic (+49/‑433) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) |
? | NC_000913 | 4542996 = | 62 (1.080) | intergenic (+363/‑119) | fimE/fimA | tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin) |