breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 250 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,001,9180AC49.1% 91.5 / 11.9 59intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
*NC_0009132,378,9680CT46.7% 17.6 / 21.1 46E98K (GAA→AAA) menD2‑succinyl‑5‑enolpyruvyl‑6‑hydroxy‑3‑ cyclohexene‑1‑carboxylate synthase; SEPHCHC synthase
*NC_0009133,665,7020CA46.1% 83.5 / 54.5 76A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
*NC_0009132,619,2300TC46.0% 65.1 / 11.5 50I311V (ATT→GTT) uraAuracil permease
*NC_000913646,7470TG45.9% 91.9 / 13.9 61T255P (ACC→CCC) citG2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
*NC_0009131,736,7890GC45.5% 62.2 / 13.2 44L168V (CTG→GTG) ydhPputative MFS transporter, inner membrane protein
*NC_0009133,595,0440TA45.2% 40.8 / 21.0 42Y146F (TAC→TTC) livMbranched‑chain amino acid ABC transporter permease
*NC_000913689,5140AC43.2% 56.1 / 11.5 37G456G (GGT→GGGlntapolipoprotein N‑acyltransferase
*NC_000913589,9340TG42.9% 50.7 / 10.6 36T341P (ACC→CCC) nfrAbacteriophage N4 receptor, outer membrane subunit
*NC_000913599,5760TG42.1% 87.1 / 26.4 57V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
*NC_0009132,984,4410AC41.5% 95.3 / 17.8 54G384G (GGT→GGGyqeFshort chain acyltransferase
*NC_000913925,9000AG41.2% 87.5 / 14.1 52noncoding (72/88 nt)serWtRNA‑Ser
*NC_0009132,337,7230AC40.8% 75.3 / 12.5 50G566G (GGT→GGGgyrADNA gyrase (type II topoisomerase), subunit A
*NC_0009131,574,0510GT40.7% 93.3 / 22.1 54V384V (GTC→GTApqqLputative periplasmic M16 family zinc metalloendopeptidase
*NC_0009132,532,9880GA40.5% 53.7 / 18.9 43G194S (GGC→AGC) cysKcysteine synthase A, O‑acetylserine sulfhydrolase A subunit
*NC_000913646,7770GT40.4% 68.5 / 27.8 52Q245K (CAA→AAA) citG2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
*NC_0009133,508,6620TA40.4% 81.1 / 19.5 57E384D (GAA→GATyhfWphosphopentomutase‑related metalloenzyme superfamily protein
*NC_0009133,026,9410AC40.0% 89.8 / 10.6 50E392D (GAA→GACguaDguanine deaminase
*NC_0009134,067,7450AG39.7% 99.8 / 11.5 58L511P (CTG→CCG) yihQputative sulpholipid alpha‑glucosidase; alpha‑glucosyl fluoride glucosidase
*NC_0009132,676,1060CA39.6% 70.8 / 25.4 53L240F (TTG→TTTyphDputative sugar ABC transporter permease

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 7806310 (0.000)22 (0.420) 16/258 4.2 53.0% intergenic (+2/‑134) lysT/valT tRNA‑Lys/tRNA‑Val
?NC_000913 2521487 = 39 (0.740)intergenic (+159/+106) lysV/xapR tRNA‑Lys/transcriptional activator of xapAB
* ? NC_000913 = 7806310 (0.000)21 (0.400) 11/258 5.9 41.6% intergenic (+2/‑134) lysT/valT tRNA‑Lys/tRNA‑Val
?NC_000913 781811 = 59 (1.120)intergenic (+159/‑274) lysQ/nadA tRNA‑Lys/quinolinate synthase, subunit A
* ? NC_000913 = 454269075 (1.230)3 (0.050) 3/280 11.1 3.9% intergenic (+57/‑425) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 = 4542986 78 (1.360)intergenic (+353/‑129) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 4542682 =81 (1.330)3 (0.050) 3/280 11.1 3.7% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542996 = 78 (1.360)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
* ? NC_000913 1615598 =67 (1.100)3 (0.050) 3/286 11.3 4.8% coding (795/882 nt) yneJ putative DNA‑binding transcriptional regulator
?NC_000913 = 1625301 54 (0.920)intergenic (+10/+34) ydeJ/dcp inactive PncC family protein/dipeptidyl carboxypeptidase II