breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 381 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,8800TC57.5% 16.8 / 47.8 40K271E (AAA→GAA) cynRtranscriptional activator of cyn operon; autorepressor
*NC_0009131,478,3240TG54.7% 52.1 / 88.2 53G33G (GGT→GGGynbBputative CDP‑diglyceride synthase
*NC_000913357,8900CA51.3% 6.4 / 40.4 37L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
*NC_0009131,413,7350AT50.6% 57.5 / 133.5 79Y2* (TAT→TAAydaCDUF1187 family protein, Rac prophage; putative double‑strand break reduction protein
*NC_000913357,8750AT48.8% 35.7 / 21.2 43N272K (AAT→AAAcynRtranscriptional activator of cyn operon; autorepressor
*NC_000913941,7130TG48.4% 81.8 / 25.1 63V252G (GTG→GGG) dmsAdimethyl sulfoxide reductase, anaerobic, subunit A
*NC_0009131,506,0380AC46.8% 101.4 / 12.9 64F15V (TTT→GTT) ydcOBenE family inner membrane putative transporter
*NC_0009133,206,4350CT44.9% 73.9 / 40.3 69intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
*NC_000913354,2880TG44.1% 91.5 / 19.2 59V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NC_0009131,376,5050AC43.9% 84.2 / 22.9 57T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
*NC_0009134,302,6580TG43.6% 87.0 / 13.4 56T141P (ACC→CCC) mdtOmembrane translocase (MDR) of MdtNOP efflux pump, PET family
*NC_0009134,538,1090AC41.9% 135.4 / 27.2 74G219G (GGT→GGGnanMN‑acetylneuraminic acid mutarotase
*NC_0009134,378,8020AC41.7% 102.9 / 17.2 60V48G (GTA→GGA) ampCpenicillin‑binding protein; beta‑lactamase, intrinsically weak
*NC_0009132,303,6720CA40.7% 92.8 / 29.2 55intergenic (‑175/‑233)napF/ecoferredoxin‑type protein, role in electron transfer to periplasmic nitrate reductase NapA/ecotin, a serine protease inhibitor
*NC_0009131,222,5960GA40.7% 76.5 / 31.3 59F13F (TTC→TTTymgDuncharacterized protein
*NC_0009131,413,7510AC40.5% 105.1 / 29.8 75N59K (AAT→AAGralRRac prophage; restriction alleviation protein
*NC_000913345,3280CA40.5% 124.7 / 43.3 84intergenic (+337/‑76)yahL/yahMuncharacterized protein/uncharacterized protein
*NC_0009131,621,1180AC40.4% 88.1 / 10.3 52V7G (GTG→GGG) eamAcysteine and O‑acetyl‑L‑serine efflux system
*NC_000913599,5760TG39.1% 131.5 / 14.5 65V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
*NC_0009131,097,5810AG38.9% 130.8 / 15.3 72noncoding (72/88 nt)serXtRNA‑Ser

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 925901 =65 (0.900)19 (0.260)
+C
15/296 7.4 25.5% noncoding (71/88 nt) serW tRNA‑Ser
?NC_000913 = 1097399 47 (0.650)intergenic (+570/+166) ycdU/serX putative inner membrane protein/tRNA‑Ser
* ? NC_000913 1207790 =81 (1.120)10 (0.150) 10/266 8.6 13.2% coding (290/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 1209619 = 59 (0.910)pseudogene (1/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related
* ? NC_000913 = 120780586 (1.190)4 (0.060) 4/266 12.2 5.6% coding (305/630 nt) stfP e14 prophage; uncharacterized protein
?NC_000913 = 1209602 59 (0.910)pseudogene (18/501 nt) stfE pseudogene, e14 prophage; side tail fiber protein fragment family;Phage or Prophage Related