breseq  version 0.29.0  revision 8f9c342918e4
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 704 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913357,8900CA51.3% 25.4 / 58.8 37L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
*NC_0009134,604,3440CT50.0% 42.5 / 42.6 64S62F (TCT→TTT) bglJbgl operon transcriptional activator
*NC_000913354,2880TG49.3% 90.7 / 39.3 75V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NC_0009131,376,5050AC48.5% 86.9 / 29.0 68T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
*NC_0009131,174,6100AC48.0% 111.0 / 19.0 77W186G (TGG→GGG) ycfTinner membrane protein
*NC_0009133,443,2770AC46.6% 102.6 / 28.4 74V274G (GTA→GGA) secYpreprotein translocase membrane subunit
*NC_0009131,174,6050TC45.7% 114.4 / 20.4 81G187G (GGA→GGGycfTinner membrane protein
*NC_0009132,434,8820TG45.3% 109.8 / 29.5 75Y252S (TAC→TCC) truAtRNA pseudouridine(38‑40) synthase
*NC_0009134,580,6400CT45.3% 71.1 / 20.8 54E275K (GAA→AAA) hsdSspecificity determinant for hsdM and hsdR
*NC_0009131,245,2340GT45.2% 85.0 / 50.0 73L25F (TTG→TTTycgYuncharacterized protein
*NC_000913345,3280CA44.7% 101.1 / 51.0 76intergenic (+337/‑76)yahL/yahMuncharacterized protein/uncharacterized protein
*NC_0009133,585,0860AC44.4% 126.7 / 17.2 81Y580D (TAC→GAC) ggtgamma‑glutamyltranspeptidase
*NC_0009131,312,2130GA44.1% 72.5 / 42.1 69L12F (CTT→TTT) yciAacyl‑CoA esterase
*NC_0009132,483,2540GA43.2% 50.2 / 19.9 45S29L (TCA→TTA) emrKmultidrug resistance efflux pump membrane fusion protein
*NC_0009131,679,3040AC43.1% 99.5 / 20.8 72Y293S (TAC→TCC) ydgHDUF1471 family periplasmic protein
*NC_0009132,001,9180AC42.7% 140.2 / 15.8 75intergenic (‑129/+192)fliA/fliCRNA polymerase, sigma 28 (sigma F) factor/flagellar filament structural protein (flagellin)
*NC_0009133,177,7480CA42.5% 84.3 / 40.8 73E55* (GAG→TAG) nudFADP‑ribose pyrophosphatase
*NC_0009131,911,7540TG42.1% 125.9 / 13.1 76T275P (ACC→CCC) htpXputative endopeptidase
*NC_000913646,7470TG41.9% 129.5 / 15.2 74T255P (ACC→CCC) citG2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase
*NC_0009133,995,4570TG41.8% 112.4 / 15.7 68G232G (GGT→GGGdapFdiaminopimelate epimerase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4542682 =44 (0.540)6 (0.080) 6/280 13.0 9.5% intergenic (+49/‑433) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)
?NC_000913 4542996 = 73 (0.950)intergenic (+363/‑119) fimE/fimA tyrosine recombinase/inversion of on/off regulator of fimA/major type 1 subunit fimbrin (pilin)