breseq  version 0.27.1  revision 87c22d663cc3
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 1337 shown)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,386,8620GA60.8% 33.7 / 104.2 97intergenic (+31/‑72)yfbL/yfbMputative M28A family peptidase/DUF1877 family protein
*NC_0009133,665,6980TC57.3% 33.9 / 178.8 144D38G (GAT→GGT) gadXacid resistance regulon transcriptional activator; autoactivator
*NC_0009133,665,7020CA56.9% 55.0 / 190.4 144A37S (GCG→TCG) gadXacid resistance regulon transcriptional activator; autoactivator
*NC_0009133,686,0280TC54.9% 75.6 / 127.2 93E1049G (GAG→GGG) bcsCcellulose synthase subunit
*NC_000913354,2880TG53.8% 91.5 / 124.4 78V528G (GTG→GGG) prpEpropionate‑‑CoA ligase
*NC_000913357,8900CA53.6% ‑0.5 / 58.1 56L267F (TTG→TTTcynRtranscriptional activator of cyn operon; autorepressor
*NC_0009133,813,2720CT51.2% 35.0 / 97.7 82S181L (TCG→TTG) dfpfused 4'‑phosphopantothenoylcysteine decarboxylase/phosphopantothenoylcysteine synthetase, FMN‑binding
*NC_000913357,8800TC50.8% 55.3 / 103.2 65K271E (AAA→GAA) cynRtranscriptional activator of cyn operon; autorepressor
*NC_0009132,332,8230AG50.8% 119.3 / 192.4 132pseudogene (1580/4605 nt)yfaSpseudogene, bacterial alpha2‑macroglobulin YfaS variant family; putative membrane protein
*NC_0009133,640,9810CT49.5% 47.0 / 72.6 100F40F (TTC→TTTdtpBdipeptide and tripeptide permease B
*NC_0009131,356,2010TG48.6% 99.2 / 20.2 73T304P (ACC→CCC) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
*NC_0009133,133,3020TG48.4% 105.5 / 47.0 97H219P (CAC→CCC) yghSputative ATP‑binding protein
*NC_000913657,6760AC48.2% 147.8 / 40.4 115V88G (GTG→GGG) flcfluoride efflux channel, dual topology membrane protein
*NC_0009133,206,4350CT48.1% 53.8 / 82.6 104intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
*NC_0009131,222,2330GA46.0% 54.1 / 43.6 75pseudogene (60/75 nt)ycgHpseudogene; putative ATP‑binding component of a transport system
*NC_0009132,571,0360TG45.7% 93.6 / 15.0 71N239T (AAC→ACC) eutEaldehyde oxidoreductase, ethanolamine utilization protein
*NC_000913540,5580TG45.6% 104.5 / 44.3 92intergenic (+50/‑7)allB/ybbYallantoinase/putative uracil/xanthine transporter
*NC_000913364,5260CA45.5% 111.3 / 53.8 100G594C (GGC→TGC) lacZbeta‑D‑galactosidase
*NC_0009133,443,2770AC45.1% 126.0 / 50.5 91V274G (GTA→GGA) secYpreprotein translocase membrane subunit
*NC_0009133,686,0210AC45.1% 135.9 / 15.0 91G1051G (GGT→GGGbcsCcellulose synthase subunit

Marginal new junction evidence (lowest skew 10 of 194 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 381689NA (NA)21 (0.200) 13/278 6.1 NA noncoding (430/1331 nt) IS2 repeat region
?NC_000913 = 381743 NA (NA)noncoding (484/1331 nt) IS2 repeat region
* ? NC_000913 381292 =NA (NA)12 (0.120) 9/278 7.7 NA noncoding (33/1331 nt) IS2 repeat region
?NC_000913 381312 = NA (NA)noncoding (53/1331 nt) IS2 repeat region
* ? NC_000913 = 2756112112 (1.020)9 (0.090) 8/274 8.0 7.7% intergenic (+734/‑47) smpB/intA tmRNA‑binding trans‑translation protein/CP4‑57 prophage; integrase
?NC_000913 = 2756129 111 (1.090)intergenic (+751/‑30) smpB/intA tmRNA‑binding trans‑translation protein/CP4‑57 prophage; integrase
* ? NC_000913 3381839 =89 (0.810)9 (0.090) 8/278 8.1 9.4% coding (1097/1368 nt) degQ serine endoprotease, periplasmic
?NC_000913 3381869 = 89 (0.860)coding (1127/1368 nt) degQ serine endoprotease, periplasmic
* ? NC_000913 = 2547356119 (1.080)11 (0.110) 8/278 8.1 9.0% coding (684/1425 nt) murP N‑acetylmuramic acid permease, EIIBC component, PTS system
?NC_000913 = 2547371 112 (1.090)coding (699/1425 nt) murP N‑acetylmuramic acid permease, EIIBC component, PTS system
* ? NC_000913 3060398 =88 (0.800)9 (0.090) 8/280 8.2 9.9% coding (646/894 nt) argP transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
?NC_000913 3060457 = 80 (0.770)coding (705/894 nt) argP transcriptional regulator for arginine transport and DNA replication genes; replication initiation inhibitor
* ? NC_000913 = 2169291115 (1.050)8 (0.080) 8/280 8.2 6.6% coding (580/900 nt) yegS phosphatidylglycerol kinase, metal‑dependent
?NC_000913 = 2169311 118 (1.140)coding (600/900 nt) yegS phosphatidylglycerol kinase, metal‑dependent
* ? NC_000913 2765420 =122 (1.110)5 (0.060) 4/208 8.3 6.3% intergenic (‑267/+93) yfjL/yfjM CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein/CP4‑57 prophage; uncharacterized protein
?NC_000913 = 2765411 64 (0.830)intergenic (‑258/+102) yfjL/yfjM CP4‑57 putative defective prophage, DUF4297/DUF1837 polymorphic toxin family protein/CP4‑57 prophage; uncharacterized protein
* ? NC_000913 3592584 =NA (NA)7 (0.070) 7/268 8.4 7.3% noncoding (21/99 nt) RIP258 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP258 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
?NC_000913 3592648 = 89 (0.900)noncoding (85/99 nt) RIP258 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site RIP258 (repetitive extragenic palindromic) element; contains 2 REP sequences and 1 IHF site
* ? NC_000913 3927418 =105 (0.960)8 (0.080) 7/272 8.5 7.6% coding (264/993 nt) asnA asparagine synthetase A
?NC_000913 3927457 = 98 (0.970)coding (303/993 nt) asnA asparagine synthetase A