Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F29 I1 R1
|
57 |
65.8 |
2790598 |
98.6% |
2751529 |
146.7 |
Breseq alignment
N/A
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑G‑‑T‑‑G‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAAC > NC_002947_CJ‑RC/1070101‑1070384
|
GACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCTATCTTAAGAATGCTCGCCAGGCCATGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑G‑‑T‑‑G‑‑AG < NB501016:282:H2V7GAFX2:4:11409:5027:4176/149‑1 (MQ=60)
CGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑G‑‑T‑‑G‑‑Agatcg < NB501016:282:H2V7GAFX2:4:21512:17530:14166/151‑6 (MQ=60)
ATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑G‑‑TGAG‑‑ATCGAGCGCCGC > NB501016:282:H2V7GAFX2:4:11502:8943:14640/1‑151 (MQ=60)
CGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑G‑‑TGAG‑‑ATCGAGCGCCGCGCGGGCG < NB501016:282:H2V7GAFX2:4:11507:11305:14103/148‑1 (MQ=60)
GTTCCCGTCATGTATCGCTGTGCTGTGTGTGGCGTTCAAGCTTAAGAAGGCTCGCCAGGCCTAGGGCTGATCGTGTTTGTTCGTAAGGGGTATTCCGTTAGGTTTTCGGCGCA‑‑T‑‑G‑‑TGAG‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATC < NB501016:282:H2V7GAFX2:3:21401:4429:1141/148‑1 (MQ=60)
GGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑G‑‑TGAG‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATCT > NB501016:282:H2V7GAFX2:4:11512:2402:6536/1‑138 (MQ=60)
ggctgggctgtggggggctttcaaggtgaccacggcgcgcccgcccaaggcgTGATCGGGTTTGTTCGTACGGGGTAGGCCGTTAGGTTTGCGGCGCC‑‑T‑‑G‑‑T‑‑GACATCGCGCGCCGGGCGGGCGGCGCGCGATCTCACAGGCGATGAAAGCC < NB501016:282:H2V7GAFX2:3:21508:8245:18958/99‑1 (MQ=60)
cggggctgtggggggcttccacgcggaagacgccccgcccggcaacGGGCTGAGCGGGTTTGTGCGTACGGGGTAGGCCTTTAGGTTTTCGGCGCC‑‑TGGG‑‑C‑‑G‑‑ACCGCCCGCCGCGCGGGCGGCGCGCGATCTCACAGGCGATGAAAGCCTA < NB501016:282:H2V7GAFX2:4:21603:10340:9999/105‑1 (MQ=40)
gcggggggtggcttaaacGCTGAAGAAGGCGCGCCAGGCCAAGGGCTGACCGGGTTTGTTCTTACGGGGTAGGCGTTAAGGTTTCGGGCGCC‑‑T‑‑G‑‑TGAG‑‑ATCCCCCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGG < NB501016:282:H2V7GAFX2:4:21505:7189:5495/133‑1 (MQ=35)
gggggggcttccccgctcaccAAGGCTCGCCAGGCCACGGGCTGACCGGGTTGGTCCGTAAGGGGTATCCCGTTAGGTTTCCGGCCCC‑‑T‑‑GGCA‑‑G‑‑CCCGCGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATG < NB501016:282:H2V7GAFX2:2:11206:7823:9735/130‑1 (MQ=47)
gggggcttccacgctcaccAAGGCCCGCCCGGCCATGGGCTTATCGGGTTTCTTCGAACGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑G‑‑TGAG‑‑ACCGACCGCCGCGGGGCCGGCGCGCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCG < NB501016:282:H2V7GAFX2:1:11104:9074:16506/132‑1 (MQ=60)
GGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑G‑‑TGAG‑‑ATCGAGCGCCGCGCGGGCG > NB501016:282:H2V7GAFX2:3:21401:9638:4722/1‑111 (MQ=60)
TCAAGCTGAAGAAGGCGCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑G‑‑TGAG‑‑ACCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCG < NB501016:282:H2V7GAFX2:1:11204:4702:18664/150‑1 (MQ=60)
acaaaggcccccccGGCCACGGGCTGATCGGTTTTGTTCGTACGGGGTAGGCCTTTTGGTTTTCGGCGCC‑‑T‑‑G‑‑T‑‑GACACCGCCCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACG < NB501016:282:H2V7GAFX2:1:11303:12216:17743/137‑1 (MQ=60)
CGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑TGGG‑‑A‑‑G‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCT < NB501016:282:H2V7GAFX2:1:21205:14052:18489/151‑1 (MQ=60)
GGGCTGATCGGGTTTGTTCGTACGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑G‑‑TGAG‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCC < NB501016:282:H2V7GAFX2:3:21406:11545:1763/150‑1 (MQ=60)
TATCGGGTTTGTGGGTAAGGGGTTGGCCGTTCGGTTTTCGGCGCCGGT‑‑C‑‑T‑‑T‑‑ATCTTGCGCCGCGCGGGGGGGGCTGGATCTCAAAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGACCCTGACGCGTTCCCCTTCCATGCCCCTCAAT < NB501016:282:H2V7GAFX2:4:11607:8879:13243/150‑1 (MQ=60)
GTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑G‑‑TGAG‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAG < NB501016:282:H2V7GAFX2:4:11610:21865:6941/151‑1 (MQ=60)
TATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑G‑‑TGAG‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCAC < NB501016:282:H2V7GAFX2:1:21212:23789:8248/150‑1 (MQ=60)
GCCGTTAGGTTTTCGGCGCC‑‑T‑‑G‑‑TGAG‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTG < NB501016:282:H2V7GAFX2:4:21612:8355:14119/151‑1 (MQ=60)
CGGCGCC‑‑T‑‑G‑‑TGAG‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAAC < NB501016:282:H2V7GAFX2:3:11605:17914:4106/150‑1 (MQ=60)
|
GACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑G‑‑T‑‑G‑‑ATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCAAC > NC_002947_CJ‑RC/1070101‑1070384
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
