Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A3 F14 I1 R1
|
57 |
86.1 |
3667460 |
98.7% |
3619783 |
146.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
1,070,248 |
+GA |
intergenic (+48/+94) |
aroP‑I → / ← PP_0928 |
aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 1,070,246 | 1 | . | G | 85.7%
| 89.1
/ 11.8
| 36 | intergenic (+46/+96) | aroP‑I/PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
| Reads supporting (aligned to +/- strand): ref base . (1/4); new base G (7/23); total (9/27) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.88e-01 |
| * | NC_002947_CJ‑RC | 1,070,246 | 2 | . | A | 86.1%
| 88.7
/ 12.5
| 36 | intergenic (+46/+96) | aroP‑I/PP_0928 | aromatic amino acid transport protein/K+‑dependent Na+/Ca+ exchanger‑like protein |
| Reads supporting (aligned to +/- strand): ref base . (1/4); new base A (8/23); total (9/27) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01 |
CCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCA > NC_002947_CJ‑RC/1070098‑1070382
||
ccTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑t‑‑gt < 4:144162/149‑1 (MQ=255)
tGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑t‑‑gt > 5:123142/1‑147 (MQ=255)
aCGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GT‑‑Ga < 2:131220/147‑1 (MQ=255)
aTCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCgcgg < 4:146718/149‑1 (MQ=255)
aTCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCgcgg < 2:231502/149‑1 (MQ=255)
gTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTTGCTTTCAATCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCGTT‑‑GA‑‑GATCGAGCGCCGCGCGGgcggcg < 1:224047/149‑1 (MQ=255)
aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCt < 6:123142/148‑1 (MQ=255)
aTGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCt < 2:44806/148‑1 (MQ=255)
aTGCTGATCCCGGCATGGATCGCTGTGCGGTGGGTGACTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTc < 4:48901/149‑1 (MQ=39)
tGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCAGT‑‑GC‑‑GATAGAGCGCCCCGCCGGGGGGGCTc > 8:448944/1‑148 (MQ=255)
cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGa < 2:22930/148‑1 (MQ=255)
cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAt < 6:331890/149‑1 (MQ=255)
cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCCCGCGCGGCGCTCGa < 5:182754/148‑1 (MQ=38)
cTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCCCGCGCGGCGCTCGAt < 6:119338/149‑1 (MQ=38)
cTGATCCCGGCAGGGATCGCTGTGCTGTGGGGGGCTTTCAAGCTGAAGAAGGCGCGCCAGGCCAAGGGCTGATCGGGTTTGTGCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAt < 2:202811/149‑1 (MQ=38)
tCCCGGCATGTATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGCTTGTTCGTAAGGGGTATGCCGTTAGTTTTTCGGCGCC‑‑T‑‑GTGAGATCGACCGCCGCGCGGGCGGCGCTCGAtct < 7:315071/147‑1 (MQ=38)
tCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATAGAGCGCCGCGCGGGCGGGGCTCGAtgtga > 6:324064/1‑145 (MQ=255)
cccGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTc > 6:85886/1‑141 (MQ=255)
cccGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTc < 5:85886/141‑1 (MQ=255)
ggATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCGCGATCGAACAGGCGAt > 6:130140/1‑148 (MQ=38)
ggATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCCCCCCGCGGGCGGGGCTCGAAGTAACAGGCGAt > 4:228267/1‑148 (MQ=255)
aagaagGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGGTCGATCGGACAGGCGGTGAAAGCCTAAAGGCATGGGTCTGGCGGcccggc > 6:279129/1‑146 (MQ=38)
ggCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAt > 8:110953/1‑99 (MQ=37)
gCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGAtc < 7:110953/99‑1 (MQ=37)
gCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTcc < 2:429497/147‑1 (MQ=255)
ccAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCt < 2:173241/149‑1 (MQ=255)
aaGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTTAGATCGAGCGCCGCGCGGGCGGAGCTCGATCTCACAGTCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCGTCCATGc > 2:248998/1‑149 (MQ=39)
aGGGGTGATCGGGTTTGTTCTTATGGGGTTTGCCGTTTGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGGGCGGGCGGCGCTCTATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGcc < 6:301107/149‑1 (MQ=38)
aTCGGGTTTGTTCGTACGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCaa < 2:145667/148‑1 (MQ=255)
ttCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCCGGT‑‑GA‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTc < 8:146631/147‑1 (MQ=255)
ttCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTcc < 6:230776/148‑1 (MQ=255)
cGTACGGGGTAGGCGGTTTGGTTTTCGGCGCC‑‑TGGGC‑‑GAGCGCGCGCCGGGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGcc < 3:177304/149‑1 (MQ=38)
gTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGcc < 7:129219/148‑1 (MQ=255)
gTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATAGAGCGCCGCGCGGGCGGCGCTCGATATAACAGGCGAAGAAAGCCGAAAGGCATTCGTATGGCGGCCCGGGCGCGGTGCCCGTCCAAGCCCCGCAAATCTGCCTCCGCCAAgcacgc > 6:344533/1‑149 (MQ=19)
cGTTAGTTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGGGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg < 5:425513/149‑1 (MQ=38)
cGTTAGGTTTTCGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAgtg < 2:14135/149‑1 (MQ=255)
ggTTTTCGGCGCC‑‑T‑‑GTGAGATCGCGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGt < 5:328596/97‑1 (MQ=25)
cGGCGCC‑‑T‑‑GTGAGATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCa < 2:415038/148‑1 (MQ=255)
||
CCTGACGCCGCCGATTCGCATCTCGGTAATGCTGATCCCGGCATGGATCGCTGTGCTGTGGGTGGCTTTCAAGCTGAAGAAGGCTCGCCAGGCCAAGGGCTGATCGGGTTTGTTCGTAAGGGGTATGCCGTTAGGTTTTCGGCGCC‑‑T‑‑GT‑‑GATCGAGCGCCGCGCGGGCGGCGCTCGATCTCACAGGCGATGAAAGCCTAAAGGCATGCGTCTGGCGGCCCTGACGCGTTCCCCTTCCATGCCCCTCAATGCTGCCTCCGCCAAGCACGCACAGTGGTGTACAGCA > NC_002947_CJ‑RC/1070098‑1070382
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
