Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A4 F13 I0 R1
|
140 |
52.5 |
2164708 |
98.8% |
2138731 |
146.1 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
freq |
annotation |
gene |
description |
| RA |
NC_002947_CJ‑RC |
4,768,005 |
T→A |
100% |
intergenic (+112/‑70) |
fpvA → / → tpaK |
TonB‑dependent outer membrane ferripyoverdine receptor FpvA/probable terephthalate transporter, MFS superfamily protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | NC_002947_CJ‑RC | 4,768,005 | 0 | T | A | 100.0%
| 28.8
/ NA
| 10 | intergenic (+112/‑70) | fpvA/tpaK | TonB‑dependent outer membrane ferripyoverdine receptor FpvA/probable terephthalate transporter, MFS superfamily protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base A (8/2); total (8/2) |
| Rejected as polymorphism: Frequency below/above cutoff threshold. |
| Rejected as polymorphism: Variant not supported by required number of reads on each strand. |
ACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTAATTACAATTAATCATCGGCTCGTTTAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCA > NC_002947_CJ‑RC/4767862‑4768142
|
aCTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATaa > 3:153519/1‑147 (MQ=255)
gACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTc > 7:204901/1‑146 (MQ=255)
tGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCata > 6:38243/1‑147 (MQ=255)
ttttttGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTcc > 1:184442/1‑147 (MQ=255)
gAGGTCAGGTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCgg > 6:217574/1‑147 (MQ=255)
ggTATGATTACTATTGACAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCa < 4:153519/146‑1 (MQ=255)
tgaCAATTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAg > 3:24837/3‑147 (MQ=255)
aTTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGt > 6:194430/1‑147 (MQ=255)
aTTAATCATCGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGt < 8:204901/147‑1 (MQ=255)
tcGGCTCGTATAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCa > 2:163372/1‑147 (MQ=255)
|
ACTTCACCATCACCGCCAAGTACAGCTTCTGATCGCCTGACGTTGAACGCAAAAAACCGCACCCAGGTGCGGTTTTTTGAATTCACTAGTAGTCTCTATGGAGGTCAGGTATGATTACTAATTACAATTAATCATCGGCTCGTTTAATGTGATCAGACCTGGAATTGTGAGCGGATAACAATTCTTAAGATTAACTCACACAGGAGATATCATATGTCCTTAGCACCATCGCGCGTGACACTGCCGGATTTCATCGATTCGCGGCCAGTGAGTCGATACCA > NC_002947_CJ‑RC/4767862‑4768142
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
N/A
