Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A10 F11 I3 R1	74	850.3	37733610	99.3%	37469474	136.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	499,203	A→G	intergenic (+47/‑249)	uhpA CDS (DNA‑binding response regulator UhpA) → / → PP_0411 CDS (Polyamine ABC transporter_ ATP‑binding protein)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	499,203	0	A	G	99.6%	1025.9 / NA	259	intergenic (+47/‑249)	uhpA CDS (DNA‑binding response regulator UhpA)/PP_0411 CDS (Polyamine ABC transporter_ ATP‑binding protein)	–/–
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (45/213); total (45/214)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 259 total aligned reads displayed.

CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCT‑CCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGG  >  AE015451_phaM1/499072‑499331

ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                               <  2:7090261/137‑1 (MQ=255)

ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCAGGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                               <  1:9057847/137‑1 (MQ=255)

 cacaAGGCACGCCTGATGAAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATg                                                                                                                              <  2:8079141/137‑1 (MQ=255)

   caAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGcc                                                                                                                            >  2:14444440/1‑137 (MQ=255)

   caAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCACGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGcc                                                                                                                            >  2:18721735/1‑137 (MQ=255)

    aaGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGTCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGccc                                                                                                                           <  1:16099390/137‑1 (MQ=255)

      ggcgggCCTGATGCAGAAGGTGGAAGTGACCTCGATGGCGGAGTTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATGGCGACATACGGGGGCACGAGAATTGCCGAAGCCGATGTGGGAGCGGGCAGGCCtgc                                                                                                                         <  2:11392403/132‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAATTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:16841044/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGTTGGTGAAGGACGCCATGGAGCACAAGCTGGTCGGATTGCGACAGACGGGTGCCAGAAATGTGCCGAAGCCTATGGGGGAGCGGGCATGCCcgc                                                                                                                         <  1:7855724/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:3890955/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:10338213/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:6712495/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:8153757/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:12459051/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:16607306/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:17574881/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:2419771/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:3745522/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:4463880/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:6268785/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:7478873/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:98340/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:10175470/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:14830475/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:16933728/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:14178852/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:1550518/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:11217569/137‑1 (MQ=255)

           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACg                                                                                                                   <  1:5496384/137‑1 (MQ=255)

           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACg                                                                                                                   <  2:2837994/137‑1 (MQ=255)

           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACg                                                                                                                   <  2:56916/137‑1 (MQ=255)

           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACg                                                                                                                   <  2:7578407/137‑1 (MQ=255)

           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACGTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACg                                                                                                                   <  2:8743729/137‑1 (MQ=255)

           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGGGCCCGAAACTGGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACg                                                                                                                   <  2:8154594/137‑1 (MQ=255)

             cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCG‑AACagg                                                                                                                 >  1:17346241/1‑137 (MQ=255)

             cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCACG‑AACagg                                                                                                                 >  2:1971711/1‑137 (MQ=255)

              tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGggc                                                                                                                <  1:12723670/137‑1 (MQ=255)

              tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGggc                                                                                                                <  1:1802593/137‑1 (MQ=255)

              tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACAggc                                                                                                                >  2:10262712/1‑137 (MQ=255)

               gATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAACGCCCGCG‑AACgggac                                                                                                               >  2:14641001/1‑135 (MQ=255)

                aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGggcct                                                                                                              <  2:18130977/137‑1 (MQ=255)

                aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGggcct                                                                                                              <  1:14372309/137‑1 (MQ=255)

                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGggcctggc                                                                                                           <  1:17691037/137‑1 (MQ=255)

                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGggcctggc                                                                                                           <  1:475715/137‑1 (MQ=255)

                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGggcctggc                                                                                                           <  2:11916299/137‑1 (MQ=255)

                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCACA‑AACAggaccggc                                                                                                           >  1:17866749/1‑137 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGANCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGgcctggcctg                                                                                                        <  1:15251063/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCATAAGCTGATCTGATTGCGACATACGGGGGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGgcctggcctg                                                                                                        <  2:1393677/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGgcctggcctg                                                                                                        <  2:3840430/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGgcctggcctg                                                                                                        <  1:13200714/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGgcctggcctg                                                                                                        <  2:14932689/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGgcctggcctg                                                                                                        <  2:4619331/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGgcctggcctg                                                                                                        <  2:6510658/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGgcctggcctg                                                                                                        <  1:18574898/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGgcctggcctg                                                                                                        <  2:12091292/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGgcctggcctg                                                                                                        <  1:5345499/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGgcctggcctg                                                                                                        <  1:4830867/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGgcctggcctg                                                                                                        <  1:3281961/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGgcctggcctg                                                                                                        <  2:13453701/137‑1 (MQ=255)

                      aaGTTGAAAGCGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGGGTACAAGCTGATCTGATGGCGACATACGGGTGCCCGAAACGGGCCGAAGCCTAGGTGGGAGCGGGAGTGCCAGCG‑AACGGgcctggcctg                                                                                                        <  1:2346592/137‑1 (MQ=255)

                      aaGTGGAAAGGGAACTCGCTGGTGGAGCTGGTGAAGTACGCCAGGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGgcctggcctg                                                                                                        <  1:12012003/137‑1 (MQ=255)

                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCctgct                                                                                                      <  2:14057297/137‑1 (MQ=255)

                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCctgct                                                                                                      <  2:2842151/137‑1 (MQ=255)

                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCctgct                                                                                                      <  2:4091367/137‑1 (MQ=255)

                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCctgct                                                                                                      <  1:1920338/137‑1 (MQ=255)

                           gAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGAt                                                                                                   <  2:10127286/137‑1 (MQ=255)

                            aaaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGAtc                                                                                                  <  1:14488106/137‑1 (MQ=255)

                            aaaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGAtc                                                                                                  <  2:16245382/137‑1 (MQ=255)

                            aaaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGACATGCCCGCG‑AACGGGCCTGGCCTGCTGAtc                                                                                                  >  2:12960362/1‑137 (MQ=255)

                              aGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGANCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGTCCGCG‑AACGGGCCTGGCCTTCTGAtctc                                                                                                >  1:15894311/1‑137 (MQ=255)

                               gTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGTATGCCCCCG‑AACAGGCCCGGCCCGATGATctct                                                                                               >  2:15400931/1‑137 (MQ=255)

                                tGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTa                                                                                              <  2:4553984/137‑1 (MQ=255)

                                tGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTa                                                                                              <  1:13834103/137‑1 (MQ=255)

                                tGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTa                                                                                              <  1:5670495/137‑1 (MQ=255)

                                tGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTa                                                                                              <  2:534165/137‑1 (MQ=255)

                                     tCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTATGGCt                                                                                         <  1:9442414/137‑1 (MQ=255)

                                          ggCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTATGGCTGAGcc                                                                                    <  2:18395146/137‑1 (MQ=255)

                                               aGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGAGGGCCGAG‑AAAGGGAATGGGATACTGGTCTCTATGGCTTGGCCTGccc                                                                               >  1:6329284/1‑136 (MQ=255)

                                                gCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAATTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctc                                                                              <  2:18361265/137‑1 (MQ=255)

                                                gCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctc                                                                              <  2:12653195/137‑1 (MQ=255)

                                                gCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctc                                                                              <  2:15904818/137‑1 (MQ=255)

                                                gCTGGTGAAGTACGCCATGGAGCACAAGCTGATCGGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctc                                                                              <  2:15018475/137‑1 (MQ=255)

                                                 cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGGTTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctct                                                                             <  2:13616470/137‑1 (MQ=255)

                                                 cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctct                                                                             <  2:10543616/137‑1 (MQ=255)

                                                 cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctct                                                                             <  2:15522100/137‑1 (MQ=255)

                                                    gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCGCA‑GACAGGCCCGGGCCGATGAGTTCTCCGGCTGAGACAGACTCTTcg                                                                          >  2:13784096/1‑137 (MQ=255)

                                                         gTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGc                                                                     <  2:15301806/137‑1 (MQ=255)

                                                             gcgagggAGCACAAGCTGATTTGATTGCGAAAGACGGGTGCCCGAAACTTGCCGAAGCCTGTGTGGGAGCGGGAAGGCCCTCG‑AATGGGCCTGGCCGGCGGATCTCTAGGGTTGAGCCGGCCTCTTCGCGGGCATGc                                                                 <  2:9085241/132‑1 (MQ=255)

                                                                 tGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAACGTCCTCG‑AACAGGGCCGGCCCGCTGACCTCTATTGCTTGGGCAGCATCTTTTCGGGCACGccccc                                                             >  2:14254225/1‑135 (MQ=255)

                                                                    aGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGAGGCCCGCGAAAAGGACCGGTCCTTTTG‑TTTTTCTGGTTTAGCCTGCCTCCTCTCGGGCGTGCCCCCT‑cc                                                         >  1:15680541/1‑137 (MQ=255)

                                                                    aGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCCCA‑AACAGGCCCCGCCTGCTTATCCCTCTGGCCGAACCGGCCTCTTCCCAAGCCTGCCCGcc‑cc                                                         >  1:16681932/1‑137 (MQ=255)

                                                                     gCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGACCGCG‑AACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCT‑ccc                                                        >  2:9639313/1‑137 (MQ=255)

                                                                         aaGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGGTCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCAGCT‑CCCACAg                                                    >  1:10686131/1‑137 (MQ=255)

                                                                              gatctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAACGCCCCCA‑AACGGGACTGGCCTTCTGATCTCTCTGGCTGAGACGGCCCCCTTTCGGGCATGCCCCCTCCCCCCAGGttt                                                >  2:957715/1‑137 (MQ=255)

                                                                                   gatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAACGACCCCG‑AAAACACCTTGCCCGCCTCTCTCTATTGCTTAGCCTACCTCTTCGTAAGCATGTCCACG‑CCCCCAGATGTCCTggg                                          >  2:3523825/1‑136 (MQ=255)

                                                                                             gaagggTGCCGGAACTTTGCTGAAGCTGGTGTGGGGGTGGGCGGGCCAGAG‑AACGGGCCTGTCTTGCTGCTCTCTATGGTTGATCTGGCTTCTTCGCGGGCATCCCCGCT‑CCCACAGGTTTCGTGGTTGGCTCGCaa                                <  2:2246961/134‑1 (MQ=255)

                                                                                                        gAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCACG‑AACGAGACTCGACCGCCTATCCCTCTAGGCGAGCAAAACCCTTCCCGGGCAATCCCCCT‑CCCCCAGGTTTCGTGGTTTGCTCCCAAAGGGACTCCaa                     >  2:1007559/1‑137 (MQ=255)

                                                                                                             ttGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCT‑CCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCg                <  1:2826766/137‑1 (MQ=255)

                                                                                                             gtccagAAGCCTGTGTGGGGGAGGGCGTTCCCGGA‑ACCGGGCCTGCCCTTCGGGTCTCGGGGTCTGAGCCGGTCTCTTCGGGGGACTCCCCGCT‑CCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCg                <  2:8374312/132‑1 (MQ=255)

                                                                                                              ggCCGAAGCCTGTGGGGGGGAGGGCGTGCCCGGG‑AAGGGGCCTGGCTTGTGGGTCTCTATGGTGGAGTCGGCCTTTTCGTGGGCGTTCCCGCT‑CCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGc               <  2:5940516/136‑1 (MQ=255)

CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCG‑AACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCT‑CCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGG  >  AE015451_phaM1/499072‑499331

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

AGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  AE015451_phaM1/499055‑499353

AGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG                                                                                                                                                      >  LH00941:114:22FVMWLT1:2:1165:7943:8830/1‑151 (MQ=60)

AGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG                                                                                                                                                      >  LH00941:114:22FVMWLT1:2:2218:16975:24763/1‑151 (MQ=60)

 GCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1152:49934:15492/151‑1 (MQ=60)

 GCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGC                                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1152:49925:15476/151‑1 (MQ=60)

    AGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATG                                                                                                                                                  >  LH00941:114:22FVMWLT1:2:2211:49240:11633/1‑151 (MQ=60)

      ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2162:31046:7918/151‑1 (MQ=60)

      ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1169:2997:12882/151‑1 (MQ=60)

      ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2246:2692:4058/151‑1 (MQ=60)

      ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1207:46236:9375/151‑1 (MQ=60)

      ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2234:43287:10384/151‑1 (MQ=60)

      ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1253:40975:1705/151‑1 (MQ=60)

      ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2214:41900:9903/151‑1 (MQ=60)

      ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2135:24917:20872/151‑1 (MQ=60)

      ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2162:31055:7902/151‑1 (MQ=60)

      ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGGACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1162:22476:7549/151‑1 (MQ=60)

            AGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAA                                                                                                                                          <  LH00941:114:22FVMWLT1:2:2122:8757:4315/151‑1 (MQ=60)

             GCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC                                                                                                                                         <  LH00941:114:22FVMWLT1:2:1220:25656:12545/151‑1 (MQ=60)

             GCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC                                                                                                                                         <  LH00941:114:22FVMWLT1:2:1220:25647:12561/151‑1 (MQ=60)

                CCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAACAGG                                                                                                                                      >  LH00941:114:22FVMWLT1:2:2248:9099:19127/1‑151 (MQ=60)

                CCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCACGCCCACGAAAGGG                                                                                                                                      >  LH00941:114:22FVMWLT1:2:2262:13545:20584/1‑151 (MQ=60)

                 CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtcccacat                                                                                                                                     <  LH00941:114:22FVMWLT1:2:2235:7148:15460/151‑9 (MQ=60)

                 CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCAGGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtcccacat                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1265:15607:1320/151‑9 (MQ=60)

                  CACAATGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGACCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCC                                                                                                                                    <  LH00941:114:22FVMWLT1:2:2224:31444:23402/151‑1 (MQ=60)

                    CAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtcccacatagg                                                                                                                                  <  LH00941:114:22FVMWLT1:2:2256:35558:7405/151‑12 (MQ=60)

                     AAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGTCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtcccacataggc                                                                                                                                 <  LH00941:114:22FVMWLT1:2:2230:17410:8766/151‑13 (MQ=60)

                     AAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtcccacataggc                                                                                                                                 <  LH00941:114:22FVMWLT1:2:1111:4726:26701/151‑13 (MQ=60)

                      AGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAACGGGgccggt                                                                                                                                >  LH00941:114:22FVMWLT1:2:2256:46365:10175/1‑145 (MQ=60)

                      AGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCacaaacaggaccggc                                                                                                                                >  LH00941:114:22FVMWLT1:2:2256:46384:10175/1‑136 (MQ=60)

                       GTCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2251:23188:25980/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAATTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAATTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2239:43860:3626/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTTGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGAAATGCGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2237:35844:18790/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGTTGGTGAAGGACGCCATGGAGCACAAGCTGGTCGGATTGCGACAGACGGGTGCCAGAAATGTGCCGAAGCCTATGGGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1247:9968:10448/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAGGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1136:25508:12065/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1101:6473:5131/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1128:36834:27581/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1124:12621:5628/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2127:44618:13778/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1251:13656:21512/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1251:13638:21512/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2111:10938:23946/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2114:17493:8270/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2211:44683:14114/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1230:19795:17125/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1158:31638:22906/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1143:31194:21336/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1122:6815:14275/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2242:26451:17349/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2201:31250:22073/151‑1 (MQ=60)

                       GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGGGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1266:14350:3562/151‑1 (MQ=60)

                          ACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGC                                                                                                                            <  LH00941:114:22FVMWLT1:2:2212:29641:19831/151‑1 (MQ=60)

                            GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:1212:36408:20343/151‑1 (MQ=60)

                            GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:1202:16439:9583/151‑1 (MQ=60)

                            GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:2112:37314:7053/151‑1 (MQ=60)

                            GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCGGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:1212:36399:20327/151‑1 (MQ=60)

                               TGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATC                                                                                                                       <  LH00941:114:22FVMWLT1:2:2120:9247:4139/151‑1 (MQ=60)

                               TGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATC                                                                                                                       <  LH00941:114:22FVMWLT1:2:2149:41493:29054/151‑1 (MQ=60)

                               TGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATC                                                                                                                       <  LH00941:114:22FVMWLT1:2:1127:5456:26012/151‑1 (MQ=60)

                                 ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGGTTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                                     <  LH00941:114:22FVMWLT1:2:2128:7231:6284/151‑1 (MQ=60)

                                 ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                                     <  LH00941:114:22FVMWLT1:2:2204:2525:18982/151‑1 (MQ=60)

                                 ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGANCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGTCCGCGAACGGGCCTGGCCTTCTGATCTC                                                                                                                     >  LH00941:114:22FVMWLT1:2:2227:21515:7709/1‑151 (MQ=60)

                                    CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:1108:19582:5323/151‑1 (MQ=60)

                                    CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:2253:32757:21801/151‑1 (MQ=60)

                                    CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:2253:32747:21785/151‑1 (MQ=60)

                                       atgaatgcacgagtctcgtgggctcggagatgtgtataagagacaGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1261:20812:22890/106‑1 (MQ=60)

                                       aagtgtaagGTGAACTCGTTGGCGGAGCGGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCTGAAGCCGATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2245:26045:3802/142‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTCCGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2205:28069:23354/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGANCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2217:19508:26172/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGANCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2217:45302:21272/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2266:29105:21817/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2227:35077:17333/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2205:28060:23370/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2227:35096:17333/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2266:29114:21833/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2157:40790:4939/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1135:23484:5932/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1136:36695:20680/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1149:21394:20952/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1149:48408:25115/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1153:2812:5964/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1201:20914:1833/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1202:21746:23354/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1208:45709:23194/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1210:11170:13586/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1217:5844:14451/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1247:37342:3802/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1254:35243:23482/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2155:36242:15540/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2155:36233:15556/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGACCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1203:45561:18710/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCAGGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2245:26035:3786/151‑1 (MQ=60)

                                       AAGTTGAAAGTGAACTCGCTGGCGGAGCGGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2245:26017:3786/151‑1 (MQ=60)

                                       AAGTTGAAAGCGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGGGTACAAGCTGATCTGATGGCGACATACGGGTGCCCGAAACGGGCCGAAGCCTAGGTGGGAGCGGGAGTGCCAGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1135:15829:27101/151‑1 (MQ=60)

                                       AAGTGGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCAGGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1129:43490:7533/151‑1 (MQ=60)

                                       AAGTGGAAAGGGAACTCGCTGGTGGAGCTGGTGAAGTACGCCAGGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCGCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2139:15089:10896/151‑1 (MQ=60)

                                         GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTTCGACATACGGGTGCCCGAAACTTGCCGAAGCCTAGGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCGG                                                                                                             <  LH00941:114:22FVMWLT1:2:2116:50655:27213/151‑1 (MQ=60)

                                         GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                                             <  LH00941:114:22FVMWLT1:2:1129:42991:18614/151‑1 (MQ=60)

                                         GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                                             <  LH00941:114:22FVMWLT1:2:2106:22051:27117/151‑1 (MQ=60)

                                         GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                                             <  LH00941:114:22FVMWLT1:2:2116:50646:27197/151‑1 (MQ=60)

                                             AAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCC                                                                                                         <  LH00941:114:22FVMWLT1:2:2206:46458:11360/151‑1 (MQ=60)

                                                 TGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1215:51339:8030/151‑1 (MQ=60)

                                                 TGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1215:51321:8030/151‑1 (MQ=60)

                                                 TGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1215:51358:8030/151‑1 (MQ=60)

                                                 TGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2166:2951:14723/151‑1 (MQ=60)

                                                 TGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2166:6140:17461/151‑1 (MQ=60)

                                                      TCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTC                                                                                                <  LH00941:114:22FVMWLT1:2:1270:7749:20952/151‑1 (MQ=60)

                                                           GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                                           <  LH00941:114:22FVMWLT1:2:1160:30122:25371/151‑1 (MQ=60)

                                                           GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                                           <  LH00941:114:22FVMWLT1:2:1225:6233:28574/151‑1 (MQ=60)

                                                            GCGGAGCTGGTGAAGTATGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGC                                                                                          <  LH00941:114:22FVMWLT1:2:2257:44137:16789/151‑1 (MQ=60)

                                                            GCGGAGCTGGTGAAGTATGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGC                                                                                          <  LH00941:114:22FVMWLT1:2:2216:37074:12497/151‑1 (MQ=60)

                                                            GCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGACCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGC                                                                                          <  LH00941:114:22FVMWLT1:2:2201:5558:4443/151‑1 (MQ=60)

                                                                  CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                                    <  LH00941:114:22FVMWLT1:2:2203:35410:17109/151‑1 (MQ=60)

                                                                  CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                                    <  LH00941:114:22FVMWLT1:2:1216:29576:26540/151‑1 (MQ=60)

                                                                  CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCGGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                                    <  LH00941:114:22FVMWLT1:2:2223:30695:14867/151‑1 (MQ=60)

                                                                            ACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGGTCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCAGCTCCCACAG                                                                          >  LH00941:114:22FVMWLT1:2:2119:27552:15219/1‑151 (MQ=60)

                                                                                                           ACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCTCGAACaaggcttgtcttctgagcttttttgctggggtgggctccttttaggggttgccccttcccccaggtttgttggtttttcctcaaggaagttccc                                           >  LH00941:114:22FVMWLT1:2:1222:40273:7277/1‑57 (MQ=60)

                                                                                                                              TTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGG                        <  LH00941:114:22FVMWLT1:2:1142:40180:29247/151‑1 (MQ=60)

                                                                                                                                                    GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCTCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTCCAACAAATCTTCC  >  LH00941:114:22FVMWLT1:2:1231:30113:19751/1‑151 (MQ=60)

                                                                                                                                                    GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  LH00941:114:22FVMWLT1:2:2160:25213:25227/1‑151 (MQ=60)

AGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  AE015451_phaM1/499055‑499353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`