Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A14 F35 I1 R1	74	857.2	37938082	99.4%	37710453	136.3

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	499,203	A→G	intergenic (+47/‑249)	uhpA CDS (DNA‑binding response regulator UhpA) → / → PP_0411 CDS (Polyamine ABC transporter_ ATP‑binding protein)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	499,203	0	A	G	100.0%	859.0 / NA	216	intergenic (+47/‑249)	uhpA CDS (DNA‑binding response regulator UhpA)/PP_0411 CDS (Polyamine ABC transporter_ ATP‑binding protein)	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (44/172); total (44/172)

Only 100 of 216 total aligned reads displayed.

CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGATCTCTATGGCTGAGCCGGCCTCTTCGC‑‑GGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGC  >  AE015451_phaM1/499072‑499332

ccACAAGGAACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTTGCGGAGCTGGTGAATTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                                  <  2:17238897/137‑1 (MQ=255)

  acaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATgg                                                                                                                                >  1:9256085/1‑136 (MQ=255)

  acaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGc                                                                                                                                <  2:17488629/137‑1 (MQ=255)

    aaGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGccc                                                                                                                              <  1:6303244/137‑1 (MQ=255)

    aaGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGtcc                                                                                                                              >  1:17264884/1‑137 (MQ=255)

     aGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCTGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcg                                                                                                                             <  1:16240166/137‑1 (MQ=255)

     aGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGccca                                                                                                                             >  1:9155751/1‑136 (MQ=255)

     aGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcg                                                                                                                             >  1:16088967/1‑137 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  1:17698944/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  1:5775245/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  2:6889266/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  1:17899340/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  1:2266025/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  2:5938753/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  1:5222789/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  2:5631795/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  1:7861142/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  1:8095635/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  2:10130213/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  2:17781923/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  2:14831066/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  2:15126484/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  2:9664792/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  1:10555619/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  2:7097262/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  1:16417231/137‑1 (MQ=255)

      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                            <  2:9580993/137‑1 (MQ=255)

      ggCACGCATGATGCAGAAGTTGAAAGTGAACTCGTTGGCGGAGTTAGTGAAATACGCCATTGAGCACAAGCTGATCTGATTGCGACATATGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCtgc                                                                                                                            <  2:16723759/137‑1 (MQ=255)

           gcttGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                      <  2:12132793/134‑1 (MQ=255)

           gcatGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCAAGGGAGCACAAGCTGATCGGATTGCGACAGACGGGTGCCCGAAACTTGCCGAAGCTTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                      <  1:797118/134‑1 (MQ=255)

           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCTATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                      <  2:5014749/137‑1 (MQ=255)

           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                      <  2:7377430/137‑1 (MQ=255)

           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                      <  1:14860185/137‑1 (MQ=255)

           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                      <  1:8572477/137‑1 (MQ=255)

            ccTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑gg                                                                                                                     <  2:2667340/137‑1 (MQ=255)

             cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAAC‑agg                                                                                                                    >  1:3685661/1‑137 (MQ=255)

             cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAAA‑ggg                                                                                                                    >  2:3086129/1‑137 (MQ=255)

             cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCCaaaagg                                                                                                                     >  2:16119980/1‑133 (MQ=255)

              tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggc                                                                                                                   <  1:11507606/137‑1 (MQ=255)

               gATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAAC‑gagac                                                                                                                  >  1:13690377/1‑133 (MQ=255)

               gATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCACGAAC‑gggac                                                                                                                  >  2:6578390/1‑135 (MQ=255)

                aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAAC‑aggacc                                                                                                                 >  2:11228809/1‑134 (MQ=255)

                aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATTCCCGCGAAC‑Gggcct                                                                                                                 <  1:15655286/137‑1 (MQ=255)

                aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcct                                                                                                                 <  2:15198020/137‑1 (MQ=255)

                 tGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctg                                                                                                                <  2:17773927/137‑1 (MQ=255)

                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGGGGGCCGCGAAA‑ggggatggt                                                                                                              >  1:3456990/1‑131 (MQ=255)

                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                              <  1:11290304/137‑1 (MQ=255)

                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                              <  1:10013311/137‑1 (MQ=255)

                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                              <  2:1835723/137‑1 (MQ=255)

                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCAAGAAC‑gggaatgga                                                                                                              >  2:13794777/1‑131 (MQ=255)

                     gAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggcct                                                                                                            <  1:12153469/137‑1 (MQ=255)

                      aaGTTGAAAGTGACCCCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  2:17679910/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  1:4274168/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  2:825444/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  2:17097657/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  1:11908432/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  2:18947583/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  2:2019897/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  1:9654556/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  2:8534300/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  2:8439288/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  1:14699096/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  1:6190753/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  1:5245168/137‑1 (MQ=255)

                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  1:15491579/137‑1 (MQ=255)

                      aaGTTGAAAGCGAACTCGCTGGCGGGGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  2:16655193/137‑1 (MQ=255)

                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTTATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCctg‑ct                                                                                                        <  2:16371408/137‑1 (MQ=255)

                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCctg‑ct                                                                                                        <  2:644081/137‑1 (MQ=255)

                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCctg‑ct                                                                                                        <  2:10737876/137‑1 (MQ=255)

                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCctg‑ct                                                                                                        <  2:7171638/137‑1 (MQ=255)

                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCctg‑ct                                                                                                        <  1:17764744/137‑1 (MQ=255)

                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCctg‑ct                                                                                                        <  2:10737918/137‑1 (MQ=255)

                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCGGATCTGATTGCGACATACGGGTGCCCGAAACTGGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCctg‑ct                                                                                                        <  1:17571569/137‑1 (MQ=255)

                            aaaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGAtc                                                                                                    <  1:9975970/137‑1 (MQ=255)

                                tGAACTCGCTGGCGGAGCGGGGGAAGTACGCCATGGAGCACAAGCTGATCGGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGATCTCTa                                                                                                <  2:11949712/137‑1 (MQ=255)

                                tGAACTCGCCGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGATCTCTa                                                                                                <  2:7998305/137‑1 (MQ=255)

                                  aaCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGATCTCTATg                                                                                              <  1:13786901/137‑1 (MQ=255)

                                     tCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                              >  2:6259427/1‑119 (MQ=255)

                                            cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGATCTCTATGGCTGAGCCgg                                                                                    <  1:3374374/137‑1 (MQ=255)

                                                gCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGATCTCTATGGCTGAGCCGGCctc                                                                                <  1:7124532/137‑1 (MQ=255)

                                                gCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGATCTCTATGGCTGAGCCGGCctc                                                                                <  1:13614823/137‑1 (MQ=255)

                                                 cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGATCTCTATGGCTGAGCCGGCctct                                                                               <  1:16548126/137‑1 (MQ=255)

                                                 cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGATCTCTATGGCTGAGCCGGCctct                                                                               <  1:17625496/137‑1 (MQ=255)

                                                 cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGATCTCTATGGCTGAGCCGGCctct                                                                               <  1:17625533/137‑1 (MQ=255)

                                                      gAAGTACGCAAGGGAGCAAAAGCTGATCTGATTGCGACAGACGGGTGCCCAAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCAAAC‑GGGCCTGGCCTG‑CTGATCTCTGTGGCGGAGCCGGCCTTTTcgc‑‑g                                                                        <  2:10329606/137‑1 (MQ=255)

                                                       aaGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGATCTCTATGGCTGAGCCGGCCTCTTCGC‑‑gg                                                                       <  1:14477775/137‑1 (MQ=255)

                                                            cGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCACGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCCCGAAC‑GGGACCGGCATGACTGGT‑TCTCTGGGTGAGCCAACCTCCTCCC‑‑GAGCagg                                                                  >  2:7155314/1‑135 (MQ=255)

                                                                    aGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGTCCGCGCACAGGAACTGTCTTT‑TTG‑TCTCTCTGGTTGAGCCGTCCTCTTCCC‑‑GGGCCCGCCCGcccc                                                          >  1:10449499/1‑137 (MQ=255)

                                                                                         gACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAAC‑GGGCCCGGCCCG‑CCGGTTTCTCCGGATGGGACAGCACCCTCCC‑‑GGGCATACCCACTCCCACAAGTTTCGTGGTTGGTTc                                     >  2:4461521/1‑137 (MQ=255)

                                                                                         gACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCGCGAAC‑AAGAATGGAACG‑CTGAGCTCTCTGGCTGGGCCAGCACCCCCGC‑‑GGGTATTCCCACCCCCCCCAGTTTCGTTGTTGGCTc                                     >  1:18225720/1‑137 (MQ=255)

                                                                                            aTACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAA‑GGGCCTTGCCTC‑CTGAGTTCTCTGGCTGGGTCAGCCTCCTCGT‑‑GGGCGTTTTCTCTCCCCCAAGTTTCGTGGTTGGCTTGCa                                  >  2:17059618/1‑137 (MQ=255)

                                                                                             gatgggTGCCAGAAACTTGCGGAAGCTGGTGGGGGGGGGGGCGTGCCTGGAAGT‑GGGCTGGGCCGG‑CGGATCTCGGGGGTTGAGCTGTCCTCTTCGT‑‑GGGCGTCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCaa                                 <  2:3790682/134‑1 (MQ=255)

                                                                                               cGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCaa                                 <  2:11365509/137‑1 (MQ=255)

                                                                                               cGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCaa                                 <  2:12870483/137‑1 (MQ=255)

                                                                                                      ccGAAACTTGTTGAAGCCGGTGTGGGGGAGGGCATGCCTGGGAAC‑GGTCCTGGCCTG‑CTGATCTCTATGGCTGAGCCGGTCTCTTCGT‑‑GGGCGTGCCCGCTCCCAAAGGTTTCGTGGTTGGCTCGCAAGGGCATTcc                        <  1:18685468/137‑1 (MQ=255)

                                                                                                        gAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGTCCACGAAC‑GGGACTTGTCTT‑CTGGGCTCTATTGTTGAGGCAGCCTCTTTTC‑‑TGGCATGCCCCCTCCCCCAAGTTTTTTTGTTGGTTCTCCAGGGAATTcccc                      >  2:1152096/1‑135 (MQ=255)

                                                                                                        gAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                           <  2:10157343/55‑1 (MQ=255)

                                                                                                           gcTTGCGGAAGCCTGTGGGGGAGAGGGCGTGCCCGCGAAC‑GGGCCGGGTCTG‑CGGACCTCGAGGGCGGAGCCGGCCTCTTCGT‑‑GGGCGTGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATcc                   <  2:14147267/136‑1 (MQ=255)

                                                                                                             ttcccGAAGCCGGTGGGGGAGAGGGCATGCCTGGGAAT‑GGGCTTGGCTTG‑CGGATCTCTATTGTTGAGCCGTCCTCTTCGG‑‑GGACATTCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCg                 <  2:6827438/134‑1 (MQ=255)

                                                                                                             ttGCCGAATCCGGTGGGGGAGCGGGCGTGCCCGTAAAT‑GGGTCTGGCTTG‑CTGGTCTCTAGGGCGGAGCTGTCCTCTTCGC‑‑GGGTATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCg                 <  2:18678070/137‑1 (MQ=255)

CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAAC‑GGGCCTGGCCTG‑CTGATCTCTATGGCTGAGCCGGCCTCTTCGC‑‑GGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGC  >  AE015451_phaM1/499072‑499332

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

GAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  AE015451_phaM1/499054‑499353

GAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGG                                                                                                                                                       >  LH00941:114:22FVMWLT1:2:2142:43814:5019/1‑151 (MQ=60)

GAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGG                                                                                                                                                       >  LH00941:114:22FVMWLT1:2:1253:1545:14883/1‑151 (MQ=60)

      GACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGAGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGC                                                                                                                                                 >  LH00941:114:22FVMWLT1:2:1267:50858:21576/1‑151 (MQ=60)

       ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGNCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2249:31647:6813/151‑1 (MQ=60)

       ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2266:36113:20696/151‑1 (MQ=60)

       ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2149:25314:13330/151‑1 (MQ=60)

       ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1212:20664:7709/151‑1 (MQ=60)

       ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1228:15755:4715/151‑1 (MQ=60)

       ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1166:20868:2329/151‑1 (MQ=60)

        CCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCC                                                                                                                                               <  LH00941:114:22FVMWLT1:2:2212:4448:5564/151‑1 (MQ=60)

         CATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCG                                                                                                                                              >  LH00941:114:22FVMWLT1:2:2228:9441:27085/1‑151 (MQ=60)

         CATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCA                                                                                                                                              >  LH00941:114:22FVMWLT1:2:1266:8072:7741/1‑151 (MQ=60)

                 CCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACGAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCTGAAGCCTATGTGGGAGCGGGAATGCCCACGAACGGG                                                                                                                                      >  LH00941:114:22FVMWLT1:2:2216:14868:22777/1‑151 (MQ=60)

                 CCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAACAGG                                                                                                                                      >  LH00941:114:22FVMWLT1:2:1155:29881:21400/1‑151 (MQ=60)

                   CACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAACGagac                                                                                                                                    >  LH00941:114:22FVMWLT1:2:2163:26590:20311/1‑147 (MQ=60)

                   CACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAGAGGCC                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1245:31832:12161/151‑1 (MQ=60)

                   CACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAGAGGCC                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1146:9191:9455/151‑1 (MQ=60)

                      AAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtcccacataggc                                                                                                                                 <  LH00941:114:22FVMWLT1:2:2164:3163:7181/151‑13 (MQ=60)

                      AAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtcccacataggc                                                                                                                                 <  LH00941:114:22FVMWLT1:2:2156:8035:27982/151‑13 (MQ=60)

                      AAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtcccacataggc                                                                                                                                 <  LH00941:114:22FVMWLT1:2:1224:41678:13234/151‑13 (MQ=60)

                       AGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCTGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGC                                                                                                                                <  LH00941:114:22FVMWLT1:2:2231:50572:4619/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAATTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2115:12177:5596/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTTGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2256:23206:26685/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGTCCCCGAACAGGCCTGGCC                                                                                                                               >  LH00941:114:22FVMWLT1:2:1150:4504:6300/1‑151 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1217:12223:27165/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2117:47928:4779/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2140:20119:2922/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2255:29539:11520/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2252:8063:23418/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1263:41188:18182/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1134:21302:20888/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2252:27200:13266/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1250:36575:20023/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1247:43693:7501/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2160:10716:20231/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2252:27182:13266/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2245:34689:18262/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2207:12223:3466/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2233:51949:21000/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2230:48972:5179/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1208:42399:16116/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2220:12898:26829/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2216:27570:27549/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1216:25083:20584/151‑1 (MQ=60)

                        GGCACGCCTGATGCAGAAGTTGAAAGGGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2102:3265:9086/151‑1 (MQ=60)

                             GCCTGATGCAGGAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGGGCACAAGCTGATCTGAGTGCGACATACGGGGGCCCGAAACTGGCCGAAGCCTATGTGTGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCGG                                                                                                                          <  LH00941:114:22FVMWLT1:2:1155:37601:24939/151‑1 (MQ=60)

                             GCCTGATGCAGAAGTTGAAAGTGGACTCGTTGGCGGAGCGGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:1101:43582:6060/151‑1 (MQ=60)

                             GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:2210:13185:28094/151‑1 (MQ=60)

                             GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:1126:27755:28414/151‑1 (MQ=60)

                             GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:1155:37592:24923/151‑1 (MQ=60)

                             GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:1212:40504:23338/151‑1 (MQ=60)

                             GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:1257:37952:19623/151‑1 (MQ=60)

                             GCCTGATGCAGAAGTTGAAAGTGAACTCGCGGGAGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:2132:38451:12577/151‑1 (MQ=60)

                             GCATGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCAAGGGAGCACAAGCTGATCGGATTGCGACAGACGGGTGCCCGAAACTTGCCGAAGCTTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:1112:14858:27757/151‑1 (MQ=60)

                                TGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATC                                                                                                                       <  LH00941:114:22FVMWLT1:2:2107:14562:22569/151‑1 (MQ=60)

                                TGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATC                                                                                                                       <  LH00941:114:22FVMWLT1:2:2131:36982:8238/151‑1 (MQ=60)

                                  ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATTCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                                     <  LH00941:114:22FVMWLT1:2:2222:24260:17557/151‑1 (MQ=60)

                                     acagAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:1223:7046:23546/147‑1 (MQ=60)

                                     CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:2109:15644:1705/151‑1 (MQ=60)

                                     CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:2128:12501:2281/151‑1 (MQ=60)

                                       GAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGG                                                                                                                <  LH00941:114:22FVMWLT1:2:2140:21515:25932/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1208:10337:25660/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1222:44387:23370/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1208:10347:25676/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2228:20294:7549/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1164:51774:14643/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2220:13324:6396/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2235:14572:16789/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2217:16532:4395/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2250:19897:25275/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2260:9496:4827/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2208:28365:17557/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2208:28356:17573/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2157:49046:18566/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2122:11715:15780/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2103:45700:19143/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2137:16763:7998/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1267:7592:23786/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1266:27515:28094/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1247:18223:25548/151‑1 (MQ=60)

                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1236:29022:2938/151‑1 (MQ=60)

                                          GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCTTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                                             <  LH00941:114:22FVMWLT1:2:2253:5438:12305/151‑1 (MQ=60)

                                          GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                                             <  LH00941:114:22FVMWLT1:2:2253:5419:12305/151‑1 (MQ=60)

                                          GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCGGATCTGATTGCGACATACGGGTGCCCGAAACTGGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                                             <  LH00941:114:22FVMWLT1:2:2250:49647:16917/151‑1 (MQ=60)

                                          GTTGAAAGTGAACTAGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATGGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                                             <  LH00941:114:22FVMWLT1:2:2238:42603:6060/151‑1 (MQ=60)

                                              AAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCC                                                                                                         <  LH00941:114:22FVMWLT1:2:2108:44646:14275/151‑1 (MQ=60)

                                              AAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCC                                                                                                         <  LH00941:114:22FVMWLT1:2:1103:50969:4763/151‑1 (MQ=60)

                                                    AACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTC                                                                                                   <  LH00941:114:22FVMWLT1:2:2165:40051:3242/151‑1 (MQ=60)

                                                        CGCTGGCGGAGTTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATATGGGTGCCCGAAACTTGCCGAAGCCTGTGTGGGAGCGGGCATGCCCGCGAAgaggccggctcagccatagagatcagcaggccaggcccgttcg                                                                                               <  LH00941:114:22FVMWLT1:2:1239:25259:11665/151‑44 (MQ=60)

                                                        CGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATATGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAgaggccggctcagccatagagatcagcaggccaggcccgttcg                                                                                               <  LH00941:114:22FVMWLT1:2:2127:26276:17429/151‑44 (MQ=60)

                                                          CTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCG                                                                                             <  LH00941:114:22FVMWLT1:2:2116:31231:11120/151‑1 (MQ=60)

                                                            GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                                           <  LH00941:114:22FVMWLT1:2:2162:41364:24827/151‑1 (MQ=60)

                                                            GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                                           <  LH00941:114:22FVMWLT1:2:1232:31407:11136/151‑1 (MQ=60)

                                                            GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATGGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGGGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                                           <  LH00941:114:22FVMWLT1:2:2255:47364:9631/151‑1 (MQ=60)

                                                              CGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCA                                                                                         <  LH00941:114:22FVMWLT1:2:1154:18602:25756/151‑1 (MQ=60)

                                                              CGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCA                                                                                         <  LH00941:114:22FVMWLT1:2:1151:17345:3178/151‑1 (MQ=60)

                                                              CGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCA                                                                                         <  LH00941:114:22FVMWLT1:2:1151:17336:3162/151‑1 (MQ=60)

                                                                  GCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCC                                                                                     <  LH00941:114:22FVMWLT1:2:2162:40569:16917/151‑1 (MQ=60)

                                                                  GCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCC                                                                                     <  LH00941:114:22FVMWLT1:2:1236:40578:13122/151‑1 (MQ=60)

                                                                   CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                                    <  LH00941:114:22FVMWLT1:2:2251:25462:11024/151‑1 (MQ=60)

                                                                   CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                                    <  LH00941:114:22FVMWLT1:2:2251:25472:11008/151‑1 (MQ=60)

                                                                   CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                                    <  LH00941:114:22FVMWLT1:2:2251:25481:11024/151‑1 (MQ=60)

                                                                   CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                                    <  LH00941:114:22FVMWLT1:2:2235:48880:19271/151‑1 (MQ=60)

                                                                     GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGC                                                                                  <  LH00941:114:22FVMWLT1:2:1255:6371:13346/151‑1 (MQ=60)

                                                                         AAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATACCCGCgaag                                                                              <  LH00941:114:22FVMWLT1:2:2205:40541:9983/151‑5 (MQ=60)

                                                                             ACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGGCGCCCGCGAACGGGCCcggcttgtttagttcccttggtgaaccggcccctcccccagcaggccaactcccccca                                                                          >  LH00941:114:22FVMWLT1:2:1156:36159:8542/1‑93 (MQ=60)

                                                                                             GCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCGCGAACaagaatggaacgctgagctctctggctgggccagcacccccgcgggtattcccaccccccccagtttcgttgttggctc                                                          >  LH00941:114:22FVMWLT1:2:2260:16199:4683/1‑72 (MQ=60)

                                                                                                            ACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCctgtctcttatacacatctccgagcccacgagacaagatacacgatctggggggggggggtttttttt                                           >  LH00941:114:22FVMWLT1:2:2111:24538:6188/1‑83 (MQ=60)

                                                                                                                        CGGAAACTTGCCGAAGCTGATGGGGGAGAGGGCGTGCCCGAGAATGGGCCTGGCCTGCTGATCTCTGTGGTTGATCTGGCCTCTTTGTGGGCATGCCCGCTCCCAAAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCT                               <  LH00941:114:22FVMWLT1:2:2266:47650:25948/151‑1 (MQ=60)

                                                                                                                                                     GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  LH00941:114:22FVMWLT1:2:1103:41207:2105/1‑151 (MQ=60)

GAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  AE015451_phaM1/499054‑499353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`