Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A6 F11 I4 R1 73 908.3 39027734 99.3% 38754539 136.2

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA AE015451_phaM1 499,203 A→G intergenic (+47/‑249) uhpA CDS (DNA‑binding response regulator UhpA) → / → PP_0411 CDS (Polyamine ABC transporter_ ATP‑binding protein) –/–

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AE015451_phaM1499,2030AG99.7% 1212.3 / ‑5.4 305intergenic (+47/‑249)uhpA CDS (DNA‑binding response regulator UhpA)/PP_0411 CDS (Polyamine ABC transporter_ ATP‑binding protein)–/–
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base G (72/232);  minor base T (0/1);  total (72/233)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 305 total aligned reads displayed.

CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCAGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATG‑CCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGG  >  AE015451_phaM1/499072‑499330
                                                                                                                                    |                                                                                                                                  
ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCAt                                                                                                                               <  1:3601750/137‑1 (MQ=255)
ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCAt                                                                                                                               <  1:18362744/137‑1 (MQ=255)
ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCAt                                                                                                                               <  1:14459495/137‑1 (MQ=255)
 cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATg                                                                                                                              <  2:15454900/137‑1 (MQ=255)
 cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATg                                                                                                                              <  2:16399785/137‑1 (MQ=255)
  acaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGc                                                                                                                             >  2:17309368/1‑137 (MQ=255)
   caATGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCCGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGGAGCGGGCATGc                                                                                                                             <  2:14837556/137‑1 (MQ=255)
    aaGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCTAAGCCTATGT‑GGGAGCGGGCATGccc                                                                                                                           <  2:3167296/137‑1 (MQ=255)
     aGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGGATGtcct                                                                                                                          >  1:3189925/1‑136 (MQ=255)
     aGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGGACGccca                                                                                                                          >  2:3996104/1‑136 (MQ=255)
      gtcACGCCTGATGCGGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCGGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCcgc                                                                                                                         <  1:17145316/135‑1 (MQ=255)
      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGTGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCcgc                                                                                                                         <  1:6314005/137‑1 (MQ=255)
      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCcgc                                                                                                                         <  1:13495633/137‑1 (MQ=255)
      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCcgc                                                                                                                         <  2:12008983/137‑1 (MQ=255)
      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCcgc                                                                                                                         <  1:4823461/137‑1 (MQ=255)
      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCcgc                                                                                                                         <  2:16112477/137‑1 (MQ=255)
      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCcgc                                                                                                                         <  1:14488219/137‑1 (MQ=255)
      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCcgc                                                                                                                         <  2:8061452/137‑1 (MQ=255)
      ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCcgc                                                                                                                         <  2:10173277/137‑1 (MQ=255)
      gccACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCcgc                                                                                                                         <  2:4615090/135‑1 (MQ=255)
           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                   <  1:14852795/137‑1 (MQ=255)
           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                   <  2:14616630/137‑1 (MQ=255)
           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                   <  2:12069265/137‑1 (MQ=255)
           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                   <  2:1165002/137‑1 (MQ=255)
           gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGAGTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATTT‑GGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                   <  1:13228242/137‑1 (MQ=255)
             cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGGATGACCGCaaaaagg                                                                                                                  >  2:6319624/1‑133 (MQ=255)
              tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGGATGACCACGCAcaagg                                                                                                                 >  2:11343016/1‑133 (MQ=255)
              tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGGAGGTCCGCGGAC‑g‑gga                                                                                                               >  1:17843609/1‑136 (MQ=255)
                aTGCATAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑ggcct                                                                                                             <  1:3246536/137‑1 (MQ=255)
                aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑ggcct                                                                                                             <  1:13597096/137‑1 (MQ=255)
                aTGCAGAAGTCGAAATTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGACTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑ggcct                                                                                                             <  2:5128198/137‑1 (MQ=255)
                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGTTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑ggcctggc                                                                                                          <  2:4540001/137‑1 (MQ=255)
                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGGATTGCCGAGAAC‑A‑ggactggg                                                                                                          >  1:7511982/1‑136 (MQ=255)
                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGGATGCCCGCGAAC‑A‑ggactggg                                                                                                          >  2:7206717/1‑136 (MQ=255)
                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGGAGGGCCGCGAAA‑g‑gggcgtga                                                                                                          >  1:10021581/1‑131 (MQ=255)
                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGGACGCCCGCGAAC‑A‑agactggc                                                                                                          >  2:5625500/1‑137 (MQ=255)
                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑ggcctggg                                                                                                          >  2:1849541/1‑136 (MQ=255)
                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑ggcctggc                                                                                                          <  2:3499484/137‑1 (MQ=255)
                   cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑A‑ggcccggc                                                                                                          >  2:16907907/1‑137 (MQ=255)
                   cAGAAGTTGAAAGTGAACCCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCGTGCCCGCGAAC‑G‑ggcctggc                                                                                                          <  1:10073606/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTTAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTTCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  2:9533167/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGTCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  1:1040712/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  1:16277501/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  2:15116677/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  1:2170961/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  2:15680986/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  2:1399964/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  1:19370572/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  2:13388213/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  2:18006166/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  2:1808895/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  2:9267981/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  1:13712970/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  2:429882/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  2:10614223/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  2:4418074/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  1:10887930/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  1:9394206/137‑1 (MQ=255)
                      aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCAAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  2:18814143/137‑1 (MQ=255)
                      aaGGTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGTTGATCTGATTGCGGCATACGGGTGCCTGAAACTTGTCGAAGCCTATGT‑GGGAGCTGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  2:12279911/137‑1 (MQ=255)
                       aGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCctgc                                                                                                      <  2:16252445/137‑1 (MQ=255)
                       aGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCctgc                                                                                                      <  2:17725782/137‑1 (MQ=255)
                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCctgct                                                                                                     <  2:10274523/137‑1 (MQ=255)
                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCctgct                                                                                                     <  1:8944230/137‑1 (MQ=255)
                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCctgct                                                                                                     <  1:18152337/137‑1 (MQ=255)
                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCctgct                                                                                                     <  2:8505483/137‑1 (MQ=255)
                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCctgct                                                                                                     <  1:16016816/137‑1 (MQ=255)
                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCctgct                                                                                                     <  2:18305900/137‑1 (MQ=255)
                        gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCctgct                                                                                                     <  1:715398/137‑1 (MQ=255)
                          tGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCACGCCCACGAAC‑A‑GGACTTGCccgctgg                                                                                                   >  2:13618762/1‑136 (MQ=255)
                           gAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGAt                                                                                                  <  1:18689894/137‑1 (MQ=255)
                            aaaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGAtc                                                                                                 <  1:157445/137‑1 (MQ=255)
                              aGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGGATGACCGCAAAA‑A‑GGACTTGGCTGCTGAtccc                                                                                               >  1:2511405/1‑135 (MQ=255)
                                tGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTa                                                                                             <  1:115944/137‑1 (MQ=255)
                                          ggCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTATGGCTGAGcc                                                                                   <  1:5800125/137‑1 (MQ=255)
                                          ggCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTATGGCTGAGcc                                                                                   <  1:394429/137‑1 (MQ=255)
                                          ggCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTATGGCTGAGcc                                                                                   <  2:4883799/137‑1 (MQ=255)
                                          ggCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTATGGCTGAGcc                                                                                   <  2:13857390/137‑1 (MQ=255)
                                          ggCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACNTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTATGGCTGAGcc                                                                                   <  1:16811582/137‑1 (MQ=255)
                                          ggCGGAGCTGATGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTATGGCTGAGcc                                                                                   <  2:2736472/137‑1 (MQ=255)
                                            cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATATGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTATGGCTGAGCCgg                                                                                 <  1:9948480/137‑1 (MQ=255)
                                            cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTATGGCTGAGCCgg                                                                                 <  2:1899295/137‑1 (MQ=255)
                                            cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGAACGCCCCCGAAC‑G‑GGTCTGGCCTCTTGGTCTCCCTGGCTGAggcag                                                                                 >  2:9745374/1‑133 (MQ=255)
                                                 cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctct                                                                            <  1:10821003/137‑1 (MQ=255)
                                                 cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctct                                                                            <  1:3534044/137‑1 (MQ=255)
                                                 cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctct                                                                            <  1:8747258/137‑1 (MQ=255)
                                                         gTACACCAGGGAGCAAAAGGGGATCTGATTGCGACAGACGGGTGCCCGAAACTTACCGAAGCTTATGT‑GGGAGTGGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGc                                                                    <  1:12581013/137‑1 (MQ=255)
                                                                 tGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGGATGGCCGGAAAA‑A‑GGACTTGCCTTTTTGCCTCTCTTGCTGAGCCTGCCCCCTCTCGGGGATG‑cccac                                                           >  1:9747823/1‑135 (MQ=255)
                                                                 tGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGGAGGCCCCCAAACAG‑GAACGGGCCT‑CTGGTCTCTCTGGGTGAGCCAGACCCCTCTCGGGAAGG‑cccca                                                           >  1:12004855/1‑135 (MQ=255)
                                                                    aGCACAAGCTGATCTGATTGCGACATACAGGTGCCCGAAACTTGCCGAAACCTATGT‑GGGAGCGGGGACACCAGCCAAC‑G‑GAACCCGCCTGCTCATCTCTCTGGCTTAGCCAGCCTCTTCGCGAGCATT‑CCCGCTcc                                                        >  2:5435648/1‑137 (MQ=255)
                                                                         aaGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       <  2:988195/86‑1 (MQ=255)
                                                                         aaGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       >  1:988195/1‑86 (MQ=255)
                                                                                 ctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGAATACCCACGAAC‑A‑GGCACGGCCTGCCGATCTCTATGGCTGAGCCGGCCCCCTCTCGAGCATG‑CCCCCCCCCACACGTTTcttt                                           >  2:3743839/1‑134 (MQ=255)
                                                                                        ggACGTACGGGTGCCCGAAGCTTGCTGAAGCTTGTGT‑GGGAGAGGGCGTGCCGGCGAGT‑G‑GCCCTGTCTTGTTGTTCTCTGTGGTTGGTCTGTCTTTTTTGTGGGCGTG‑CCCGCTCCCACAGGTTTCGTGGTTGGCt                                    <  2:8772664/136‑1 (MQ=255)
                                                                                         gACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCACGCCCACGAAA‑A‑GGACCGGCCTGCTGATCTCTCTGGTTTAGCCGGCCCCTTCTCGGGGATGTCCCCCCCCCACA‑GTTTCGTTGTTTGCTc                                   >  2:6156032/1‑137 (MQ=255)
                                                                                             tatgggTGCCCAAAACTTACCGAAGCTGATGT‑GGGAGCGGGCAGTCCTGAAAAT‑G‑GGCTTGTCCGGCTGCTTTCGGTGGCGGAGCTGGCCTCTTCGCGGGCATC‑CCCGCTCCCACAGGTTTCGTGGTTGGCTCGCaa                               <  2:2126557/134‑1 (MQ=255)
                                                                                                 ggTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCACGAAC‑GAGGCCGGCCCTGTTGGT‑TTTCCGGATGAGCCGGCCTCTTCTCGGGCACG‑CCCCCTCCCACAGGGTTCGTTGTTTGTTCGCCAgggg                           >  2:10989258/1‑136 (MQ=255)
                                                                                                        gAAACTTGCCGAAGCCTATGT‑GGGAGCGGGGATGCCCGCGAAC‑G‑GGCATTGACCGCTTATCTCTCTGGGTGCGCCGGGCTCTTTGTGGGTAGG‑CCCGCTTCCCCGGGTTTTTTGGGTGGGTCGCAAGGGGATTTCaa                    >  2:12893626/1‑137 (MQ=255)
                                                                                                        gAAACTTGCCGAAGCCTATGT‑GGGAGCGGGCATGCCCGCGAAC‑G‑Ggcctggcctg                                                                                                       >  2:10877903/1‑55 (MQ=255)
                                                                                                             ttGCAGAAGCCTATGT‑GGGAGTGGACGGTCCCGGGAGT‑G‑GTCTTGGTCGGCGGATCTCTGTGGCGGGGCCGTCTTTTTTTGGGGCATC‑CCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCg               <  2:11933797/137‑1 (MQ=255)
                                                                                                                                    |                                                                                                                                  
CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGT‑GGGAGCAGGCATGCCCGCGAAC‑G‑GGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATG‑CCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGG  >  AE015451_phaM1/499072‑499330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCT  >  AE015451_phaM1/499054‑499339
                                                                                                                                                     |                                                                                                                                        
GAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGG                                                                                                                                         >  LH00941:114:22FVMWLT1:2:1126:20895:12721/1‑151 (MQ=60)
GAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGG                                                                                                                                         >  LH00941:114:22FVMWLT1:2:2236:38128:6508/1‑151 (MQ=60)
 AGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATNGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG                                                                                                                                        >  LH00941:114:22FVMWLT1:2:2232:20479:19943/1‑151 (MQ=60)
 AGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG                                                                                                                                        >  LH00941:114:22FVMWLT1:2:2269:45274:22729/1‑151 (MQ=60)
 AGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG                                                                                                                                        >  LH00941:114:22FVMWLT1:2:1117:30935:26332/1‑151 (MQ=60)
 AGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGG                                                                                                                                        <  LH00941:114:22FVMWLT1:2:1116:44553:24395/151‑1 (MQ=60)
       ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                  <  LH00941:114:22FVMWLT1:2:2132:28310:14355/151‑1 (MQ=60)
       ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                  <  LH00941:114:22FVMWLT1:2:2132:28328:14355/151‑1 (MQ=60)
       ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                  <  LH00941:114:22FVMWLT1:2:1158:35280:5772/151‑1 (MQ=60)
       ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                  <  LH00941:114:22FVMWLT1:2:2231:42750:13618/151‑1 (MQ=60)
       ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                  <  LH00941:114:22FVMWLT1:2:1201:11096:20984/151‑1 (MQ=60)
       ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                  <  LH00941:114:22FVMWLT1:2:1205:10790:11488/151‑1 (MQ=60)
       ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCC                                                                                                                                  >  LH00941:114:22FVMWLT1:2:1162:44840:20984/1‑151 (MQ=60)
         CATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCG                                                                                                                                <  LH00941:114:22FVMWLT1:2:1164:15145:23482/151‑1 (MQ=60)
           TCAGCACCCATAAGGCACGCCTGATGCAGAGGGTGAAAGTGAACTCGCTTGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGAGCTGATTGCGACNTACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG                                                                                                                              <  LH00941:114:22FVMWLT1:2:2261:8821:3242/151‑1 (MQ=60)
           TCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACNTACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG                                                                                                                              <  LH00941:114:22FVMWLT1:2:2261:8812:3258/151‑1 (MQ=60)
           TCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCG                                                                                                                              <  LH00941:114:22FVMWLT1:2:2116:25980:8462/151‑1 (MQ=60)
            CAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGA                                                                                                                             <  LH00941:114:22FVMWLT1:2:1121:34680:2073/151‑1 (MQ=60)
            CAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGA                                                                                                                             <  LH00941:114:22FVMWLT1:2:1121:34670:2057/151‑1 (MQ=60)
                 CCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGG                                                                                                                        >  LH00941:114:22FVMWLT1:2:2241:23022:4587/1‑151 (MQ=60)
                 CCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGG                                                                                                                        >  LH00941:114:22FVMWLT1:2:2169:16578:5083/1‑151 (MQ=60)
                  CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGatcccacat                                                                                                                       <  LH00941:114:22FVMWLT1:2:1157:9404:25772/151‑10 (MQ=60)
                  CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtcccacat                                                                                                                       <  LH00941:114:22FVMWLT1:2:2251:32026:27165/151‑9 (MQ=60)
                  CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGC                                                                                                                       <  LH00941:114:22FVMWLT1:2:1152:49398:27181/151‑1 (MQ=60)
                  CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGC                                                                                                                       <  LH00941:114:22FVMWLT1:2:2254:25795:22809/151‑1 (MQ=60)
                  CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGC                                                                                                                       <  LH00941:114:22FVMWLT1:2:1152:51533:27998/151‑1 (MQ=60)
                  CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGC                                                                                                                       <  LH00941:114:22FVMWLT1:2:2168:51542:26476/151‑1 (MQ=60)
                      AAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtcccacataggc                                                                                                                   <  LH00941:114:22FVMWLT1:2:1157:36371:23386/151‑13 (MQ=60)
                        gggctcggagatgtgtataagagacagGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2215:36251:5852/124‑1 (MQ=60)
                        GTCACGCCTGATGCGGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCGGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2237:2257:16725/151‑1 (MQ=60)
                        GTCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2237:2248:16709/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAATTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2160:9589:20520/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAATTCGCTGGCGGAGCTGGTGAAGTACACCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2102:47003:23322/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTTGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGGGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2154:2803:14787/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGTGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:1221:19712:28798/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCGTGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGTGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:1222:28569:28606/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2155:47946:5227/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2158:24880:4219/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2103:16180:20119/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2169:24011:9663/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2169:24029:9663/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2223:21561:10223/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2233:19989:16500/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2247:51505:19783/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2257:33792:19975/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:1269:15931:13922/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2263:6149:25612/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:1247:26646:12817/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:2106:18186:15780/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:1217:43268:19831/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:1105:23271:23786/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:1120:45468:23738/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:1115:42750:16693/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:1157:46790:26252/151‑1 (MQ=60)
                        GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                 <  LH00941:114:22FVMWLT1:2:1170:44812:14082/151‑1 (MQ=60)
                             GCCTGATGCAGAAGTTGAAAGTGAATTCGCGGGCGGAGCTGGTGAAGTATGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAATCCTATTTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                            <  LH00941:114:22FVMWLT1:2:2210:2396:12898/151‑1 (MQ=60)
                             GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAGGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                            <  LH00941:114:22FVMWLT1:2:2230:16402:17910/151‑1 (MQ=60)
                             GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                            <  LH00941:114:22FVMWLT1:2:1102:22402:14979/151‑1 (MQ=60)
                             GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                            <  LH00941:114:22FVMWLT1:2:2204:5632:18502/151‑1 (MQ=60)
                             GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                            <  LH00941:114:22FVMWLT1:2:2159:36972:16741/151‑1 (MQ=60)
                             GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGAGTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATTTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                            <  LH00941:114:22FVMWLT1:2:2151:2202:9263/151‑1 (MQ=60)
                             GCCTGATGCAGAAGTTGAAAGGAAACTAGCTGGCGGAGCTGGGGAAGTACGCCAGGGAGCACAAGCGGATCTGGGGGAGAAGTACGGGTGCCCGAAACTGGCCGAAGCCTATGTGGGGGTGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                            <  LH00941:114:22FVMWLT1:2:1132:15598:19335/151‑1 (MQ=60)
                                TGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATC                                                                                                         <  LH00941:114:22FVMWLT1:2:2269:15644:16981/151‑1 (MQ=60)
                                  ATGCATAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                       <  LH00941:114:22FVMWLT1:2:1147:50720:25468/151‑1 (MQ=60)
                                  ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                       <  LH00941:114:22FVMWLT1:2:2156:39450:18214/151‑1 (MQ=60)
                                  ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                       <  LH00941:114:22FVMWLT1:2:2156:39431:18214/151‑1 (MQ=60)
                                  ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                       <  LH00941:114:22FVMWLT1:2:2169:38137:23690/151‑1 (MQ=60)
                                  ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                       <  LH00941:114:22FVMWLT1:2:1101:38063:6620/151‑1 (MQ=60)
                                  ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                       <  LH00941:114:22FVMWLT1:2:2107:46643:4635/151‑1 (MQ=60)
                                  ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                       <  LH00941:114:22FVMWLT1:2:1147:50710:25452/151‑1 (MQ=60)
                                  ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                       <  LH00941:114:22FVMWLT1:2:2141:13416:11296/151‑1 (MQ=60)
                                   TGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCT                                                                                                      <  LH00941:114:22FVMWLT1:2:2142:39579:15844/151‑1 (MQ=60)
                                     CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                    <  LH00941:114:22FVMWLT1:2:2105:14433:12865/151‑1 (MQ=60)
                                     CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                    <  LH00941:114:22FVMWLT1:2:1124:47780:5484/151‑1 (MQ=60)
                                     CAGAAGTTGAAAGTGAACCCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCGTGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                    <  LH00941:114:22FVMWLT1:2:2105:14424:12882/151‑1 (MQ=60)
                                      AGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATG                                                                                                   <  LH00941:114:22FVMWLT1:2:1203:9524:14387/151‑1 (MQ=60)
                                       GAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGG                                                                                                  <  LH00941:114:22FVMWLT1:2:2253:2257:2601/151‑1 (MQ=60)
                                        aaagtggaagtgaacctgtcgggtgaattgggGAAGTACGCCATGGAGCACAGGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCGATGGGGGAGCGGGCAGGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1138:42223:27853/119‑1 (MQ=60)
                                        aaaacaaaccccccccccccagattggcaatattgtctcgtgggctcggagatgtgtataagagacaGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:2117:32692:8526/84‑1 (MQ=60)
                                        AGGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1270:15256:18486/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGTTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCGGATTGCGACAGAAGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1150:45099:16885/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGTCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1116:25712:7389/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1242:32359:18550/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:2225:30917:5580/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:2268:37342:28910/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1258:42686:1304/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1258:42676:1320/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1137:50304:17029/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:2158:24066:8638/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1136:13000:6284/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1132:19074:14755/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1235:30501:19847/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:2152:24418:6909/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1269:38304:5564/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:2137:39062:27245/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1118:25860:10047/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:2117:3820:12737/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1265:36741:17781/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:2241:33459:9343/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1268:48020:27837/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1270:43231:20087/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGACATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:1131:17576:21961/151‑1 (MQ=60)
                                        AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGAGTGCGACAGACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                 <  LH00941:114:22FVMWLT1:2:2117:3838:12737/151‑1 (MQ=60)
                                          GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                               <  LH00941:114:22FVMWLT1:2:2251:20932:23226/151‑1 (MQ=60)
                                          GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                               <  LH00941:114:22FVMWLT1:2:1111:3727:14819/151‑1 (MQ=60)
                                          GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                               <  LH00941:114:22FVMWLT1:2:2221:24122:13154/151‑1 (MQ=60)
                                          GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                               <  LH00941:114:22FVMWLT1:2:2220:26350:28638/151‑1 (MQ=60)
                                          GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                               <  LH00941:114:22FVMWLT1:2:2158:25740:1576/151‑1 (MQ=60)
                                          GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                               <  LH00941:114:22FVMWLT1:2:1141:10522:4811/151‑1 (MQ=60)
                                          GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                               <  LH00941:114:22FVMWLT1:2:1259:15737:7277/151‑1 (MQ=60)
                                          GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                               <  LH00941:114:22FVMWLT1:2:2114:48186:18486/151‑1 (MQ=60)
                                             GAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGC                                                                                            <  LH00941:114:22FVMWLT1:2:2259:30205:11552/151‑1 (MQ=60)
                                              AAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCC                                                                                           <  LH00941:114:22FVMWLT1:2:1103:9579:10416/151‑1 (MQ=60)
                                                  TGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCC                                                                                       <  LH00941:114:22FVMWLT1:2:1102:35465:21432/151‑1 (MQ=60)
                                                            GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACGTACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                             <  LH00941:114:22FVMWLT1:2:2113:15515:2249/151‑1 (MQ=60)
                                                            GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGTCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCctgtctcttatacacatctccgagcccacgagacaa                                                                             >  LH00941:114:22FVMWLT1:2:1127:35280:28414/1‑115 (MQ=60)
                                                            GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                             <  LH00941:114:22FVMWLT1:2:1214:40643:18582/151‑1 (MQ=60)
                                                            GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                             <  LH00941:114:22FVMWLT1:2:1227:34199:13154/151‑1 (MQ=60)
                                                            GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                             <  LH00941:114:22FVMWLT1:2:1214:40624:18582/151‑1 (MQ=60)
                                                            GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                             <  LH00941:114:22FVMWLT1:2:1106:43435:23834/151‑1 (MQ=60)
                                                            GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                             <  LH00941:114:22FVMWLT1:2:1106:43453:23834/151‑1 (MQ=60)
                                                            GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                             <  LH00941:114:22FVMWLT1:2:1147:16291:9871/151‑1 (MQ=60)
                                                            GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACNTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                             <  LH00941:114:22FVMWLT1:2:2232:51071:23674/151‑1 (MQ=60)
                                                              CGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATATGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCA                                                                           <  LH00941:114:22FVMWLT1:2:2103:2960:16500/151‑1 (MQ=60)
                                                                  GCTGGTGAGGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCC                                                                       <  LH00941:114:22FVMWLT1:2:2231:10707:6188/151‑1 (MQ=60)
                                                                   CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                      <  LH00941:114:22FVMWLT1:2:2116:33200:13538/151‑1 (MQ=60)
                                                                   CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                      <  LH00941:114:22FVMWLT1:2:1151:11817:29022/151‑1 (MQ=60)
                                                                   CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                      <  LH00941:114:22FVMWLT1:2:1256:19814:13858/151‑1 (MQ=60)
                                                                   CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGAGTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                      <  LH00941:114:22FVMWLT1:2:1151:11807:29038/151‑1 (MQ=60)
                                                                   CGGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                      <  LH00941:114:22FVMWLT1:2:2169:41807:2409/151‑1 (MQ=60)
                                                                     GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCGCGctgtctcttatacacatctccgagcccacgagacaatattgccaatctgggggggggg                                                                    >  LH00941:114:22FVMWLT1:2:2212:24085:2906/1‑93 (MQ=60)
                                                                          AGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAAaagggctggaatgtgggtttttcttgttgggctggcctcttctccggaacgccccgtcccc                                                               >  LH00941:114:22FVMWLT1:2:1116:23271:5596/1‑90 (MQ=60)
                                                                           GTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCTGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCgaagag                                                              <  LH00941:114:22FVMWLT1:2:2225:21117:2217/151‑7 (MQ=60)
                                                                           GTACACCAGGGAGCAAAAGGGGATCTGATTGCGACAGACGGGTGCCCGAAACTTACCGAAGCTTATGTGGGAGTGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCAGGCCCGCgaagag                                                              <  LH00941:114:22FVMWLT1:2:2141:11844:29359/151‑7 (MQ=60)
                                                                             ACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAAagggaacgggatgctgagctccctggttggagcggactcttcaggagaaggaccactcccccaa                                                            >  LH00941:114:22FVMWLT1:2:2263:3718:29247/1‑87 (MQ=60)
                                                                             ACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCctgtctcttatacacatctccgagcccacgagacaat                                                            >  LH00941:114:22FVMWLT1:2:1115:26683:14291/1‑114 (MQ=60)
                                                                             ACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCGCcaatgggctttgtttttttatttcttttgctgagtctgtctcttcgctggtattcccttctcccccg                                                            >  LH00941:114:22FVMWLT1:2:2151:50525:21416/1‑84 (MQ=60)
                                                                                                            ACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCACGAACaagaccggccctacgatccctccgacccagacagccactccttagggacaaccaaccacacaaagttcgcggttgtaccatcaaagaaatccca                             >  LH00941:114:22FVMWLT1:2:2170:1555:27709/1‑57 (MQ=60)
                                                                                                                             ACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGACATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAG            <  LH00941:114:22FVMWLT1:2:2248:5706:27789/151‑1 (MQ=60)
                                                                                                                                       GCCTATTTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGGTCTCTCTGGTTGAGCCGGCCTCTTCGCGGGCACGCCCGCTCCCCCAAGTTTCGTGGTTGGCTCACAAGGGAATTCCCACCCCGCCATCCTTGTAGGGCGATCCCC  >  LH00941:114:22FVMWLT1:2:2159:33737:17349/1‑151 (MQ=60)
                                                                                                                                                     |                                                                                                                                        
GAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCT  >  AE015451_phaM1/499054‑499339

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 9 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑