Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F42 I4 R1	76	794.2	35354760	99.3%	35107276	136.2

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	499,203	A→G	intergenic (+47/‑249)	uhpA CDS (DNA‑binding response regulator UhpA) → / → PP_0411 CDS (Polyamine ABC transporter_ ATP‑binding protein)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	499,203	0	A	G	100.0%	725.9 / NA	188	intergenic (+47/‑249)	uhpA CDS (DNA‑binding response regulator UhpA)/PP_0411 CDS (Polyamine ABC transporter_ ATP‑binding protein)	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); new base G (31/157); total (31/157)

Only 100 of 189 total aligned reads displayed.

ACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCGG‑CCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGG  >  AE015451_phaM1/499070‑499330

  ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGGGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                               <  1:3854377/137‑1 (MQ=255)

   cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCggggacg                                                                                                                              >  2:2848559/1‑133 (MQ=255)

   cacaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATg                                                                                                                              <  1:8052421/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGGGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:2245492/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:8498533/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:16126907/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:14234609/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:17111622/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:16962360/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:9547063/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:17111634/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:7580618/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:14448942/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:1344119/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:4823061/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:1105593/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:4150175/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGACCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  2:336100/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGNGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                         <  1:17450582/137‑1 (MQ=255)

           aCGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGaa                                                                                                                      <  1:12807948/137‑1 (MQ=255)

             gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                   <  1:14752509/137‑1 (MQ=255)

             gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                   <  1:5465701/137‑1 (MQ=255)

             gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                   <  1:12281071/137‑1 (MQ=255)

             gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                   <  2:95772/137‑1 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATTCCCTCGaaacgg                                                                                                                  >  1:12232440/1‑133 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAAC‑agg                                                                                                                 >  2:15970625/1‑137 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCCaaaagg                                                                                                                  >  1:10808906/1‑133 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGAGGTCCGCGaaaagg                                                                                                                  >  1:1574647/1‑133 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGAGGCCcgcggaaaag                                                                                                                  >  1:13544754/1‑131 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGACGCACGCGAAcaag                                                                                                                  >  1:7133809/1‑134 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCACGAAC‑agg                                                                                                                 >  2:1883361/1‑137 (MQ=255)

                tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggc                                                                                                                <  2:11892847/137‑1 (MQ=255)

                tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggc                                                                                                                <  1:4749416/137‑1 (MQ=255)

                tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggc                                                                                                                <  2:12348595/137‑1 (MQ=255)

                tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggc                                                                                                                <  1:10260202/137‑1 (MQ=255)

                tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggc                                                                                                                <  1:15466429/137‑1 (MQ=255)

                tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggc                                                                                                                <  1:17216561/137‑1 (MQ=255)

                  aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcct                                                                                                              <  1:2741013/137‑1 (MQ=255)

                  aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcct                                                                                                              <  1:11871867/137‑1 (MQ=255)

                  aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcct                                                                                                              <  1:12374535/137‑1 (MQ=255)

                   tGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctg                                                                                                             <  2:811891/137‑1 (MQ=255)

                   tGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAACGCCCGCGAAC‑Gggactg                                                                                                             >  2:10565852/1‑137 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGGGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                           <  1:1730343/137‑1 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                           <  1:596380/137‑1 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                           <  2:592727/137‑1 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                           <  1:4476012/137‑1 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCACGAAA‑Aggcccgga                                                                                                           >  2:12556233/1‑136 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCACAAAC‑Ggggctggg                                                                                                           >  1:17293956/1‑136 (MQ=255)

                        aatttGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCAGGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:13924252/134‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGTTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctgacct‑g                                                                                                       <  1:15449510/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:7778738/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:10455945/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:6743325/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:5647668/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:5578782/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:5144807/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:4728459/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:16713617/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:6785124/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:15067040/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:4583168/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:8752510/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:11805484/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:23661/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATGGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:16907494/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCGGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  1:499052/137‑1 (MQ=255)

                        aaGTGGAAAGTAAACTTGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACACGCTGATCTGAGTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATTTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcct‑g                                                                                                       <  2:7769362/137‑1 (MQ=255)

                          gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCct‑gct                                                                                                     <  2:14107632/137‑1 (MQ=255)

                          gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCct‑gct                                                                                                     <  2:6662830/137‑1 (MQ=255)

                          gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCAAGAAC‑AGGACTGGCct‑gct                                                                                                     >  1:7789180/1‑137 (MQ=255)

                          gTTGAAAGTGAACCCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCct‑gct                                                                                                     <  1:3578909/137‑1 (MQ=255)

                            tGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGa                                                                                                   <  1:11264331/137‑1 (MQ=255)

                                  tGAACTCGCTGTAGGAGCGGGTGAAGGACGCCATGGAGCACAAGCGGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGTGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTa                                                                                             <  1:11417717/137‑1 (MQ=255)

                                  tGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTa                                                                                             <  2:7129498/137‑1 (MQ=255)

                                  tGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTa                                                                                             <  2:11551610/137‑1 (MQ=255)

                                            ggCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGCTGCCCGCTAAC‑AGGACTTGCCT‑GTTGGTCTCCCTGGATGAGcc                                                                                   >  2:4927698/1‑137 (MQ=255)

                                              cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTTGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCgg                                                                                 <  2:7503247/137‑1 (MQ=255)

                                              cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCgg                                                                                 <  1:12108631/137‑1 (MQ=255)

                                              cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCgg                                                                                 <  2:5526179/137‑1 (MQ=255)

                                                   cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCGG‑Cctct                                                                           <  1:4369543/137‑1 (MQ=255)

                                                   cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCGG‑Cctct                                                                           <  2:13599699/137‑1 (MQ=255)

                                                   cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCGG‑Cctct                                                                           <  1:231886/137‑1 (MQ=255)

                                                             aCGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCGG‑CCTCTTCGCGGGCAt                                                                 <  2:15561256/137‑1 (MQ=255)

                                                                      aGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAAC‑AAGACCGGCCT‑GCTGGTCTCTCCTGCTGAGCCGG‑CCTCTTCTCTGGCCTGtccccccc                                                        >  2:3663462/1‑132 (MQ=255)

                                                                      aGCACAAGCTGACCTGGTTGCGACATACGGGTTCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCGGGCCT‑GCTGATCTCTATGGCTGAGCCGG‑CCTCTTCGCGGGCATGCCCGcgaa                                                        <  2:5741736/137‑4 (MQ=255)

                                                                             gCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCACGAAC‑AGGACCCGCCC‑GCTGGTTTCTCTGGATGAGACAG‑CCCCCTCACTGGCACGCCCCCTccccaaagg                                                 >  2:12303462/1‑131 (MQ=255)

                                                                                gatctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCTAAC‑AGGCCTTGCCT‑TCTGGTTTCTCTGGCTTAGTCAG‑CCTCTTCTCGGGCACGCCCTCTCCCCCCAGTTTc                                              >  2:16979820/1‑137 (MQ=255)

                                                                                    tgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGAGGTCCACGAAC‑AGGCCTGGACC‑TCTGGTCTCTCTTGCTGGGCCGGACCCCTTCCCGGG‑ACGTCCCCTCCCCCAAGttttcttt                                          >  2:11862622/1‑132 (MQ=255)

                                                                                               tACGGGGACCCGAACTTTGCCGAATCCTTTGTGGGAGCGGACGTTCCCGAGAAT‑GGCCTTGGCTT‑GCTGATCTTTATGTTTGAGCTGT‑CTTTTTGGTGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCaa                               <  2:10110158/137‑1 (MQ=255)

                                                                                               ggtgggTGCTGGAAATTTTCCGAAGCTTGGTTGGGAGAGGGCGTGCCCGAGAAG‑GGGCCGGTCCT‑GAGGCTTTCCAGGGTGGCCCCGC‑CCTCTTCGGGGGCGTGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCaa                               <  2:16596083/134‑1 (MQ=255)

                                                                                               gactggTGCCCGACACTTGCTAAAATCCATGTGGAGGCGGGCATTCAAGAAAAC‑GGG‑CTGGCTTGGCTGATGCCTGTTACTAACCCTT‑CCTCTCCGAGGTCATGCCCGCGCCCACAGGTTTCGTGGTTGGCTCGCaa                               <  2:7814679/133‑1 (MQ=255)

                                                                                                             ttttGCCGAGGCTTATGTGGGAGCGGGCCTTCCCGCGAAC‑GGGCTTGTCTT‑GTTGCTCTCCATGGCTAATCCTC‑CTTTTTCGCGGGCATCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATcc                 <  2:14727719/135‑1 (MQ=255)

                                                                                                             atttGTCGAAGCCTAGTGGGGAGGGGGTGTGCCCGAGAAG‑GGGCTTGGCCT‑GTTGGTCCTGGTGTCTGAGCCGG‑CCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATcc                 <  2:16328086/135‑1 (MQ=19)

                                                                                                             atttGCGGAGGCTTGTGGGGGAGGGGGCATGCTCGAGAAG‑GGGTTTGGCCT‑GCGGATCTCTGTGTTGGATCCTT‑CTTCTTCGCGGGCGTTCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATcc                 <  2:4268867/135‑1 (MQ=255)

                                                                                                               tttctgAAGCCTGTGGGGGAGCGGGCGTGCCCG‑GAACGGGGCCGGGCCT‑GTGGGTCTCCATGTCTGAGCTGC‑CCTCTTCGTGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCg               <  2:17053229/132‑1 (MQ=19)

                                                                                                               ttGCCGAACCTTGGGGGGGAGGGGGCGTGCCCGAGAAT‑GGCCTTGTCTG‑GTTGATTTCGCTGGTTGGGCTGT‑CCTTTTCGTGGGCCTCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCg               <  2:10842989/137‑1 (MQ=255)

                                                                                                                          tgtgtgGGAGAGGGCAGCCCCGAAAAC‑GGGCCTGGCCT‑GCTGCTCTCTGTGGTGGACCTGC‑CCTCTTTTTGGGCCTTCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTa    <  2:1440197/135‑1 (MQ=255)

                                                                                                                            tgggggAGGGGGCGTGCCCGAGAAT‑GGGTCTGGTTT‑GCGGATTTTTGTGTCGAAGCTGC‑CTTCTTCGTGGCCCTCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAgg  <  1:14924886/134‑1 (MQ=255)

                                                                                                                            tgggggAGCGGGTATGCCCGAGGAT‑GTGCCTGTTTT‑GTTGATCTCGGTTGCTGAGCCGC‑CTTCTGTGCGGGCATCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAgg  <  1:3234471/134‑1 (MQ=255)

                                                                                                                            tgggggAGCGGCCGTCCCCGCAAAT‑GGCCCTTTCCT‑GCTGCTCTTTATGGCTGCTCCGT‑CTTCTTCGCGGACATCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAgg  <  1:16247726/134‑1 (MQ=255)

ACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAAC‑GGGCCTGGCCT‑GCTGATCTCTATGGCTGAGCCGG‑CCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGG  >  AE015451_phaM1/499070‑499330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  AE015451_phaM1/499057‑499353

CAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCA                                                                                                                                                    <  LH00941:114:22FVMWLT1:2:2231:4476:18294/151‑1 (MQ=60)

    ccaaaaaaaaaaacgacataccagatgtcttctaatgtctcgtgggctcggagatgtgtataagagacagACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1228:7046:23770/81‑1 (MQ=60)

    ACCATCAGCACCCACAAGGCACGCCTTATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2115:14341:17541/151‑1 (MQ=60)

    ACCATCAGCACCCACAAGGCACGCCTTATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2115:14359:17541/151‑1 (MQ=60)

    ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2165:45284:12369/151‑1 (MQ=60)

    ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1258:34892:13906/151‑1 (MQ=60)

    ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1213:1971:9983/151‑1 (MQ=60)

    ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1270:48233:24299/151‑1 (MQ=60)

    ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2101:42630:14787/151‑1 (MQ=60)

    ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1123:15810:25596/151‑1 (MQ=60)

    ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2257:24787:4795/151‑1 (MQ=60)

     CCATCAGCACCCACAAGGCACGCCTGATGCATAATTTGAAAGTGAACTCGCTGGCGGAACTGGTGAAGTACGCCATTGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACGTGCCGAAGCCTATGTGGGAGCGGGCATGCCC                                                                                                                                               <  LH00941:114:22FVMWLT1:2:2153:4837:14531/151‑1 (MQ=60)

       ATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGC                                                                                                                                             <  LH00941:114:22FVMWLT1:2:1118:21404:6396/151‑1 (MQ=60)

       ATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGC                                                                                                                                             <  LH00941:114:22FVMWLT1:2:1118:21413:6380/151‑1 (MQ=60)

         CAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtc                                                                                                                                           <  LH00941:114:22FVMWLT1:2:2164:25342:8862/151‑3 (MQ=60)

             ACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtcccac                                                                                                                                       <  LH00941:114:22FVMWLT1:2:1126:34735:25836/151‑7 (MQ=60)

              CCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCcaaaagg                                                                                                                                      >  LH00941:114:22FVMWLT1:2:2132:20794:26604/1‑144 (MQ=60)

               CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGGGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtcccacat                                                                                                                                     <  LH00941:114:22FVMWLT1:2:1162:11881:15171/151‑9 (MQ=60)

                CACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCC                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1258:35937:23594/151‑1 (MQ=60)

                    AGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAACGGGCCTGGC                                                                                                                                >  LH00941:114:22FVMWLT1:2:1118:25629:9647/1‑151 (MQ=60)

                     cccccccccccccccccccccttttccaaaacaaaaaaacccccccccagattcttctaatgtctcgtgggctcggagatgtgtataagagacaGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1217:32655:8718/57‑1 (MQ=60)

                     GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2262:27847:26300/151‑1 (MQ=60)

                     GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2217:51690:7837/151‑1 (MQ=60)

                     GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2262:24288:11328/151‑1 (MQ=60)

                     GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2262:24279:11344/151‑1 (MQ=60)

                     GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1265:39459:19799/151‑1 (MQ=60)

                     GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1266:22402:2105/151‑1 (MQ=60)

                     GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2260:21052:3002/151‑1 (MQ=60)

                     GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2112:36464:15347/151‑1 (MQ=60)

                     GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1118:12418:29519/151‑1 (MQ=60)

                     GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2256:11484:27293/151‑1 (MQ=60)

                     GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1129:51339:22473/151‑1 (MQ=60)

                     GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               >  LH00941:114:22FVMWLT1:2:2238:41391:8542/1‑151 (MQ=60)

                     GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGGACGCCATGGAGCACAAGCTGATCTGAGTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1265:39468:19783/151‑1 (MQ=60)

                     GGCACGCCTGATGCAGAAGTTGAAAGNGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2267:43555:16580/151‑1 (MQ=60)

                     GGCACGCCTGATGCAGAAGTGGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2166:10587:20840/151‑1 (MQ=60)

                        ACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGC                                                                                                                            <  LH00941:114:22FVMWLT1:2:2164:38368:17045/151‑1 (MQ=60)

                         CGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCAAGAACAGGACTGGCCTGCT                                                                                                                           >  LH00941:114:22FVMWLT1:2:1254:20350:21448/1‑151 (MQ=60)

                          GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:2259:10892:13970/151‑1 (MQ=60)

                          GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:1228:26193:26188/151‑1 (MQ=60)

                          GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:2156:5854:11136/151‑1 (MQ=60)

                          GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:2225:24639:14723/151‑1 (MQ=60)

                          GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:1218:41567:5227/151‑1 (MQ=60)

                          GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGCGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:2227:2137:13314/151‑1 (MQ=60)

                             TGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATC                                                                                                                       <  LH00941:114:22FVMWLT1:2:1206:5188:19879/151‑1 (MQ=60)

                             TGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATC                                                                                                                       <  LH00941:114:22FVMWLT1:2:2236:35844:23370/151‑1 (MQ=60)

                             TGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATC                                                                                                                       <  LH00941:114:22FVMWLT1:2:2263:26183:28670/151‑1 (MQ=60)

                             TGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATC                                                                                                                       <  LH00941:114:22FVMWLT1:2:2124:43610:2681/151‑1 (MQ=60)

                               ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                                     <  LH00941:114:22FVMWLT1:2:1145:48492:1849/151‑1 (MQ=60)

                               ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                                     <  LH00941:114:22FVMWLT1:2:2149:36270:26284/151‑1 (MQ=60)

                               ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                                     <  LH00941:114:22FVMWLT1:2:2149:36279:26300/151‑1 (MQ=60)

                               ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                                     <  LH00941:114:22FVMWLT1:2:2153:15265:20456/151‑1 (MQ=60)

                               ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                                     <  LH00941:114:22FVMWLT1:2:2157:34790:24459/151‑1 (MQ=60)

                               ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                                     <  LH00941:114:22FVMWLT1:2:2157:34809:24459/151‑1 (MQ=60)

                               ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                                     <  LH00941:114:22FVMWLT1:2:1145:48482:1833/151‑1 (MQ=60)

                               ATGCAGAAGGTGAAAGTGAACTCGCTGGCGGGGCTGGTGAGGTACGCCATGGAGCACAAGCGGATCTGAGTGCGACATACGGGTGCCCGAAACTGGCCGAAGCCGATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                                     <  LH00941:114:22FVMWLT1:2:2221:35040:23674/151‑1 (MQ=60)

                                  CAGAAGTTGAAAGTGAACTCGCTGGCGGGGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:1129:4051:1192/151‑1 (MQ=60)

                                  CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:1110:2997:22938/151‑1 (MQ=60)

                                  CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:1202:11900:7421/151‑1 (MQ=60)

                                  CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:2148:12861:23818/151‑1 (MQ=60)

                                     AAGTTGAAAGTGACCTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACAGACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1101:30482:12321/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGTTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGACCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2236:2146:15732/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1239:1804:2105/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2267:25832:3402/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1251:36510:12609/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1223:11151:28062/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2233:35484:25916/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1211:25518:10095/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2230:34439:13986/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1210:27746:1400/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1269:51829:16693/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2223:8683:2970/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1204:12723:1993/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1147:45441:15940/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1203:29918:28734/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2256:16319:7902/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2148:2322:24907/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2112:3589:19223/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2265:7582:4139/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1202:24852:26556/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1107:21968:6893/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2127:2812:7309/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2135:17789:20087/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1101:30492:12337/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2149:13721:18390/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATGGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2259:22827:7517/151‑1 (MQ=60)

                                     AAGTTGAAAGTGAACTCGCGGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1109:8729:5996/151‑1 (MQ=60)

                                       GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                                             <  LH00941:114:22FVMWLT1:2:1158:33792:6460/151‑1 (MQ=60)

                                       GTTGAAAGTGAACCCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                                             <  LH00941:114:22FVMWLT1:2:1158:33811:6460/151‑1 (MQ=60)

                                         TGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAG                                                                                                           <  LH00941:114:22FVMWLT1:2:2140:19379:6348/151‑1 (MQ=60)

                                         TGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAG                                                                                                           <  LH00941:114:22FVMWLT1:2:2140:21163:7902/151‑1 (MQ=60)

                                               TGAACTCGCTGTAGGAGCGGGTGAAGGACGCCATGGAGCACAAGCGGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGTGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCTGCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2142:4994:20920/151‑1 (MQ=60)

                                               TGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCC                                                                                                     <  LH00941:114:22FVMWLT1:2:2142:4985:20904/151‑1 (MQ=60)

                                                         GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                                           <  LH00941:114:22FVMWLT1:2:2108:8193:23418/151‑1 (MQ=60)

                                                           CGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCA                                                                                         <  LH00941:114:22FVMWLT1:2:2153:38063:19367/151‑1 (MQ=60)

                                                               GCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCC                                                                                     <  LH00941:114:22FVMWLT1:2:1242:48954:14211/151‑1 (MQ=60)

                                                                CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                                    <  LH00941:114:22FVMWLT1:2:2229:12908:27293/151‑1 (MQ=60)

                                                                CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                                    <  LH00941:114:22FVMWLT1:2:1170:11022:15508/151‑1 (MQ=60)

                                                                CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                                    <  LH00941:114:22FVMWLT1:2:1104:17678:24187/151‑1 (MQ=60)

                                                                CTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                                    <  LH00941:114:22FVMWLT1:2:1170:11031:15524/151‑1 (MQ=60)

                                                                        GTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGAACaggacttgtctccttatctctcttggtgagccggccccctctctggtacgccccccccccc                                                                            >  LH00941:114:22FVMWLT1:2:2166:32997:1881/1‑90 (MQ=60)

                                                                                                                                                  GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGGGATCCCTACAACAAATCTTCC  >  LH00941:114:22FVMWLT1:2:1203:17317:20007/1‑151 (MQ=60)

                                                                                                                                                  GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  LH00941:114:22FVMWLT1:2:2139:9986:28190/1‑151 (MQ=60)

                                                                                                                                                  GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  LH00941:114:22FVMWLT1:2:1238:22374:16629/1‑151 (MQ=60)

CAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  AE015451_phaM1/499057‑499353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`