Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A2 F11 I4 R1	74	758.2	33768536	99.3%	33532156	136.1

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	AE015451_phaM1	499,203	A→G	intergenic (+47/‑249)	uhpA CDS (DNA‑binding response regulator UhpA) → / → PP_0411 CDS (Polyamine ABC transporter_ ATP‑binding protein)	–/–

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	AE015451_phaM1	499,203	0	A	G	99.5%	766.1 / NA	195	intergenic (+47/‑249)	uhpA CDS (DNA‑binding response regulator UhpA)/PP_0411 CDS (Polyamine ABC transporter_ ATP‑binding protein)	–/–
Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (44/150); minor base T (0/1); total (44/151)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 195 total aligned reads displayed.

ACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGG  >  AE015451_phaM1/499070‑499330

  ccACAAGGCACGCCTGATGCAGAAGTTGAAGTTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                             <  2:10613821/137‑1 (MQ=255)

  ccACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCAt                                                                                                                             <  2:13952740/137‑1 (MQ=255)

    acaAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGc                                                                                                                           <  2:1252095/137‑1 (MQ=255)

       aGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCcg                                                                                                                        >  1:3647050/1‑137 (MQ=255)

        ggtaCGCCTGATGTAGAAGTTGAAGGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGTGTGCCCGAAACCTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  2:15986368/134‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  2:9673108/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  1:768889/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  1:8699987/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  1:6739785/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  1:974933/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  1:5174889/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  2:1131375/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  1:422389/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  2:1172851/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  2:12167984/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  2:772029/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  1:15760753/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  2:2129124/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  1:12552788/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  2:2989030/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  2:7336942/137‑1 (MQ=255)

        ggCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       <  2:3012252/137‑1 (MQ=255)

         gCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGACcgcg                                                                                                                      >  2:10155572/1‑137 (MQ=255)

             gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                 <  2:15431314/137‑1 (MQ=255)

             gCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑g                                                                                                                 <  1:3032399/137‑1 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGTCATGCCCGCGAAC‑ggg                                                                                                               >  2:16761180/1‑137 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCGGAC‑ggg                                                                                                               >  2:6991551/1‑137 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCcgc                                                                                                                       >  1:14927997/1‑130 (MQ=255)

               cTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCACGCCCGCGAAC‑agg                                                                                                               >  2:12783560/1‑137 (MQ=255)

                tGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggc                                                                                                              <  2:12915760/137‑1 (MQ=255)

                  aTGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcct                                                                                                            <  1:5495914/137‑1 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGGCCACAAAA‑Gggcctgga                                                                                                         >  2:9800498/1‑136 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCTCCGAC‑Aggactggc                                                                                                         >  2:5303854/1‑137 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCAAAC‑Gggactggc                                                                                                         >  1:2630215/1‑137 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGAGGCCCCCAAAC‑Aggccctga                                                                                                         >  2:11602761/1‑133 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                         <  1:16543233/137‑1 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                         <  1:3902759/137‑1 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                         <  1:8869281/137‑1 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                         <  1:8388139/137‑1 (MQ=255)

                     cAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggc                                                                                                         <  1:8388084/137‑1 (MQ=255)

                       gAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑Gggcctggcct                                                                                                       <  1:15419402/137‑1 (MQ=255)

                        aatttGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGACATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  1:10013963/134‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACACGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCTAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  2:11089190/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  1:1920210/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  2:5277358/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  2:4871325/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  2:6912846/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  1:11206221/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  1:16854726/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  2:3896944/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  1:5677083/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  1:6097114/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  1:11113273/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  1:10725135/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  2:3801859/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  1:12950367/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  2:8514688/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCTGGCAGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  1:1958037/137‑1 (MQ=255)

                        aaGTTGAAAGTGAACTCGCGGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGAGCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGgcctggcctg                                                                                                      <  2:3554637/137‑1 (MQ=255)

                          gTTGAAGGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAACATATGTGGGAGCGGGGATGGCCACGAAA‑Ggggccggctcagt                                                                                                    >  2:14825017/1‑132 (MQ=255)

                          gTTGAAAGTGAACTCGCTTGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCctgct                                                                                                    <  2:16756760/137‑1 (MQ=255)

                          gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGTAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCctgct                                                                                                    <  2:4533139/137‑1 (MQ=255)

                          gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATTTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCcggct                                                                                                    <  2:14190838/137‑1 (MQ=255)

                          gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCctgct                                                                                                    <  2:12450281/137‑1 (MQ=255)

                          gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCctgct                                                                                                    <  1:5058303/137‑1 (MQ=255)

                          gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCctgct                                                                                                    <  1:13612589/137‑1 (MQ=255)

                          gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCctgct                                                                                                    <  1:8521537/137‑1 (MQ=255)

                          gTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCACGCCCGCGGCC‑GGGACTGGCcttct                                                                                                    >  2:2663994/1‑137 (MQ=255)

                            tGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATACCCGCGAAA‑GGGACTGGCatgctag                                                                                                  >  2:11231504/1‑135 (MQ=255)

                             gAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGAt                                                                                                 <  1:6867178/137‑1 (MQ=255)

                              aaaGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTTGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGAtc                                                                                                <  2:3986395/137‑1 (MQ=255)

                                  tGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTa                                                                                            <  2:4955580/137‑1 (MQ=255)

                                  tGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTa                                                                                            <  1:7318283/137‑1 (MQ=255)

                                        cGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTg                                                                                      <  1:1382256/137‑1 (MQ=255)

                                            ggCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTGAGcc                                                                                  <  1:8126481/137‑1 (MQ=255)

                                            ggCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTGAGcc                                                                                  <  2:16228982/137‑1 (MQ=255)

                                              cGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTGAGCCgg                                                                                <  2:16573990/137‑1 (MQ=255)

                                                  gCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctc                                                                            <  1:6999445/137‑1 (MQ=255)

                                                  gCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctc                                                                            <  1:12137171/137‑1 (MQ=255)

                                                  gCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctc                                                                            <  1:6583159/137‑1 (MQ=255)

                                                   cTGTTGAAGTACGCCATGGAGCACAAGCTGATCGGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctct                                                                           <  1:9980427/137‑1 (MQ=255)

                                                   cTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCctct                                                                           <  2:5462153/137‑1 (MQ=255)

                                                      gTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCCCGAAC‑GGGTACTGACTGCTGATCTCTCTTGCTTAGCCGGCctccttt                                                                        >  1:10124030/1‑133 (MQ=255)

                                                        gAAGGATGCAATGGAGCACAAGCTGATCTGATGGAGACATATGGGTGCCCGAAACTTACCGAAGCCGATTTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCGCTAGGGCTGAGCCGGCCTTTTtgcg                                                                      <  2:2167217/137‑1 (MQ=255)

                                                              cGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATg                                                                <  2:16663696/137‑1 (MQ=255)

                                                               tccATGGAGCACAAGTTGATCTGATTGCGACATACGGGTGCCCGAAATTTGCCGAAGCCTATGTGGGAGTGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGc                                                               <  2:11934784/136‑1 (MQ=255)

                                                                   tGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCACGAAC‑GGGTCTGGTCTTTTGATTTCCCTGGTTTAGCCGGTCCCTTCGCAGGCATGTCCGc                                                           >  2:13154472/1‑137 (MQ=255)

                                                                   tGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCACGTCCGCGAAC‑AGGACTGGCCCGCTTAGCTCCCTTGTCTAGCCGGCCCCTTCTCGGGCATGccctc                                                           >  2:9065363/1‑135 (MQ=255)

                                                                   tGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCGCGAAC‑AGGGCTGGTCTGCTGGTCTCTGTGGCTGAGTCGGCCCCTTCGTGGGCACGcccca                                                           >  2:12637077/1‑135 (MQ=255)

                                                                           aaGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCGCGAAC‑AGGCCTGGGCTGCTGATTTCTCTGGGTGAGTCGGCCTCTTCTCGGGAATGCCCACTcccccca                                                   >  2:8095088/1‑133 (MQ=255)

                                                                             gCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCACGAAC‑AGGACCTGCCCGTCGATCTCTATTGGTTGGACCGTCTCCTCGCAGGCATGCcccccccccaaagg                                                 >  2:14232911/1‑131 (MQ=255)

                                                                                gatctgatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGACATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTc                                              >  1:1156585/1‑137 (MQ=255)

                                                                                     gatTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCACGCCCACGAACAGGACCTGG‑CTGCCGGTCTCCACTGCTGAGACGGACTCCTCGCGGGCACGTCCTCTCCCCCAAATTTCTTGGt                                         >  2:15909411/1‑137 (MQ=255)

                                                                                               tACGGTTGCCCGAAATTTGCCGAAGCTTGTGGGGGGGTGGGTGTGCTGGGGAAG‑GGGCTTGTCTTGTGGATTTTTATGGCTGATCTGTCCTCTTCGCGGCCGTTCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCaa                               <  2:2880864/137‑1 (MQ=255)

                                                                                               gaCGGGTGCCAAAACCTTGCAGAAGCCGAGGGGGGGGCGGGCATGCCCGAGAAT‑GGGCTTGGCCTGCTGATCTCTATGGCTGAGCCGTCCCCTCCGCGGGCATCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCaa                               <  2:6321576/136‑1 (MQ=255)

                                                                                               gaCGGGTACCCGAAACTTGCCGAAGCTGGGGGGGGAGAGGGCATACCAGAGAAC‑GGGCCGGGCTTGTGGATCTCGATGGCGGAGCCGACCTCTTCGCGGGCATACCCGCTCCCACAGGTTTCGTGGTTGGCTCGCaa                               <  2:13500154/136‑1 (MQ=255)

                                                                                                              ctttctgAAGCCGCTGTGGAAGCGGGCGTGCCCGAGAAT‑GGGCCTGTCTTGCTAATCTCTGTGGTGGAGCCGGCCTCTTCGTGGACATCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATccc                <  2:15033139/131‑1 (MQ=255)

                                                                                                                            ttggggAGTGGGCATACCCGCGAAT‑GGTCTTGGCCTGTTGATTGCTAGGGAGGAGCAGGCTTCTTCGGGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAgg  <  2:5973001/134‑1 (MQ=255)

                                                                                                                            tgtgGGGGTGGGCGTTCCCGAAAAG‑GGGCTTGGCTGGCTGCTTTTTGTGGTTGAGCCGTCCTCTTAGAGGTCGGCCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAgg  <  2:5605865/137‑1 (MQ=255)

                                                                                                                            tgtgGGAGCGGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGAGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAgg  <  2:12281745/137‑1 (MQ=255)

ACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAAC‑GGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGG  >  AE015451_phaM1/499070‑499330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

BRESEQ :: bam2aln output

CGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  AE015451_phaM1/499053‑499353

CGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCG                                                                                                                                                        <  LH00941:114:22FVMWLT1:2:2114:28097:12161/151‑1 (MQ=60)

CGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCG                                                                                                                                                        <  LH00941:114:22FVMWLT1:2:2114:28106:12177/151‑1 (MQ=60)

    CAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCA                                                                                                                                                    <  LH00941:114:22FVMWLT1:2:1160:17123:4299/151‑1 (MQ=60)

        CCCATCAGCACCCACAGGGCACGCCTGATGCAGAAGGTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1102:27903:3498/151‑1 (MQ=60)

        ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:2254:16744:4347/151‑1 (MQ=60)

        ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1254:39737:22970/151‑1 (MQ=60)

        ACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1140:24455:16004/151‑1 (MQ=60)

        ACCACCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTGGCCGAAGCCTATGTGGGAGCGGGCATGCC                                                                                                                                                <  LH00941:114:22FVMWLT1:2:1102:27894:3482/151‑1 (MQ=60)

         CCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCC                                                                                                                                               <  LH00941:114:22FVMWLT1:2:2202:19971:10223/151‑1 (MQ=60)

         CCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCC                                                                                                                                               <  LH00941:114:22FVMWLT1:2:2202:19952:10223/151‑1 (MQ=60)

          CATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCG                                                                                                                                              >  LH00941:114:22FVMWLT1:2:1161:20276:26092/1‑151 (MQ=60)

             CAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtc                                                                                                                                           <  LH00941:114:22FVMWLT1:2:1216:11733:12321/151‑3 (MQ=60)

             CAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtc                                                                                                                                           <  LH00941:114:22FVMWLT1:2:1130:4227:24171/151‑3 (MQ=60)

               GCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtccc                                                                                                                                         <  LH00941:114:22FVMWLT1:2:2215:26479:12625/151‑5 (MQ=60)

               GCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtccc                                                                                                                                         <  LH00941:114:22FVMWLT1:2:1201:20664:20424/151‑5 (MQ=60)

                  CCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGG                                                                                                                                      >  LH00941:114:22FVMWLT1:2:2238:11022:27197/1‑151 (MQ=60)

                  CCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGACCGCGAAAGGG                                                                                                                                      >  LH00941:114:22FVMWLT1:2:1266:39949:23722/1‑151 (MQ=60)

                   CCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCtcccacat                                                                                                                                     <  LH00941:114:22FVMWLT1:2:2108:42658:10768/151‑9 (MQ=60)

                        AGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGCAAACGGGACTGGC                                                                                                                                >  LH00941:114:22FVMWLT1:2:1144:43656:26076/1‑151 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2169:22624:24587/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1243:1684:13650/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1243:27173:3994/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1223:15311:21368/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1217:29752:8910/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2129:38017:14771/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1217:29733:8910/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2116:39237:3434/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1217:29761:8926/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1167:23299:27934/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2266:30954:7629/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1117:34190:10223/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2252:19129:17701/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1113:44220:25996/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1145:6529:12305/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1113:44202:25996/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:1108:8091:3386/151‑1 (MQ=60)

                         GGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGAGCCTGGCC                                                                                                                               <  LH00941:114:22FVMWLT1:2:2105:26794:17173/151‑1 (MQ=60)

                              GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:1151:31962:17701/151‑1 (MQ=60)

                              GCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:1133:34310:16132/151‑1 (MQ=60)

                              GCCGGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTG                                                                                                                          <  LH00941:114:22FVMWLT1:2:2103:22051:1592/151‑1 (MQ=60)

                                  GATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCT                                                                                                                      <  LH00941:114:22FVMWLT1:2:2244:16874:28622/151‑1 (MQ=60)

                                   ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                                     <  LH00941:114:22FVMWLT1:2:1222:25851:16981/151‑1 (MQ=60)

                                   ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                                     <  LH00941:114:22FVMWLT1:2:2140:38424:1897/151‑1 (MQ=60)

                                   ATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTC                                                                                                                     <  LH00941:114:22FVMWLT1:2:2252:46458:6076/151‑1 (MQ=60)

                                      CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:1270:47299:10864/151‑1 (MQ=60)

                                      CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:1270:47299:10832/151‑1 (MQ=60)

                                      CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:1270:47290:10848/151‑1 (MQ=60)

                                      CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:2108:44803:11985/151‑1 (MQ=60)

                                      CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:2265:13777:13330/151‑1 (MQ=60)

                                      CAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTAT                                                                                                                  <  LH00941:114:22FVMWLT1:2:1166:43684:4475/151‑1 (MQ=60)

                                        GAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGG                                                                                                                <  LH00941:114:22FVMWLT1:2:1137:19619:14259/151‑1 (MQ=60)

                                        GAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGG                                                                                                                <  LH00941:114:22FVMWLT1:2:2246:42695:28926/151‑1 (MQ=60)

                                         AATTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1260:15995:26364/151‑1 (MQ=60)

                                         AATTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGACATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2127:22827:15748/151‑1 (MQ=60)

                                         AAGTTGAAAGTGGACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2145:8119:27133/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2139:29789:13458/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2145:8128:27117/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2147:35530:14114/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2205:11872:22201/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2206:28458:12401/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2215:20470:11248/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:2270:35465:15796/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1225:22014:17029/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1115:36427:3594/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1133:29151:2745/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1225:22005:17045/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1232:2479:15251/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1246:33700:22089/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1248:44045:11600/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGGACGCCATGGAGCACAAGCTGATCTGATTGCGACAGACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1233:50304:8414/151‑1 (MQ=60)

                                         AAGTTGAAAGTGAACTCGCTGGCAGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGC                                                                                                               <  LH00941:114:22FVMWLT1:2:1133:48048:20712/151‑1 (MQ=60)

                                          AGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGAGAACGGGCCTGGgccgatggtctctccggat                                                                                                              >  LH00941:114:22FVMWLT1:2:2163:33293:25259/1‑132 (MQ=60)

                                           GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                                             <  LH00941:114:22FVMWLT1:2:2217:43897:8846/151‑1 (MQ=60)

                                           GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                                             <  LH00941:114:22FVMWLT1:2:2102:38128:17525/151‑1 (MQ=60)

                                           GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                                             <  LH00941:114:22FVMWLT1:2:1130:43351:1689/151‑1 (MQ=60)

                                           GTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTG                                                                                                             <  LH00941:114:22FVMWLT1:2:1215:27163:10672/151‑1 (MQ=60)

                                              GAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGC                                                                                                          <  LH00941:114:22FVMWLT1:2:1245:10781:6893/151‑1 (MQ=60)

                                                   TGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1252:21441:19911/151‑1 (MQ=60)

                                                   TGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCC                                                                                                     <  LH00941:114:22FVMWLT1:2:1211:13804:22185/151‑1 (MQ=60)

                                                         CGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCCCGAACGGGtactgactgctgatctctcttgcttagccggcctccttt                                                                                               >  LH00941:114:22FVMWLT1:2:2129:15478:11312/1‑112 (MQ=60)

                                                         CGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCG                                                                                               <  LH00941:114:22FVMWLT1:2:1124:25582:3642/151‑1 (MQ=60)

                                                             GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                                           <  LH00941:114:22FVMWLT1:2:1241:49148:6572/151‑1 (MQ=60)

                                                             GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                                           <  LH00941:114:22FVMWLT1:2:1266:11900:1721/151‑1 (MQ=60)

                                                             GGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGG                                                                                           <  LH00941:114:22FVMWLT1:2:1155:25693:24811/151‑1 (MQ=60)

                                                               CGGAGTTGGTGAAGTAAGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCTGAGACTTGCCGAGGCCTAGGTGGGAGCGGGCATGCCCACGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCA                                                                                         <  LH00941:114:22FVMWLT1:2:2149:3589:2089/151‑1 (MQ=60)

                                                                   GCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCC                                                                                     <  LH00941:114:22FVMWLT1:2:2162:25573:28798/151‑1 (MQ=60)

                                                                   GCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCC                                                                                     <  LH00941:114:22FVMWLT1:2:1240:48834:15828/151‑1 (MQ=60)

                                                                   GCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCC                                                                                     <  LH00941:114:22FVMWLT1:2:1247:28088:12369/151‑1 (MQ=60)

                                                                    CTGTTGAAGTACGCCATGGAGCACAAGCTGATCGGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCC                                                                                    <  LH00941:114:22FVMWLT1:2:2126:39598:28334/151‑1 (MQ=60)

                                                                      GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCCCAAACGGGACTGGCCctcttctccctcctgctgaatcctcctctttcccggtaagccccc                                                                                  >  LH00941:114:22FVMWLT1:2:2233:10855:29022/1‑106 (MQ=60)

                                                                      GGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCcagaaccaggccggccctatgacccctgcagtctagacagccccccccccagtactcccca                                                                                  >  LH00941:114:22FVMWLT1:2:2233:10837:29022/1‑90 (MQ=60)

                                                                                GCCCTGGAGCACAAGCTGATCTGATTGCGACACACGAGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGCTGCCCGCGAACtgatcttgccctactatctatcctgcttcttatgtctctcctctggtcctgccgctaccccagat                                                                        >  LH00941:114:22FVMWLT1:2:2251:3367:27069/1‑86 (MQ=60)

                                                                                   ATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGACATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTC                                                                     >  LH00941:114:22FVMWLT1:2:1120:29650:10880/1‑151 (MQ=60)

                                                                                                             ACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCGAGAAAGGGCCTGGtctgtttgtcttcctggctgagtaggtatcttctctgggccgtccgttcccacaaggttcgtgggtgggtcgtaaaggaacttccc                                           >  LH00941:114:22FVMWLT1:2:2261:37527:14243/1‑65 (MQ=60)

                                                                                                             ACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGGATGCCCACGAACaggccctgcctccttctctctctggcctcgccagccccttctcgggtcttccccccccccccaatttttttttttgttttcccagggaattccc                                           >  LH00941:114:22FVMWLT1:2:1269:6908:6428/1‑57 (MQ=60)

                                                                                                             ACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGAATGCCCCCGAACCGGCCTcgccttcttacctctccttcttagcccgcctccttcctggtcctccccccccccccaaattcttttgtgggcctcccaagcacccccc                                           >  LH00941:114:22FVMWLT1:2:2130:45404:19431/1‑63 (MQ=58)

                                                                                                                  CGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGaccggcccgtttgtctctcttgctgagcccgccccccctcttgcctgcccgctccccccaacttctttttttgttctccagagacttccccccccc                                      >  LH00941:114:22FVMWLT1:2:1270:26895:27149/1‑55 (MQ=60)

                                                                                                                       GCCCGAAACTTGCCGAAGCCTATGTGGGAGCGGGCATGCCCGCGAACGGGCCTGGCCctgtctcttatacacatctccgagcccacgagacagatagtagcatctgggggggggggttttttttttagggggggggggggggggggggggg                                 >  LH00941:114:22FVMWLT1:2:1141:44636:15956/1‑57 (MQ=60)

                                                                                                                                                      GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  LH00941:114:22FVMWLT1:2:2222:27089:3626/1‑151 (MQ=60)

                                                                                                                                                      GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  LH00941:114:22FVMWLT1:2:2218:32415:8654/1‑151 (MQ=60)

                                                                                                                                                      GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAAGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  LH00941:114:22FVMWLT1:2:2105:8461:29519/1‑151 (MQ=60)

                                                                                                                                                      GGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAAGTTTCGTGGTTGGCTCGCAAGGGCATTCAAATCCCGCCATTCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  LH00941:114:22FVMWLT1:2:2256:51025:27533/1‑151 (MQ=60)

CGAGCAAGACCATCAGCACCCACAAGGCACGCCTGATGCAGAAGTTGAAAGTGAACTCGCTGGCGGAGCTGGTGAAGTACGCCATGGAGCACAAGCTGATCTGATTGCGACATACGGGTGCCCGAAACTTGCCGAAGCCTATGTGGGAGCAGGCATGCCCGCGAACGGGCCTGGCCTGCTGATCTCTATGGCTGAGCCGGCCTCTTCGCGGGCATGCCCGCTCCCACAGGTTTCGTGGTTGGCTCGCAAGGGCATTCCAATCCCGCCATCCTTGTAGGGCGATCCCTACAACAAATCTTCC  >  AE015451_phaM1/499053‑499353

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 0 ≤` `ATCG/ATCG` `< 9 ≤` `ATCG/ATCG` `< 24 ≤` `ATCG/ATCG` `< 40 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`